Determination of 21 Anesthetics in Skin Care Products by QuEChERS/High Performance Liquid Chromatography－Tandem Mass Spectrometry

YANG Jian-ying; ZHU Hong; XU Ning; CHEN Xiao-hong; CHEN Qing; XIAO Heng

doi:10.19969/j.fxcsxb.22092203

您当前的位置：

首页 >

文章列表页 >

Determination of 21 Anesthetics in Skin Care Products by QuEChERS/High Performance Liquid Chromatography－Tandem Mass Spectrometry

Scientific Papers | 更新时间：2023-02-28

- Determination of 21 Anesthetics in Skin Care Products by QuEChERS/High Performance Liquid Chromatography－Tandem Mass Spectrometry
- Journal of Instrumental Analysis Vol. 42, Issue 2, Pages: 189-196(2023)
- 作者机构：
  
  广东省汕头市质量计量监督检测所，广东汕头 515041
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.22092203
  CLC：
扫描看全文
杨建英,朱虹,徐宁等.QuEChERS/高效液相色谱－串联质谱法测定护肤品中的21种麻醉剂[J].分析测试学报,2023,42(02):189-196.

YANG Jian-ying,ZHU Hong,XU Ning,et al.Determination of 21 Anesthetics in Skin Care Products by QuEChERS/High Performance Liquid Chromatography－Tandem Mass Spectrometry[J].Journal of Instrumental Analysis,2023,42(02):189-196.
杨建英,朱虹,徐宁等.QuEChERS/高效液相色谱－串联质谱法测定护肤品中的21种麻醉剂[J].分析测试学报,2023,42(02):189-196. DOI： 10.19969/j.fxcsxb.22092203.

YANG Jian-ying,ZHU Hong,XU Ning,et al.Determination of 21 Anesthetics in Skin Care Products by QuEChERS/High Performance Liquid Chromatography－Tandem Mass Spectrometry[J].Journal of Instrumental Analysis,2023,42(02):189-196. DOI： 10.19969/j.fxcsxb.22092203.

摘要

建立了QuEChERS/高效液相色谱－串联质谱（HPLC－MS/MS）同时快速测定护肤品中21种麻醉剂的分析方法。样品用水分散后加入含1.0%（体积分数）甲酸的乙腈溶液超声提取，经无水硫酸镁、十八烷基键合硅胶（C,18,）和N-丙基乙二胺（PSA）净化，采用具有反相（RP）、弱阳离子交换（WCX）和HILIC多种保留机制的Acclaim Mixed－Mode WCX－1色谱柱（150 mm × 2.1 mm，3 μm）分离，以20 mmol·L,-1,甲酸铵溶液（用甲酸调至pH 4.0）和乙腈（含0.035%甲酸）为流动相。在电喷雾正离子模式下，多反应监测（MRM）模式测定，基质匹配标准溶液外标法定量。21种麻醉剂在0.5 ~ 100 ng·mL,-1,质量浓度范围内线性关系良好，相关系数（,r,）均大于0.99，检出限和定量下限分别为0.1 ~ 3 μg·kg,-1,和0.3 ~ 10 μg·kg,-1,。在膏霜、乳液和凝胶3种基质中，3个不同加标水平下的平均回收率为84.3% ~ 109%，相对标准偏差为0.70% ~ 7.1%（,n ,= 6）。该方法操作简便，适用于护肤品中21种麻醉剂的日常检测。

Abstract

A QuEChERS/high performance liquid chromatography－tandem mass spectrometry（HPLC－MS/MS） was established for the rapid and simultaneous determination of 21 anesthetics in skin care products．The samples were dispersed with water，then extracted with acetonitrile solution containing 1.0%（by volume） formic acid by ultrasound extraction，and purified with anhydrous magnesium sulfate，octadecyl silane（C,18,） and primary secondary amine（PSA），and finally seperated on an Acclaim Mixed－Mode WCX－1 column（150 mm × 2.1 mm，3 μm） which could offer multiple retention mechanisms including reversed-phase（RP），weak cation-exchange（WCX） and HILIC．The mobile phases were 20 mmol·L,-1, ammonium formate（adjusting to pH 4.0） and acetonitrile（cantaining 0.035% formic acid）．The samples were detected in electrospray positive ion mode under multiple reaction monitoring（MRM） mode，and quantified by matrix-matched standard solutions and external standard method．There were good linear relationships for 21 anesthetics in the concentration range of 0.5－100 ng·mL,-1,，with their correlation coefficients（,r,） larger than 0.99．The detection limits and quantitation limits were 0.1－3 μg·kg,-1, and 0.3－10 μg·kg,-1,，respectively．The average recoveries at three spiked levels for the 21 analytes in three matrices ranged from 84.3% to 109%，with relative standard deviations of 0.70%－7.1%（,n ,= 6）．The method is simple to operate，and it is suitable for the daily detection of 21 anesthetics in skin care products.

关键词

QuEChERS高效液相色谱－串联质谱法（HPLC－MS/MS）麻醉剂护肤品

Keywords

QuEChERShigh performance liquid chromatography－tandem mass spectrometry（HPLC－MS/MS）anestheticskin care product

references

Gitman M，Barrington M J．Reg. Anesth. Pain Med.，2018，43（2）：124－130.

China Food and Drug Administration. Safety and Technical Standards for Cosmetics（国家食品药品监督总局．化妆品安全技术规范），2015.

Ayandra S J，Francielly F S J，José A F d S，Daniela D，Dosil P J．Sep. Sci. Plus，2019，2（11）：422－427.

Yang H，He S N，Yang F，Zhang B B，Yu Y Y，Chen Z G．J. Instrum. Anal. （杨晖，和素娜，杨帆，张贝贝，于艳艳，陈缵光．分析测试学报），2014，33（11）：1302－1306.

Xin H P，Wang L，Zhang X H，Gao M．China Med. Herald（辛虎平，王丽，张晓虹，高明．中国医药导报），2011，8（32）：53－54，59.

Tian Y P，Gu L L．Shanghai Meas. Test. （田玉平，顾玲玲．上海计量测试），2015，42（5）：10－11.

Guo X Y，Zhao W Z，Wang F，Xia D F，Li W T，Ma Q．China Surfactant Deterg. Cosmet. （郭项雨，赵卫哲，王锋，夏德富，李文涛，马强．日用化学工业），2020，50（9）：648－652.

Li Y J，Chen J T，Hu Y X，Ma S M，Wang Y D．Eye Sci. （李裕军，陈婕婷，胡缨贤，马淑媚，王延东．眼科学报），2022，37（4）：291－297.

Lu L，Fang F，Feng Y L，Cao L．China Surfactant Deterg. Cosmet. （芦丽，方方，冯有龙，曹玲．日用化学工业），2021，51（8）：795－801.

Shi F，Shou D，Jin M C，Wang H W，Chen X G，Zhu Y．Chin. J. Chromatogr. （石芳，寿旦，金米聪，王宏伟，陈旭光，朱岩．色谱），2022，40（2）：139－147.

Huang Y T，Zhuang Y，Qiu Y Q．J. Instrum. Anal. （黄艳婷，庄玥，邱蕴绮．分析测试学报），2020，39（12）：1533－1537.

Shen X，Gu X F，Zhou Z Y．Cent. South Pharm. （沈心，顾晓风，周震宇．中南药学），2021，19（8）：1653－1656.

Lu L，Fang F，Feng Y L，Cao L．Chin. J. Pharm. Anal. （芦丽，方方，冯有龙，曹玲．药物分析杂志），2021，41（12）：2178－2190.

Lian X H，Wang C，Meng X S，Bai H，Sun X J，Xue H Y，Ma Q．Chin. J. Anal. Chem. （连显会，王春，孟宪双，白桦，孙小杰，薛宏宇，马强．分析化学），2019，47（5）：756－764.

Guo X Q，Ye Z Y，Lei C J，Liu H B，Li Y，Han X，Cai Y H．Chin. J. Anal. Lab.

郭旭茜，叶子煜，雷春娟，刘洪斌，李颖，韩雪，蔡英华．分析试验室），2021，40（8）：948－953.

Gao P，Yang X，Mo C N，Chen R M，Zeng D D，Liu H M，Hong P Z，Zhou K，Chen Y S．J. Instrum. Anal. 高平，杨曦，莫彩娜，陈日檬，曾丹丹，刘唤明，洪鹏志，周凯，陈营寿．分析测试学报），2019，38（9）：1059－1065.

Wang D Q，Bu Y Y，Li J．Pharm. Today（王鼎乾，卜媛媛，李进．今日药学），2019，29（11）：745－748.

Mazaher A，Mohammad M M，Tayyebeh M，Abbas A，Soleiman B，Mohamed A R．J. Chromatogr. B，2018，1095：177－182.

Thermo Fisher Scientific. Acclaim Mixed－Mode WCX－1 for Separating Basic Molecules. ［2022－08－29］. https：//www.thermofisher.cn/document-connect/document-connect.html？url=https：//assets.thermofisher.cn/TFS-Assets%2FCMD%2FSpecification-Sheets%2F65840-DS_Acclaim_Mixed_Mode_WCX-1_21Feb08_LPN2024.pdfhttps://www.thermofisher.cn/document-connect/document-connect.html?url=https://assets.thermofisher.cn/TFS-Assets%2FCMD%2FSpecification-Sheets%2F65840-DS_Acclaim_Mixed_Mode_WCX-1_21Feb08_LPN2024.pdf.

Thermo Fisher Scientific. Acclaim Mixed－Mode HPLC Columns. ［2022－08－29］. https：//assets.thermofisher.cn/TFS-Assets/CMD/brochures/br-22032-acclaim-mixed-mode-br22032-en.pdfhttps://assets.thermofisher.cn/TFS-Assets/CMD/brochures/br-22032-acclaim-mixed-mode-br22032-en.pdf.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Residue Determination of Niclosamide-olamine in Twenty Two Kinds of Agricultural Products by UPLC-MS/MS

Simultaneous Determination of 16 Coumarins and Vanillins and Their Derivatives in Rice by QuEChERS-Isotope Internal Standard/Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

Screening of 166 Pesticide Residues in Tea Based on Ultra High Performance Liquid Chromatography-Quadrupole/Electrostatic Field Orbitrap High Resolution Mass Spectrometry

Determination of 18 Synthetic Cannabinoids in Chocolate by QuEChERS/High Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry

Related Author

No data

Related Institution

College of Horticulture and Landscape，Tianjin Agricultural University

Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables；Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable，Ministry of Agriculture and Rural Affairs；Analysis and Test Center，Chinese Academy of Tropical Agricultural Sciences

Tea Research Institute，Chinese Academy of Agricultural Sciences

Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation；Hainan Institute for Food Control

Changsha Institute for Food and Drug Control

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰