A microarray protein chip method was established for the simultaneous detection of clenbuterol and ractopamine residues in food.Results showed that the linear ranges for clenbuterol and ractopamine were 0.07-1.2 ng/g and 0.05-0.8 ng/g,respectively.The recoveries for the two substances in pork and pig liver were in the range of 74%-132%,with relative standard deviations all less than 10%.The established method was compared with HPLC-MS,and the results obtained were identical.This method was simple,rapid and high throughput,and could be used in the detection of clenbuterol and ractopamine residues in actual samples.
关键词
蛋白芯片克伦特罗莱克多巴胺人工抗原
Keywords
protein chipclenbuterolractopamineartificial antigen