A method for the determination of chloramphenicol in cosmetics was developed using direct real time analysis-high resolution mass spectrometry.The sample was ultrasonically extracted with ethanol,then diluted 1∶1 by volume with water,and finally injected with a 12 Dip-it Samplers.The instrument parameter were optimized to obtain the sensitive and accurate determination for chloramphenicol.At a sample introduction rate of 0.3 mm/s,high intensity of [M-H]-ions for chloramphenicol were observed in the negative ion and Full MS-PRM mode by utilization of high purity helium gas at 500 ℃.The calibration curve for chloramphenicol was linear in the range of 0-100 mg/L with a correlation coefficient of 0.998 4.The limit of quantitation(LOQ) of the method was 3.0 mg/kg.Recoveries for chloramphenicol at three spiked levels of 3.0,6.0,30 mg/kg were in the range of 70.2%-119%,with relative standard deviations(RSD) of 8.4%-17%.With its rapid analysis,simple pretreatment steps and low matrix interference,the method was suitable for the rapid determination of chloramphenicol in cosmetics.
关键词
实时直接分析高分辨质谱化妆品氯霉素快速检测
Keywords
direct analysis in real timehigh resolution mass spectrometrycosmeticschloramphenicolrapid determination