Simultaneous Determination of 5 Saponins in Chonglou Xiaoliu Formula by Ultra Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detection

XU Ai-li; CAO Ying-nan; ZHANG Su-zhong; QIU Jin-yan; XIE Can-hui; BI Xiao-li; XIAO Guan-lin

doi:10.19969/j.fxcsxb.22101102

您当前的位置：

首页 >

文章列表页 >

Simultaneous Determination of 5 Saponins in Chonglou Xiaoliu Formula by Ultra Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detection

Experimental Techniques and Methods | 更新时间：2023-02-28

- Simultaneous Determination of 5 Saponins in Chonglou Xiaoliu Formula by Ultra Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detection
- Journal of Instrumental Analysis Vol. 42, Issue 2, Pages: 221-226(2023)
- 作者机构：
  
  1.广东省中医药工程技术研究院，广东广州 510095
  2.广州新华学院药学院，广东广州 510520
  3.广东省中医药研究开发重点实验室，广东广州 510095
  4.广州中医药大学第五临床医学院，广东广州 510405
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.22101102
  CLC：
扫描看全文
胥爱丽,曹颖男,张素中等.超高效液相色谱－蒸发光散射检测法同时测定重楼消瘤方中5种皂苷类成分含量[J].分析测试学报,2023,42(02):221-226.

XU Ai-li,CAO Ying-nan,ZHANG Su-zhong,et al.Simultaneous Determination of 5 Saponins in Chonglou Xiaoliu Formula by Ultra Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detection[J].Journal of Instrumental Analysis,2023,42(02):221-226.
胥爱丽,曹颖男,张素中等.超高效液相色谱－蒸发光散射检测法同时测定重楼消瘤方中5种皂苷类成分含量[J].分析测试学报,2023,42(02):221-226. DOI： 10.19969/j.fxcsxb.22101102.

XU Ai-li,CAO Ying-nan,ZHANG Su-zhong,et al.Simultaneous Determination of 5 Saponins in Chonglou Xiaoliu Formula by Ultra Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detection[J].Journal of Instrumental Analysis,2023,42(02):221-226. DOI： 10.19969/j.fxcsxb.22101102.

摘要

建立了同时测定抗肿瘤中药复方——重楼消瘤方中重楼皂苷Ⅰ、重楼皂苷Ⅱ、重楼皂苷Ⅶ、柴胡皂苷a、柴胡皂苷d 5种皂苷类成分含量的超高效液相色谱－蒸发光散射检测（UPLC－ELSD）法。复方制剂经甲醇提取，采用Waters Cortecs UPLC C,18,（150 mm × 2.1 mm，1.6 μm）色谱柱分离，乙腈－水为流动相进行梯度洗脱，蒸发光散射检测器进行检测，以外标法定量。结果显示，5种皂苷类成分的分离度良好，分别在7.117 4 ~ 177.94 μg/mL、9.407 2 ~ 235.18 μg/mL、8.872 6 ~ 221.82 μg/mL、7.486 2 ~ 187.15 μg/mL、9.761 3 ~ 244.03 μg/mL范围内线性关系良好，相关系数（,r,）为0.999 2 ~ 0.999 8，检出限为0.7 ~ 1.0 μg/mL，定量下限为1.8 ~ 2.4 μg/mL；平均回收率分别为93.9%、103%、102%、104%、105%，相对标准偏差（RSD）分别为3.8%、4.0%、4.2%、3.6%、3.2%。该方法简便、快速、准确，可同时对重楼消瘤方中的5个皂苷类成分进行分析，能有效地消除杂质干扰，为重楼消瘤方的质量评价提供实验依据。

Abstract

An ultra performance liquid chromatography（UPLC） coupled with evaporative light scattering detection（ELSD） was established for the simultaneous determination of 5 saponins，i.e．polyphyllin I，polyphyllin Ⅱ，polyphyllin Ⅶ，saikosaponin a and saikosaponin d in Chonglou Xiaoliu Formula. The samples were extracted with methanol， then separated on a Waters Cortecs UPLC C,18,（150 mm × 2.1 mm，1.6 μm） column with acetonitrile－water as the mobile phase by gradient elution，finally determined by ELSD and quantified by external standard calibration method．The results showed that there were good linear relationships for 5 saponins in the ranges of 7.117 4－177.94 μg/mL，9.407 2－235.18 μg/mL，8.872 6－221.82 μg/mL，7.486 2－187.15 μg/mL and 9.761 3－244.03 μg/mL，respectively，with their correlation coefficients（,r,） of 0.999 2－0.999 8．The limits of detection and the limits of quantitation were in the ranges of 0.7－1.0 μg/mL and 1.8－2.4 μg/mL，respectively．The average recoveries were 93.9%，103%，102%，104% and 105%，with their relative standard deviations（RSDs） of 3.8%，4.0%，4.2%，3.6% and 3.2%，respectively．With the advantages of simplicity，rapidness and accuracy，the method could be applied in simultaneous analysis of 5 saponins in Chonglou Xiaoliu Formula and effective elimination of the impurity interference．And it also provides a reference for the quality evaluation on Chonglou Xiaoliu Formula．

关键词

重楼消瘤方皂苷类成分超高效液相色谱蒸发光散射

Keywords

Chonglou Xiaoliu Formulasaponinsultra performance liquid chromatographyevaporative light scattering detection

references

Su M，Gong X J，Zhou X．China J. Chin. Mater. Med. （苏敏，龚小见，周欣．中国中药杂志），2019，44（4）：675－684.

Wang C H，Jiang M M，Wang G C，Zhang X Q，Ye W C．Int. J. Pharm. Res. （王春华，姜苗苗，王国才，张晓琦，叶文才．国际药学研究杂志），2013，40（6）：678－687.

Chinese Pharmacopoeia Commission. Pharmacopoeia of the People’s Republic of China. Beijing：China Medical Science and Technology Press（中国药典委员会．中华人民共和国药典．北京：中国医药科技出版社），2020.

Wang G X，Han J，Zhao L W，Jiang D X，Liu Y T，Liu X L．Phytomedicine，2010，17（14）：1102－1105.

Nie Q，Tan J，Chen Q，Kuang G．Anti－Tumor Pharm. （聂芩，谭君，陈乾，况刚．肿瘤药学），2022，12（3）：337－343.

Li Y F，Liu Y S，Li Y S．Northwest Pharm. J. （李艳凤，刘雅舒，李艳生．西北药学杂志），2022，37（5）：186－192.

Xiao L X，Zhou H N，Long B，Jiao Z Y．Biomed. Transform. （肖丽霞，周辉年，龙勃，焦作义．生物医学转化），2021，2（4）：60－66.

Cheng H X，Duan Q Z．Shanxi J. Tradit. Chin. Med. （程海霞，段清珍．山西中医），2021，37（2）：57－60.

Huo Q Q，Sun L L，Qin L N，Wang W T．Guide of China Med. （霍秦秦，孙立丽，秦丽娜，王婉婷．中国医药指南），2020，18（9）：205－206.

Zhao F Y，Tao A E，Guan X，Qian J F，Xia C L．Chin. Tradit. Herb. Drugs（赵飞亚，陶爱恩，管鑫，钱金栿，夏从龙．中草药），2020，51（18）：4763－4770.

Zhang T，Liu H，Liu X T，Xu D R，Chen X Q，Wang Q．J. Pharm. Biomed. Anal.，2010，51（1）：114-124.

Guo Y G，Bai Y B，Li H Q，Hu G Y，Li X．Cent. South Pharm. （郭宇鸽，柏玉冰，李洪权，胡冠羽，黎旭．中南药学），2022，20（8）：1911－1915.

Ma S F，Sun J W，Gao X J，Bai M X．J. Shenyang Pharm. Univ. （马守芳，孙江伟，高晓洁，白明学．沈阳药科大学学报），2021，38（8）：821－826.

Jia R，Huang X Z，Jia G Q，Shi Y Q，Cui Z Y，Guo Z B．J. Instrum. Anal. （贾茹，黄学者，贾光群，石玉秋，崔宗岩，郭泽镔．分析测试学报），2022，41（6）：851－857.

Fan Q Y，Bai Z F，Wang X M，Wang W H．J. Instrum. Anal. （樊轻亚，白泽方，汪学猛，王万好．分析测试学报），2022，41（8）：1235－1241.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Determination of Sialic Acid in Fish by Ultra High Performance Liquid Chromatography with DMBA Derivatization

Determination of 12 Saccharides in Honey by Ultra High Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detection

Determination of Haloacetic Acids in Drinking Water Using Ultra Performance Liquid Chromatography Coupled with Solid phase Extraction

Simultaneous Screening and Quantitation of 31 Carbamate Pesticide Residues in Feed by Ultra Performance Liquid Chromatography-Orbitrap Mass Spectrometry

Related Author

No data

Related Institution

Marine Fisheries Research Institute of Zhejiang

Key Lab of Sustainable Utilization of Technology Research for Fishery Resource of Zhejiang Province

Marine Biomedical Products Engineering Technology Research Center of Zhejiang Province

College of Food Science，Fujian Agriculture and Forestry University

Technology Center of Qinhuangdao Customs

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰