An ultra-performance liquid chromatography-Q exactive orbitrap high resolution mass spectrometric（UPLC Orbitrap HRMS） method was utilized for the analysis of ginsenosides compositions in different processd Panax quinquefoliums L.roots.The electrospray ionization（ESI） ion source and orbitrap mass analyzer were equipped，and negative ion mode was used for data collection.On the basis of accurate mass to charge in MS，the accurate molecular weights of ginsenosides were acquired.Based on the second stage of tandem MS data，the fragmentations of ginsenosides were obtained.The characteristic MS fragmentation pathways of different types of ginsenosides were summarized.According to these fragmentation rules，55 ginsenosides in Panax quinquefoliums L.roots were identified，among which protopanaxdiol（PPD） type ginsenosides were 28，protopanaxtriol（PPT） type ginsenosides were 17，oleanane（OLE） type ginsenosides and ocotillol（OCO） type ginsenosides were both 5.The ginsenosides compositions of PPD，PPT，OCO and OLE types in different processd Panax quinquefoliums L.roots were compared.The results obtained provided the information for influences of different processing on ginsenosides composition of Panax quinquefoliums L.roots.The research also provided a reference for the exploitation and utilization of Panax quinquefoliums L.roots，and a clarification for its bio-effectiveness material basis.