Analysis of Chemical Constituents in <italic style="font-style: italic">Jixueteng</italic> Formula Extract by UPLC-Q-TOF-MS/MS

XIE Can-hui; JIA De-zheng; XU Ai-li; WU Min-shan; XIAO Guan-lin; BI Xiao-li; ZHANG Su-zhong; CAO Ying-nan

doi:10.12452/j.fxcsxb.23120738

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Analysis of Chemical Constituents in Jixueteng Formula Extract by UPLC-Q-TOF-MS/MS

Scientific Papers | 更新时间：2024-04-18

- Analysis of Chemical Constituents in Jixueteng Formula Extract by UPLC-Q-TOF-MS/MS
- Journal of Instrumental Analysis Vol. 43, Issue 4, Pages: 511-522(2024)
- 作者机构：
  
  1.广州中医药大学第五临床医学院，广东广州 510405
  2.广东省中医药工程技术研究院广东省中医药研究开发重点实验室，广东广州 510095
  3.广州新华学院　药学院，广东广州 510520
- 作者简介：
- 基金信息：
- DOI：10.12452/j.fxcsxb.23120738
  CLC： O657.63;R282.6
- Published：15 April 2024，
  
  Received：07 December 2023，
  
  Revised：31 January 2024，
- 稿件说明：
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谢灿辉,贾德政,胥爱丽,邬旻珊,肖观林,毕晓黎,张素中,曹颖男.UPLC-Q-TOF-MS/MS法分析鉴定鸡血藤中药复方提取物的化学成分[J].分析测试学报,2024,43(04):511-522.

XIE Can-hui,JIA De-zheng,XU Ai-li,WU Min-shan,XIAO Guan-lin,BI Xiao-li,ZHANG Su-zhong,CAO Ying-nan.Analysis of Chemical Constituents in Jixueteng Formula Extract by UPLC-Q-TOF-MS/MS[J].Journal of Instrumental Analysis,2024,43(04):511-522.
谢灿辉,贾德政,胥爱丽,邬旻珊,肖观林,毕晓黎,张素中,曹颖男.UPLC-Q-TOF-MS/MS法分析鉴定鸡血藤中药复方提取物的化学成分[J].分析测试学报,2024,43(04):511-522. DOI： 10.12452/j.fxcsxb.23120738.

XIE Can-hui,JIA De-zheng,XU Ai-li,WU Min-shan,XIAO Guan-lin,BI Xiao-li,ZHANG Su-zhong,CAO Ying-nan.Analysis of Chemical Constituents in Jixueteng Formula Extract by UPLC-Q-TOF-MS/MS[J].Journal of Instrumental Analysis,2024,43(04):511-522. DOI： 10.12452/j.fxcsxb.23120738.

摘要

应用超高效液相色谱-四极杆-飞行时间串联质谱（UPLC-Q-TOF-MS/MS）技术定性鉴别鸡血藤中药复方提取物的化学成分。采用Waters ACQUITY UPLC BEH C

色谱柱（100 mm×2.1 mm，1.7 μm），以0.1%甲酸水溶液-乙腈为流动相进行梯度洗脱，电喷雾离子源正、负离子模式扫描。通过质谱数据库、化合物裂解规律，并结合相关文献进行鉴别。共鉴别出82种化合物，以黄酮类、苯丙素类、萜类化合物为主，包括黄酮类22种、苯丙素类24种、萜类25种、酚酸类4种、甾体类6种、氨基酸类1种，各药材组成复方后首次发现的新化学成分有10种。UPLC-Q-TOF-MS/MS技术为鉴别鸡血藤中药复方中化学成分提供了简便、快速、准确的方法，鉴定得到的各个化学成分涵盖了组方中各味药材的主要成分，可为该复方的质量标准及药效物质研究提供实验依据和理论基础。

Abstract

The chemical components of the

Jixueteng

formula extract were qualitatively identified using ultra-high performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry（UPLC-Q-TOF-MS/MS） technology. A Waters ACQUITY UPLC BEH C

chromatographic column（100 mm×2.1 mm，1.7 μm） was employed，with a mobile phase consisting of 0.1% formic acid solution-acetonitrile using a gradient elution. The flow rate was set at 0.3 mL/min and the column temperature at 30 ℃. MS analysis was based on electrospray ion source with positive and negative ion mode scanning. The identification of compounds was conducted through the mass spectrometry database，compound fragmentation patterns，and relevant literature. In this experiment，a total of 82 compounds were identified，mainly including flavonoids，phenylpropanoids，and terpenoids. Among them，there were 22 types of flavonoids，24 types of phenylpropanoids，25 types of terpenoids，4 types of phenolic acids，6 types of steroids，and 1 type of amino acids. Additionally，10 new chemical components were discovered for the first time when the herbal ingredients were combined to form a compound. UPLC-Q-TOF-MS/MS technology provides a convenient，fast，and accurate method for identifying chemical components in the

Jixueteng

formula extract. The identified chemical constituents can cover the main active ingredients of the herbal compound，providing experimental evidence and theoretical foundation for quality standardization and pharmacological research of the compound.

关键词

UPLC-Q-TOF-MS/MS鸡血藤中药复方提取物化学成分裂解规律

Keywords

UPLC-Q-TOF-MS/MSJixueteng formula extractchemical constituentsfragmentation pattern

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Analysis of Chemical Constituents in Jixueteng Formula Extract by UPLC-Q-TOF-MS/MS

DOI：10.12452/j.fxcsxb.23120738

摘要

Abstract

关键词

Keywords

references