Simultaneous Determination of Three Kinds of Cyanobacteria in Water by Ultrahigh Performance Liquid Chromatography－ Tandem Mass Spectrometry

LI Zhi-xuan; ZHAN She-xia; LI Zhi-tu; ZHANG Chang-li; ZHAO Qian-ning

doi:10.19969/j.fxcsxb.23030102

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Simultaneous Determination of Three Kinds of Cyanobacteria in Water by Ultrahigh Performance Liquid Chromatography－ Tandem Mass Spectrometry

Experimental Techniques and Methods | 更新时间：2023-07-12

- Simultaneous Determination of Three Kinds of Cyanobacteria in Water by Ultrahigh Performance Liquid Chromatography－ Tandem Mass Spectrometry
- Journal of Instrumental Analysis Vol. 42, Issue 7, Pages: 888-892(2023)
- 作者机构：
  
  澳门自来水股份有限公司，澳门 999078
- 作者简介：
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- DOI：10.19969/j.fxcsxb.23030102
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黎志轩,湛社霞,李志土等.超高效液相色谱－串联质谱法同时检测水中的3种蓝藻毒素[J].分析测试学报,2023,42(07):888-892.

LI Zhi-xuan,ZHAN She-xia,LI Zhi-tu,et al.Simultaneous Determination of Three Kinds of Cyanobacteria in Water by Ultrahigh Performance Liquid Chromatography－ Tandem Mass Spectrometry[J].Journal of Instrumental Analysis,2023,42(07):888-892.
黎志轩,湛社霞,李志土等.超高效液相色谱－串联质谱法同时检测水中的3种蓝藻毒素[J].分析测试学报,2023,42(07):888-892. DOI： 10.19969/j.fxcsxb.23030102.

LI Zhi-xuan,ZHAN She-xia,LI Zhi-tu,et al.Simultaneous Determination of Three Kinds of Cyanobacteria in Water by Ultrahigh Performance Liquid Chromatography－ Tandem Mass Spectrometry[J].Journal of Instrumental Analysis,2023,42(07):888-892. DOI： 10.19969/j.fxcsxb.23030102.

摘要

建立了超高效液相色谱－串联质谱测定水中石房蛤毒素（STX）、拟柱胞藻毒素（CYN）、鱼腥藻毒素（AnTX）3种蓝藻毒素的分析方法。通过考察色谱柱、流动相和梯度洗脱程序对分离的影响，获得了最佳的色谱、质谱条件。结果表明：采用HSS T3柱，以甲酸水溶液（0.1%）和乙腈为流动相梯度洗脱，3种目标物可获得有效分离。STX、CYN、AnTX的方法检出限（LOD）为0.018、0.009、0.013 μg/L，相关系数（,r,）均大于0.999，方法在0.50 μg/L加标水平的回收率为102% ~ 104%，相对标准偏差为1.7% ~ 5.2%。该方法灵敏、准确、简便、快速，适用于水体中3种低浓度蓝藻毒素的同时检测。

Abstract

An ultrahigh performance liquid chromatography－tandem mass spectrometry（UPLC－MS/MS） was established for the determination of three cyanotoxins，i.e. saxitoxin（STX），cylindrospermopsin（CYN） and anatoxins（AnTX） in water．The optimal chromatographic and mass spectrometric conditions were obtained through investigating the influence of chromatographic column，mobile phase and gradient elution program on the separation．The results showed that the three target analytes were effectively separated by gradient elution procedure with formic acid water（0.1%） and acetonitrile as the mobile phases．The limits of detection（LODs） of the method for STX，CYN，AnTX were 0.018，0.009 and 0.013 μg/L，while the correlation coefficients（,r,） were all higher than 0.999．The recoveries at a spiked level of 0.50 μg/L ranged from 102% to 104%，with the relative standard deviations of 1.7%－5.2%．This method is sensitive，accurate，simple and rapid，and it is suitable for the simultaneous detection of three cyanotoxins with low concentration in water.

关键词

超高效液相色谱－串联质谱（UPLC－MS/MS）鱼腥藻毒素石房蛤毒素拟柱胞藻毒素水

Keywords

ultrahigh performance liquid chromatography－tandem mass spectrometry（UPLC－MS/MS）anatoxinsaxitoxincylindrospermopsinwater

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