Preparation of Ti4+ Immobilized Silica Nanocomposites and Their  Application in Separation and Enrichment of Phosphoproteins

谢青; 胡巧云; 郑琼; 林子俺

doi:doi：10.3969/j.issn.1004-4957.2019.10.011

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Preparation of Ti4+ Immobilized Silica Nanocomposites and Their Application in Separation and Enrichment of Phosphoproteins

- Preparation of Ti4+ Immobilized Silica Nanocomposites and Their Application in Separation and Enrichment of Phosphoproteins
- Vol. 38, Issue 10, Pages: 1228-1233(2019)
- 作者机构：
  
  1. 福建生物工程职业技术学院药学系
  2. 福州大学化学学院食品安全与生物分析教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：doi：10.3969/j.issn.1004-4957.2019.10.011
  CLC：
- Published：2019
- 稿件说明：
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谢青, 胡巧云, 郑琼, 林子俺. Preparation of Ti4+ Immobilized Silica Nanocomposites and Their Application in Separation and Enrichment of Phosphoproteins[J]. 2019, 38(10): 1228-1233.
DOI：

谢青, 胡巧云, 郑琼, 林子俺. Preparation of Ti4+ Immobilized Silica Nanocomposites and Their Application in Separation and Enrichment of Phosphoproteins[J]. 2019, 38(10): 1228-1233. DOI： doi：10.3969/j.issn.1004-4957.2019.10.011.

摘要

采用St-ber法，以四乙氧基硅烷(TEOS)和γ-巯丙基三甲氧基硅烷(γ-MPTS)为前驱体合成了硅球纳米粒子，并结合“巯-烯”点击反应和“一锅法”，制备得到固载Ti4+的纳米复合硅球。通过红外光谱、透射电镜等方法对材料进行表征，利用蛋白吸附实验以及凝胶电泳等方法探究了该亲和材料对磷酸化蛋白的分离富集效果。结果表明，该纳米复合材料分散性好，粒径均一，对磷酸化蛋白(α-酪蛋白)的最大吸附容量(12.27 μmol/g)远大于非磷酸化蛋白(辣根过氧化物酶，1.12 μmol/g)，且能从牛奶实际样品中分离富集磷酸化蛋白。

Abstract

The Ti4+ immobilized silica nanocomposites(SiO2-Ti4+) were fabricated by synthesizing silica nanoparticles with tetraethoxysilane(TEOS) and γ-mercaptopropyl trimethoxysilane(γ-MPTS) as precursors

followed by “thiol-ene” click reaction and “one pot” process.The morphology and structure properties of the resultant SiO2-Ti4+ were characterized by transmission electronic microscopy(TEM)

Fourier transform infrared spectroscopy(FT-IR) and X ray photoelectron spectroscopy(XPS).The adsorption behavior of the SiO2-Ti4+ towards phosphoproteins was investigated.Under the optimized conditions

the SiO2-Ti4+ showed a rather higher adsorption capacity towards phosphoproteins(α-casein，12.27 μmol/g) than non phosphoproteins(horseradishperoxidase，1.12 μmol/g).In addition

the practicality of the SiO2-Ti4+ was further demonstrated by their selective enrichment of phosphoproteins from milk samples.

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