An QuEChERS/gas chromatography triple quadrupole mass spectrometry(GC-MS/MS) was developed for the simultaneous determination of 28 organophosphorus pesticide residues in Amomum villosum.The sample was firstly extracted with acetonitrile containing 1% acetic acid(by volume),and then purified with a mixed sorbent of 900 mg MgSO4,150 mg PSA,100 mg C18 and 25 mg GCB.After being concentrated and filtered,the extract was analyzed by GC-MS/MS in multiple reactions monitoring(MRM) mode and quantified by the matrix matched standard calibration curve method.Results showed that the calibration curves for 28 organophosphorus pesticides detected were linear in the range of 0.05-1.00 mg/L with correlation coefficients(r2) more than 0.99.The average recoveries at spiked levels of 0.2,0.4,1.0 mg/kg for all target compounds in the samples were in the range of 821%-104%,with relative standard deviations(RSD) no more than 15%.The limits of detection(LODs) ranged from 0.005 mg/kg to 0.010 mg/kg,and the limits of quantitation(LQDs) were between 0.010 mg/kg and 0.033 mg/kg.With simplicity,rapidness,good sensitivity,accuracy and precision,the method could provide a fast,efficient and reliable analytical approach for improving the quality and safety of Amomum villosum.