An ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) with Captiva EMR-Lipid pretreatment screening technique was established for the simultaneous analysis of 51 drug residues in pork,chicken and eggs.2.0 g of each sample was extracted with Mcllvaine-Na2EDTA buffer and acidified acetonitrile,then purified with Captiva EMR-Lipid,and finally detected by UPLC-MS/MS with electrospray ionization in positive ion scanning under multiple reaction monitoring mode.Some compounds were quantified by internal standard method,and the others were quantified by external standard method.Results showed that there were good linear relationships for 51 drugs in the range of 0.5-10 μg/L with correlation coefficients(r) not less than 0999 0.The limits of detection and quantitation were 2.5 μg/kg and 5.0 μg/kg,respectively.Average recoveries for all compounds in pork,chicken and egg samples at spiked levels of 5,10,20 μg/kg were between 60% and 110%,with relative standard deviations(n=5) lower than 10%.With simple operation and high accuracy,the method was suitable for screening of multiple drug residues in pork,chicken and eggs.