Determination of Aflatoxins in Edible Oil by High Performance Liquid Chromatography with Stirring Bar Sorptive Extraction Based on Dopamine-Graphene Oxide Complex
Determination of Aflatoxins in Edible Oil by High Performance Liquid Chromatography with Stirring Bar Sorptive Extraction Based on Dopamine-Graphene Oxide Complex[J]. 2018,37(8):913-918.
Determination of Aflatoxins in Edible Oil by High Performance Liquid Chromatography with Stirring Bar Sorptive Extraction Based on Dopamine-Graphene Oxide Complex[J]. 2018,37(8):913-918.DOI:
A method of stirring bar sorptive extraction(SBSE) based on mussel-bioinspired chemistry coating and high performance liquid chromatography with fluorescence detector(HPLC/FLD) was developed for the simultaneous determination of aflatoxin B1,B2,G1 and G2(AFB1,AFB2,AFG1 and AFG2) in edible oil.The sample was extracted by SBSE with dopamine-graphene oxide complex as solid phase extraction material prepared by mussel-bioinspired chemistry.The target compounds were separated on a Kromasil C18 column using methanol-acetonitrile-water aqueous solution(3∶3∶5 by volume,adjust pH 35 with 10%phosphoric acid) as mobile phases,and detected with a fluorescence detector.The calibration curves for AFB1,AFB2,AFG1 and AFG2 were linear in the concentration range of 0200-100 μg/L,with their correlation coefficients(r2) not less than 0998 9.The spkied recoveries for 4 aflatoxins(AFs) in edible oil samples ranged from 815% to 969%,with intra day relative standard deviations(RSDs) of 17%-34% and inter day RSDs of 19%-35%.The limits of detection(LODs) were in the range of 0025-0050 μg/L.The proposed method is efficient,sensitive and reliable,and is suitable for the determination of AFB1,AFB2,AFG1 and AFG2 in edible oil.
关键词
贻贝仿生化学多巴胺-氧化石墨烯复合物搅拌棒吸附萃取高效液相色谱/荧光检测器食用油黄曲霉毒素
Keywords
mussel-bioinspired chemistrydopamine-graphene oxide complexstirring bar sorptive extraction(SBSE)high performance liquid chromatography with fluorescence detector(HPLC/FLD)edible oilaflatoxin(AF)