A novel method was established for the rapid detection of ephedrine in urine by portable high resolution ion mobility spectrometry with poly vinylidene fluoride microporous membrane-polydopamine-graphene oxide solid phase extraction.The new type of solid phase extraction membrane could realize a 5 min of rapid adsorption,a 10 min of elution and a 30 ms of injection detection for ephedrine by optimizing preparation process,adsorption and elution process.The results showed that good linearity was found from 0124 μg/mL to 6129 μg/mL for ephedrine with a detection limit of 960 ng/mL,and the recoveries ranged from 910% to 113%.The method could be applied in the rapid detection of ephedrine in urine,and provide an effective means for on line detection and on site monitoring of driving under the inference of drugs.