Comparison of Enhancement Properties of Different Shapes of Nanosilver Sol Substrates and Its Application in Identification and Quantification of Food-borne Spores
Scientific Papers|更新时间:2024-12-13
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Comparison of Enhancement Properties of Different Shapes of Nanosilver Sol Substrates and Its Application in Identification and Quantification of Food-borne Spores
Journal of Instrumental AnalysisVol. 43, Issue 12, Pages: 1919-1926(2024)
TU Qian-cheng,TIAN Jia-qi,ZHU Yao-di,LI Miao-yun,ZHAO Li-jun,LIU Shi-jie,LIANG Dong.Comparison of Enhancement Properties of Different Shapes of Nanosilver Sol Substrates and Its Application in Identification and Quantification of Food-borne Spores[J].Journal of Instrumental Analysis,2024,43(12):1919-1926.
TU Qian-cheng,TIAN Jia-qi,ZHU Yao-di,LI Miao-yun,ZHAO Li-jun,LIU Shi-jie,LIANG Dong.Comparison of Enhancement Properties of Different Shapes of Nanosilver Sol Substrates and Its Application in Identification and Quantification of Food-borne Spores[J].Journal of Instrumental Analysis,2024,43(12):1919-1926. DOI: 10.12452/j.fxcsxb.24031302.
Comparison of Enhancement Properties of Different Shapes of Nanosilver Sol Substrates and Its Application in Identification and Quantification of Food-borne Spores
-DPA)在3种芽孢拉曼光谱中的出峰强度差异明显,是区别3种食源性芽孢的主要特征峰。基于SERS数据对上述3种芽孢的结构进行解析,并结合聚类分析法和K近邻算法实现了3种芽孢的快速识别。最后,通过构建枯草芽孢杆菌不同浓度对数与其1 537 cm
-1
处SERS峰强度之间线性关系的数字化定量方程,实现了食源性芽孢的快速定量。
Abstract
In this paper,the enhancement effects of three kinds of nano-silver sol substrates with different morphologies on
Bacillus subtilis
,
Bacillus cereus
and
Clostridium perfringens
were prepared and investigated,and the flower-shaped nano-silver (AgNF) with the best enhancement effect was selected for surface enhanced Raman scattering (SERS) analysis. The results showed that the peak intensity of calcium pyridine dicarboxylate (Ca
2+
-DPA) in Raman spectra of three kinds of spores was obviously different,which was the main characteristic peak to distinguish three kinds of food-borne spores. Based on SERS data,the structures of the above three kinds of spores were analyzed,and the rapid identification of the three kinds of spores was realized by combining cluster analysis and K nearest neighbor algorithm. Finally,by constructing the digital quantitative equation of the linear relationship between the logarithm of different concentrations of
Bacillus subtilis
and the SERS peak intensity at 1 537 cm
-1
,the rapid quantification of food-borne spores was realized.
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Related Author
LIANG Dong
LIU Shi-jie
ZHAO Li-jun
TU Qian-cheng
TIAN Jia-qi
ZHU Yao-di
LI Miao-yun
康燕
Related Institution
College of Food Science and Technology,Henan Agricltural University