A method of laser Raman spectrometry was developed for the detection of DNA bases.The Raman spectra of adenine(A),guanine(G),cytosine(C) and thymine(T) were collected with laser Raman spectrometer in this work.The characteristic peaks in the spectra for each DNA base were analyzed,which were used to determine the components of DNA base mixtures.Results demonstrated that the characteristic peaks mainly ranged from 175 cm-1 to 1 800 cm-1.The peaks of A and G were higher than those of C and T when the integration time and laser power were certain.The strongest Raman signals for A,G,C,and T are at 723,648,791 and 1 368 cm-1,respectively.Each DNA base could be determined by using the characteristic peaks from the mixed DNA bases within 2 min.