Study on HPLC Fingerprint and Quantity Transfer Rule of Benchmark Sample of Qingjin Huatan Decoction

TANG Yu-chen; HU Yun-fei; SHA Xin; CHEN Wei; ZHOU Gui-fang; LI Wen-long

doi:10.12452/j.fxcsxb.24041003

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Study on HPLC Fingerprint and Quantity Transfer Rule of Benchmark Sample of Qingjin Huatan Decoction

Scientific Papers | 更新时间：2024-11-04

- Study on HPLC Fingerprint and Quantity Transfer Rule of Benchmark Sample of Qingjin Huatan Decoction
- Journal of Instrumental Analysis Vol. 43, Issue 9, Pages: 1389-1397(2024)
- 作者机构：
  
  1.天津中医药大学中药制药工程学院，天津 301617
  2.现代中医药海河实验室，天津 301617
  3.上海真仁堂药业有限公司，上海 200000
- 作者简介：
- 基金信息：
- DOI：10.12452/j.fxcsxb.24041003
  CLC： O657.63;R284.1
- Published：15 September 2024，
  
  Received：10 April 2024，
  
  Revised：03 June 2024，
- 稿件说明：
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汤瑜晨,胡云飞,沙鑫,陈伟,周桂芳,李文龙.经典名方清金化痰汤基准样品HPLC指纹图谱及量值传递研究[J].分析测试学报,2024,43(09):1389-1397.

TANG Yu-chen,HU Yun-fei,SHA Xin,CHEN Wei,ZHOU Gui-fang,LI Wen-long.Study on HPLC Fingerprint and Quantity Transfer Rule of Benchmark Sample of Qingjin Huatan Decoction[J].Journal of Instrumental Analysis,2024,43(09):1389-1397.
汤瑜晨,胡云飞,沙鑫,陈伟,周桂芳,李文龙.经典名方清金化痰汤基准样品HPLC指纹图谱及量值传递研究[J].分析测试学报,2024,43(09):1389-1397. DOI： 10.12452/j.fxcsxb.24041003.

TANG Yu-chen,HU Yun-fei,SHA Xin,CHEN Wei,ZHOU Gui-fang,LI Wen-long.Study on HPLC Fingerprint and Quantity Transfer Rule of Benchmark Sample of Qingjin Huatan Decoction[J].Journal of Instrumental Analysis,2024,43(09):1389-1397. DOI： 10.12452/j.fxcsxb.24041003.

摘要

基于建立的高效液相色谱（HPLC）指纹图谱结合多指标成分含量的测定方法，研究了清金化痰汤（QJHTD）的量值传递规律。制备15批QJHTD基准样品，建立其HPLC指纹图谱并进行相似度评价，确定共有峰；对QJHTD基准样品中4种指标性成分（栀子苷、芒果苷、黄芩苷、甘草酸）进行含量测定，分析饮片-基准样品的量值传递规律。结果表明，15批QJHTD基准样品指纹图谱的相似度均大于0.98。标定了26个共有峰，并对各共有峰进行药材归属。指标性成分栀子苷、芒果苷、黄芩苷、甘草酸的含量分别为44.60~76.82 mg

∙

-1

、98.04~145.25 mg

∙

-1

、46.82~75.64 mg

∙

-1

、81.60~126.63 mg

∙

-1

，转移率分别为32.91%~62.42%、2

1.78%~35.93%、29.98%~57.12%、27.51%~49.34%。15批QJHTD基准样品的出膏率为16.98%~25.19%，平均出膏转移率为67.19%。结合指纹图谱、多成分含量测定以及出膏率，研究经典名方QJHTD基准样品的量值传递规律，可为后续制剂质量控制提供参考。

Abstract

The HPLC fingerprint combined with a multi index component content determination method was established to study the quantity-quality tranmitting in Qingjin Huatan Decoction（QJHTD）. 15 batches of QJHTD substances benchmark samples were prepared，and HPLC fingerprint spectra of QJHTD substances benchmark samples were established，evaluated the similarity and determined common peaks. A method for determining the content of four indicator components（geniposide，mangiferin，baicalin，and glycyrrhetinic acid） in the QJHTD substances benchmark samples was established，and a study was conducted on the quantity-quality tranmitting between the crude herbs and substance benchmarks. The results showed that the similarity between the fingerprint spectra of 15 batches of QJHTD substances benchmark samples and the reference spectra was greater than 0.98. 26 common peaks were demarcated and the herbs were assigned to each common peak. Peaks of 10（geniposide），17，23，24 were exclusive to Zhizi（

Gardeniae

Fructus，GF），peaks of 11（mangiferin），12，13 were exclusive to Zhimu（

Anemarrhenae

Rhizoma，AR），peaks of 19（baicalin） was exclusive to Huangqin（

Scutellariae

Radix，SR），peaks of 25（glycyrrhetinic acid） was exclusive to Gancao（

Glycyrrhiza

Radix，GR）. In addition，peaks of 1-4，6，9 were the common peaks of SR，Jiegeng（

Platycodonis

Radix，PR），Sangbaipi（

Mori

Cortex，MC），Beimu（

Fritillariae Thunbergii

Bubus，FTB），AR，Gualouzi（

Trichosanthis

Semen，TS），Juhong（

Citri Exocarpium

Rubrum，CER），Fuling（Poria，P） and GR. Peaks of 5 was the common peaks of PR，FTB，AR，TS，CER，P and GR. Peaks of 7 was the common peaks of SR，PR，Maidong（

Ophiopogonis

Radix，OR），MC，FTB，TS，CER，P and GR. Peaks of 8 w

as the common peaks of SR，PR，OR，MC，FTB，AR，TS，CER，P and GR. Peaks of 14 and 20 were the common peaks of PR，OR，MC，FTB. Peaks of 15 was the common peaks of PR，OR，MC. Peaks of 16，21，22 were the common peaks of PR，OR，MC，FTB，TS，P and GR. Peaks of 18 was the common peaks of SR，GF，PR，OR，MC，FTB，TS，P and GR. Peaks of 26 was the common peaks of PR，MC. The index components of geniposide，mangiferin，baicalin and glycyrrhizic acid were 44.60-76.82 mg

∙

-1

，98.04-145.25 mg

∙

-1

，46.82-75.64 mg

∙

-1

and 81.60-126.63 mg

∙

-1

，respectively. Transfer rate were 32.91%-62.42%，21.78%-35.93%，29.98%-57.12% and 27.51%~49.34%. The yield of 15 batches of QJHTD samples ranged from 16.98% to 25.19%，and the average yield of transfer was 67.19%. Combined with fingerprint，multi-component contents determination and paste yield，the transfer rule of QJHTD benchmark sample was studied to provide reference for the quality control of preparation of classical prescription.

关键词

清金化痰汤出膏率指纹图谱量值传递含量测定高效液相色谱

Keywords

Qingjin Huatan Decoctionextract yieldfingerprint chromatogramquantity-quality tranmittingcontent determinationhigh performance liquid chromatography

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