Screening of New Pollutants in Biological Samples：A Combined Purification Method Based on Dialysis and Gel Permeation Chromatography

LOU Xiao-han; TONG Yu-jun; CHENG Fei; YOU Jing

doi:10.12452/j.fxcsxb.24052365

您当前的位置：

首页 >

文章列表页 >

Screening of New Pollutants in Biological Samples：A Combined Purification Method Based on Dialysis and Gel Permeation Chromatography

Scientific Papers | 更新时间：2024-08-08

- Screening of New Pollutants in Biological Samples：A Combined Purification Method Based on Dialysis and Gel Permeation Chromatography
- Journal of Instrumental Analysis Vol. 43, Issue 8, Pages: 1212-1219(2024)
- 作者机构：
  
  暨南大学环境与气候学院，广东广州 511443
- 作者简介：
- 基金信息：
- DOI：10.12452/j.fxcsxb.24052365
  CLC： O657.7;X132
- Published：15 August 2024，
  
  Received：23 May 2024，
  
  Revised：28 June 2024，
- 稿件说明：
移动端阅览
娄小涵,佟宇俊,程飞,游静.生物样品中新污染物筛查：透析和凝胶渗透色谱联用净化方法[J].分析测试学报,2024,43(08):1212-1219.

LOU Xiao-han,TONG Yu-jun,CHENG Fei,YOU Jing.Screening of New Pollutants in Biological Samples：A Combined Purification Method Based on Dialysis and Gel Permeation Chromatography[J].Journal of Instrumental Analysis,2024,43(08):1212-1219.
娄小涵,佟宇俊,程飞,游静.生物样品中新污染物筛查：透析和凝胶渗透色谱联用净化方法[J].分析测试学报,2024,43(08):1212-1219. DOI： 10.12452/j.fxcsxb.24052365.

LOU Xiao-han,TONG Yu-jun,CHENG Fei,YOU Jing.Screening of New Pollutants in Biological Samples：A Combined Purification Method Based on Dialysis and Gel Permeation Chromatography[J].Journal of Instrumental Analysis,2024,43(08):1212-1219. DOI： 10.12452/j.fxcsxb.24052365.

摘要

以河蚬为代表，建立了一种能有效去除生物样品中脂肪及其它内源性干扰物，并保留性质广泛的新污染物的净化方法。选择理化性质迥异的35种污染物（log

为-1.30~8.41），优化生物样品萃取、透析及凝胶渗透色谱（GPC）分离方法，并验证了方法可行性。优化的样品前处理方法为：样品先后采用正己烷、丙酮、二氯甲烷混合溶液（体积比2∶2∶1）和乙腈加速溶剂萃取，提取液经4次透析后再用GPC净化，目标物使用气相色谱-质谱和液相色谱-串联质谱进行分析。采用香兰素-磷酸法测定每一净化步骤后样品的脂肪含量，并结合色谱-质谱全扫描分析，检验干扰物的去除效率。结果表明，净化后河蚬提取物中残余的脂肪含量小于1.06%，GPC中待测分析物的流出时间为8~20 min，平均回收率为55.1% ± 13.0%，相对标准偏差为1.4%~9.2%。净化后的河蚬生物样品中脂肪和部分内源性物质被有效去除，宽泛程的目标物的回收率均达到环境样品痕量新污染物检测的要求，所建方法可用于生物样品非目标筛查的前处理。

Abstract

In the present study，a cleanup method using a combination of dialysis and gel permeation chromatography（GPC） was developed to remove lipids as well as natural hormones from biological samples with the Asian clam（

Corbicula fluminea

） as the model organism. To effectively screen new pollutants across different categories in biological samples，the procedures of sample extraction，dialysis，and GPC separation were optimized. This method effectively removed lipids and other endogenous interferences from biological samples while retaining a broad range of new pollutants. We selected 35 contaminants with diverse physicochemical properties（log

ranging from -1.30 to 8.41） and evaluated their recoveries after each processing step and measured the lipid contents throughout the entire cleanup procedure. The optimized extraction method was to use accelerated solvent extraction（ASE） with a mixture of hexane，acetone and dichloromethane（2∶2∶1，volume ratio） and acetonitrile，sequentially. The extracts were subjected to four rounds of dialysis，and then were purified using GPC. Depending on their physicochemical properties，target analytes were analyzed by gas chromatography-mass spectrometry（GC-MS） or liquid chromatography-tandem mass spectrometry（LC-MS/MS）. Furthermore

，a vanillin-phosphoric acid method was applied to measure lipid content after each purification step. At the same time，removal efficiency of the interferences was also verified using chromatography-mass spectrometry analyses in scan mode. Results after the combined dialysis and GPC treatment showed the residual lipid content in sample extraction was less than 1.06%. The elution time for target analytes in GPC was determined to be between 8 to 20 min. Average recoveries was 55.1%±13.0%，with relative standard deviations of 1.4%–9.2%. In conclusion，the developed method effectively removed lipids and some endogenous substances from clam extracts. Meanwhile，the recoveries of all target analytes have met the requirements for trace environmental sample analysis，showing the feasibility of using this method to prepare biota samples for non-target analysis.

关键词

生物样品透析凝胶渗透色谱净化非目标分析

Keywords

biota sampledialysisgel permeation chromatographycleanupnon-target analysis

references

Liu P，Huang H M，Yu T，Chen S Y，Bi J P，Ding H，Zeng T J，Wu X W，Luo Z Q. Environ. Monit. Forewarning（刘沛，黄慧敏，余涛，陈诗雅，毕军平，丁华，曾恬静，吴先伟，罗志琴. 环境监控与预警），2022，14（5）：27-30，70.

Guo Q W，Wang Q，Zhang H D，Wang X B. Chemistry（郭庆伟，王倩，张海东，王修保. 化学通报），2024，87（1）：78-85.

Ruan T，Jiang G B. Bull. Chin. Acad. Sci.（阮挺，江桂斌. 中国科学院院刊），2020，35（1）：1328-1336.

Burton G A，Rosen G，Chadwick D B，Greenberg M S，Taulbee W K，Lotufo G R，Reible D D. Environ. Pollut.，2012，162：449-456.

Simon E，Lamoree M H，Hamers T，Weiss J M，Balaam J，de Boer J，Leonards P E. Environ. Sci. Technol.，2010，44（21）：8322-8329.

Sonnich M，Jarle K，Stepan B，Torunn E，Asbjørn S. J. Chromatogr. A，2005，1062（2）：255-268.

Simon E，Lamoree M H，Hamers T，De Boer J. TrAC-Trends Anal. Chem.，2015，67：179-191.

Wang L Q，He L M，Zeng Z L，Chen J X. J. Chin. Mass Spectrom. Soc.（王立琦，贺利民，曾振灵，陈建新. 质谱学报），2011，32（6）：321-332.

Little J L，Wempe M F，Buchanan C M. J. Chromatogr. B，2006，833（2）：219-230.

Agüera A，López S，Fernández-Alba A R，Contreras M，Crespo J，Piedra L. J. Chromatogr. A，2004，1045（1/2）：125-135.

Liu X Y，Chen X Y，Zhong D F. J. Chin. Mass Spectrom. Soc.（刘晓云，陈笑艳，钟大放. 质谱学报），2017，38（4）：388-399.

Du J，Lü B，Zhu P，Miao H，Wu Y N. Chin. J. Chromatogr.（杜娟，吕冰，朱盼，苗虹，吴永宁. 色谱），2013，31（8）：739-746.

Wong H，Giesy J，Siu W，Lam P. Arch. Environ. Contam. Toxicol.，2005，48：575-586.

Ji X X，Shi Z H，Cao Y Z，Wang N，Pang G F. J. Instrum. Anal.（纪欣欣，石志红，曹彦忠，王娜，庞国芳. 分析测试学报），2009，28（12）：1433-1439.

Manirakiza P，Covaci A，Schepens P. J. Food Comp. Anal.，2001，14（1）：93-100.

Zhou X J，Li W，Xu H，Zhao Y Q，Tian Y，Chang Y W. Mod. Instrum.（周相娟，李伟，许华，赵玉琪，田野，常宇文. 现代仪器），2009，15（1）：1-4.

Fang Z P，Yu B，Cong H L，Liu H W. J. Instrum. Anal.（房志鹏，于冰，丛海林，刘虎威. 分析测试学报），2021，40（6）：836-842.

Song X，Hang X Y，Wang Q，Wang L，Feng X Q，Mao L. J. Instrum. Anal.（宋鑫，杭学宇，王芹，王露，冯晓青，茅力. 分析测试学报），2016，35（5）：539-544.

Liu T，Lu L Z，Zhou X H，Dai Y F，Zhao Q L，Ni L B，Jiang L B，Wu Y. J. Food Saf. Qual.（刘婷，陆羚子，周小涵，戴意飞，赵巧灵，倪鲁波，蒋玲波，吴俣. 食品安全质量检测学报），2024，15（3）：199-207.

Li M，Zhang L，Yu T T，Fan Z Y. Mod. Food Technol.（李珉，张莉，余婷婷，范志勇. 现代食品科技），2018，34（9）：256-262.

Huang C，Chen D P. Chin. Med. Devi. Inf.（黄超，陈渡平. 中国医疗器械信息），2016，22（7）：62-64.

Cai X Y，Zhang L，Ren X，Zhang L. Int. J. Pharm. Res.（蔡晓瑶，张莉，任翔，张磊. 国际药学研究杂志），2016，43（4）：744-747.

Strandberg B，Bergqvist P A，Rappe C. Anal. Chem.，1998，70（3）：526-533.

Song X，Hang X Y，Mao L. Phys. Test. Chem. Anal.：Chem. Anal.（宋鑫，杭学宇，茅力.理化检验-化学分册），2016，52（2）：238-243.

Wang S M，Chen S L，Cui Y B. J. Hydrol.（王少梅，陈少莲，崔奕波. 水生生物学报），1993，17（2）：193-196.

Yu S D. Proceedings of the 2006 Annual Conference of the Chinese Society of Environmental Sciences（Volume 2）.（俞是聃. 中国环境科学学会 2006 年学术年会优秀论文集（下卷）），2006.

Zhao X X. Application of Homing Pigeons as a Biological Sampler for the Monitoring of Atmospheric POPs in Datong City. Guangzhou：Jinan University（赵晓曦. 信鸽作为生物采样器在大同市大气持久性有机污染物监测中的应用.广州：暨南大学），2019.

Anschau A，Caruso C S，Kuhn R C，Franco T T. Brazil. J. Chem. Eng.，2017，34：19-27.

Tong Y J，Zhao X X，Li H Z，Pei Y Y，Ma P，You J. Chemosphere，2022，307：135679.

Quan C X，Liu X Y，Liu Y M，Su J X. Sci. Technol. Food Ind.（全春喜，刘秀颖，刘玉梅，苏景秀. 食品工业科技），2011，32（3）：312-315.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Research on Status of Column Fillers for Gel Permeation Chromatography

Determination of Cefquinome Residues in Tissues of Livestock and Aquatic Products by High Performance Liquid Chromatography Tandem Mass Spectrometry with Gel Permeation Chromatography

Simultaneous Determination of 20 Kinds of Organophosphorus Pesticide Residues in Fruit Traditional Chinese Medicines

Determination of 167 Pesticide Residues in Animal-originated Foodstuffs by Combined Purification of Gel Permeation Chromatography and Solid-phase Extraction Coupled with Gas Chromatography-Tandem Mass Spectrometry

Related Author

刘虎威

丛海林

于冰

房志鹏

刘世娟

王海涛

徐雅芫

彭惠惠

Related Institution

青岛大学化学化工学院

材料科学与工程学院

生物医用材料与工程研究院

武昌理工学院生命科学学院

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰