A disposable paper-based plate was developed for the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer(MALDI-TOF MS) as the existing stainless steel plate or disposable plastic plate requires a complicated cleaning process,which is easy to generate residues causing cross-contamination.Besides,it is not environmentally-friendly when these plates are recycled.Being low-cost and easy to handle after employment,the plate makes no pollution to the environment.The paper-based plate was tested in four aspects,i.e.resolution,sensitivity,reproducibility and microorganism identification.Results showed that the paper-based plate is basically consistent with the steel plate in terms of resolution and sensitivity,and it has a potential in the clinical identification of microorganism with good reproducibility.