A surface enhanced Raman spectroscopy（SERS）with in situ synthesized silver colloid substrate was developed for the rapid detection of amiloride hydrochloride in tablets.The silver colloid substrate for surface enhanced Raman scattering was prepared using heating paster（warm baby）as heating source,and the SERS performance was evaluated with rhodamine 6G（R6G）as a SERS probe.Results indicated that the synthesis method for silver colloid was simple and rapid,and the substrate could be fabricated within 15 min and keep for 4 h with satisfactory stability and good SERS enhancement effect.It was applied in the SERS detection of amiloride hydrochloride in tablets.There was a linear relationship between SERS intensity and amiloride hydrochloride in the concentration range of 0.8-8 mg/L,with a curve equation of y=1 395ρ-76.40（r2 =0.999 2）.The limit of detection was 0.2 mg/L,and the spiked recoveries from tablet samples were in the range of 104%-106% with relative standard deviations of 0.35%-3.5%.With the advantages of simpleness,rapidness and good stability,the developed method provides a new appraoch for the identification and rapid detection of amiloride hydrochloride in tablets.