Study on Chemical Differences of Alkaloids from Fritillariae Cirrhosae Bulbus and Their Counterfeits Based on Liquid Chromatography-Mass Spectrometry Technology
Scientific Papers|更新时间:2025-08-28
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Study on Chemical Differences of Alkaloids from Fritillariae Cirrhosae Bulbus and Their Counterfeits Based on Liquid Chromatography-Mass Spectrometry Technology
Journal of Instrumental AnalysisVol. 44, Issue 9, Pages: 1900-1911(2025)
LIU Shan-shan,NIU Chuang,YANG Hui-ying,MA Jun-jun,XIANG Shu-qin,XU Shuang-shuang,QIAN Chong,WANG Wei,ZHANG Mei.Study on Chemical Differences of Alkaloids from Fritillariae Cirrhosae Bulbus and Their Counterfeits Based on Liquid Chromatography-Mass Spectrometry Technology[J].Journal of Instrumental Analysis,2025,44(09):1900-1911.
LIU Shan-shan,NIU Chuang,YANG Hui-ying,MA Jun-jun,XIANG Shu-qin,XU Shuang-shuang,QIAN Chong,WANG Wei,ZHANG Mei.Study on Chemical Differences of Alkaloids from Fritillariae Cirrhosae Bulbus and Their Counterfeits Based on Liquid Chromatography-Mass Spectrometry Technology[J].Journal of Instrumental Analysis,2025,44(09):1900-1911. DOI: 10.12452/j.fxcsxb.25061007.
Study on Chemical Differences of Alkaloids from Fritillariae Cirrhosae Bulbus and Their Counterfeits Based on Liquid Chromatography-Mass Spectrometry Technology
The chemical differences of alkaloids from Fritillariae Cirrhosae bulbus(Songbei,Qingbei,Lubei) and their common counterfeits(
Fritillaria ussuriensis
,
Fritillaria
walujewii
,
Fritillaria palldiflora
) were studied to clarify the quantitative identification index of them. High performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) was used to determine the alkaloids of genuine and common counterfeit products. The differential compounds were analyzed by multivariate statistical methods,and identified by comparison of reference information,matching of mass spectrometry database and literature mass spectrometry information. The quantitative identification compounds were confirmed by combining their distributions in different samples. A HPLC-MS/MS method was established and verified by methodology.
Twenty different metabolites in Songbei and
Fritillaria ussuriensis
,Qingbei and
Fritillaria
walujewii
,Lubei and
Fritillaria palldiflora
were identified. Peimine and peiminine can be used as quantitative indicators to distinguish Songbei and
Fritillaria ussuriensis
,and imperialine and edpetiline can be used as quantitative indicators to distinguish Qingbei and
Fritillaria
walujewii
,Lubei and
Fritillaria palldiflora
. HPLC-MS/MS technology combined with chemometrics can effectively clarify the different chemical components of genuine and common counterfeit
Fritillariae Cirrhosae bulbus. The quantitative identification indexes were clearly defined,and it provided reference for the subsequent quantitative identification and quality control of Fritillariae Cirrhosae bulbus.
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Chinese Pharmacopoeia Commission . Pharmacopoeia of the People's Republic of China : Volume 1 (国家药典委员会 . 中华人民共和国药典 : 一部. 北京:中国医药科技出版社) , 2020 .
Su J H , Zhang C , Sun L , Ma S C , Xing Y W . J. Chin. Pharm. Sci. , 2023 , 32 ( 5 ): 406 - 416 .
Xie M D , Tian L Y , Gui X J , Shi J H , Wang J M , Yue Y S , Zhang L , Wang Y L , Yao J , Li X L , Liu R X . Chin. Tradit. Herb. Drugs (谢梦迪,田亮玉,桂新景,施钧瀚,王君明,岳佑凇,张璐,王艳丽,姚静,李学林,刘瑞新. 中草药), 2022 , 53 ( 8 ): 2490 - 2498 .
Fan L H , He L , Tan C Q , Tian Y , Zhang C L , Wu C J , Huang Y L . Chin. J. Exp. Tradit. Med. Formulae (范林宏,何林,谭超群,田瑶,张春玲,吴纯洁,黄永亮. 中国实验方剂学杂志), 2022 , 28 ( 3 ): 131 - 138 .
Zhang F L , Liu W , Mao J F , Yin Q , Lan Q K , Liu Q , Zhang Y R , Chen L F , Yang X F , Luo X M , Chen M , Guo LA , Lei S R . China J. Chin. Mater. Med. (张富丽,刘炜,毛建霏,尹全,兰青阔,刘茜,张义蓉,陈龙飞,杨晓凤,罗晓梅,陈敏,郭灵安,雷绍荣. 中国中药杂志), 2021 , 46 ( 13 ): 3337 - 3348 .
Zhou Q , Lei Q Y , Zhao J N , Fang Q M , Wang S . World Chin. Med. (周琪,雷乾娅,赵军宁,方清茂,王曙. 世界中医药), 2020 , 15 ( 2 ): 225 - 230 .
Guo F L , Xiong R , Liu X H , Liu H S , Zhang Y , Zhao T X , Wang N . Mod. Chin. Med. (郭凤柳,熊蕊,刘晓慧,刘会双,张毅,赵同欣,王娜. 中国现代中药), 2021 , 23 ( 9 ): 1590 - 1594 .
Shi Y , Liu W , Wei F , Ma S C . Chin. J. Pharm. Anal. (石岩,刘薇,魏锋,马双成. 药物分析杂志), 2024 , 44 ( 7 ): 1222 - 1232 .
Zhu H L . Identification of Adulteration in Fritillariae Cirrhosae Bulbus Based on MLPA-HRM Assay . Wuhan : Hubei University of Chinese Medicine(朱海兰. 基于MLPA-HRM法检测川贝母掺伪的研究. 武汉:湖北中医药大学) , 2021 .
Yang T C , Xie H M , Xie H G , Gong P Z , Liu Y F , Wang S . West China J. Pharm. Sci. (仰铁锤,谢慧敏,谢慧淦,龚盼竹,刘翼飞,王曙. 华西药学杂志), 2020 , 35 ( 3 ): 265 - 269 .
Yang J , Li J , Xue W N , Wang A M , He B , Wang Y L , Li Y J , Xi X L , Liu T . Chin. Tradit. Pat. Med. (杨健,李靖,薛维娜,王爱民,何彬,王永林,李勇军,席晓岚,刘亭.中成药), 2020 , 42 ( 5 ): 1262 - 1268 .
Lan Q K , Zhao X , Chen R , Liu Z H , Zhu Z , Wang C , Shen X L , Liu N , Wang Y , Chen L Z , Wang Y . Chin. J. Pharm. Anal. (兰青阔,赵新,陈锐,刘征辉,朱珠,王成,沈晓玲,刘娜,王燕,陈利智,王永. 药物分析杂志), 2019 , 39 ( 3 ): 551 - 556 .
Liu X X , Li J , Zhang Y B , Peng G F , Lan Y Y , Li Y J , Wang Y L , Yang C , Liu T . Chin. J. Pharm. Anal. (刘香香,李靖,张煜彬,彭高发,兰燕宇,李勇军,王永林,杨畅,刘亭. 药物分析杂志), 2019 , 39 ( 10 ): 1844 - 1851 .
Liu R X , Hao X J , Zhang H J , Zhang L , Gui X J , Lin Z Z , Luo C N , Tian L Y , Wang Y L , Feng W H , Yao J , Li X L . China J. Chin. Mater. Med. (刘瑞新,郝小佳,张慧杰,张璐,桂新景,林兆洲,罗崇念,田亮玉,王艳丽,冯文豪,姚静,李学林. 中国中药杂志), 2020 , 45 ( 14 ): 3441 - 3451 .
Zhou T , Fu S B , Xie H M , Xie H G , Ye L M , Wang S , Ye B G . West China J. Pharm. Sci. (周婷,付绍兵,谢慧敏,谢慧淦,叶利明,王曙,叶本贵. 华西药学杂志), 2021 , 36 ( 2 ): 193 - 197 .
Che P , Liu J S , Qi Y D , Qiang T Y , Song Y C , Wei X P , Liu H T , Zhang B G . China J. Chin. Mater. Med. (车朋,刘久石,齐耀东,强亭燕,宋奕辰,魏雪苹,刘海涛,张本刚.中国中药杂志), 2020 , 45 ( 6 ): 1393 - 1398 .
Zhang M R , Fu S B , Xie H M , Xie H G , Gong P Z , Wang S , Ye B G . West China J. Pharm. Sci. (张梦蕊,付绍兵,谢慧敏,谢慧淦,龚盼竹,王曙,叶本贵. 华西药学杂志), 2022 , 37 ( 1 ): 67 - 74 .
Zhu X M , Zhou T , Wang S , Ye B G , Singla R K , Tewari D , Atanasov A G , Wang D D , Liu S M . Separations , 2023 , 10 : 75 .
An Y L , Li J Y , Wei W L , Li Y , Zhang J Q , Yao C L , Bi Q R , Wang S , Zeng Z D , Guo D A . Phytomedicine , 2024 , 135 : 156193 .
Zhou J L , Xin G Z , Shi Z Q , Ren M T , Qi L W , Li H J , Li P . J. Chromatogr. A , 2010 , 1217 ( 45 ): 7109 - 7122 .