With the continued development of green chemistry,how to apply and reflect the characteristics of green chemistry in analysis process,avoiding the secondary pollution of the analysis process and the possible harm to the analysts have received an extensive attention.Therefore,the development and use of solvents with green chemistry characteristics is one of the main work for analysts.Deep eutectic solvents(DESs) are another type of solvents with similar physical properties to ionic liquids(ILs).Compared with ILs,DESs have showed some advantages such as environmental friendliness,non-combustibility,biodegradablity,low cost and simple preparation,etc.DESs have been used as green solvents in the extraction and separation of target compounds in different samples.The preparation,properties and classification of DESs are summarized this review,and their applications in extraction and separation are also presented.