The porous material O pinacolyl methylphosphonic acid imprinted polymer microspheres were synthesized through designing pre assembly system,based on the interaction between the degradation product of Soman(O pinacolyl methylphosphonic acid) molecules with methylacrylic acid and acrylamide functional monomer molecules discussed by techniques such as 31P ｛1H｝NMR spectroscopy and 1H NMR spectroscopy.Scatchard analysis showed that the maximum apparent binding amount Qmax was 661 μg/g（AM as monomer）,and a straight line was presented in the diagram of a low adsorption range(Q＜500 μg/g).In static adsorption experiment,the polymer was observed to be regularly selective to homologous five alkyl phosphonates monoalkyl esters,and the identification factors α to other compounds with different structures were decreased obviously.A solid phase extraction column using this synthesized polymer microsheres was used in the separation of trace O pinacolyl methylphosphonic acid containing a large number of interferents with a remarkable effect.Results showed that the polymer synthesized provided an effective means for analysis and identification of trace degradation products of related chemical warfare agents in the complex samples.