An analytical method was developed for the determination of ethoxyquin in apple,pear,grape and orange samples by high performance liquid chromatography combined with fluorescence detection.Samples were pretreated by mixing vitamin C,alkalescence adjustment,n hexane extraction and acetonitrile redissolution.Ethoxyquin residue in samples were detected by high performance liquid chromatography with fluorescence detector,and quantified by external standard method.There were good linear relationships for ethoxyquin in the concentration range of 2-100 μg/L,with their correlation coefficient(r) of 0.999 99.The limits of detection and quantitation were 0.01 mg/kg and 0.02 mg/kg,respectively.Average recoveries at four spiked levels of 0.02,0.04,0.2 and 1 mg/kg ranged from 80.4% to 108%,with relative standard deviations(RSD,n=6) of 1.4%-8.1%.With simplicity,rapidness,good repeatability and stability,this method is suitable for the determination of ethoxyquin in fruits.