The blood's metabolism could be used to provide a precise evaluation on the post surgery physiological status,which poses an urgent technical demand for a point of care test for global metabolomic profiling.Therefore,the practical value of conductive polymer spray ionization mass spectrometry(CPSI-MS) in the multiplex monitoring of endogenous metabolites in a trace volume of whole blood was presented in this paper.The fabricated conductive polymer was composed of carbon nanotube(CNT) and polymethyl methacrylate(PMMA).After applying a high voltage of 4.5 kV for generating charged droplet spray,trace volume of extraction solvent(methanol-H2O,1∶1,5 μL) was enough to extract,desorb and ionize over 600 endogenous metabolites and lipids in the only 1 μL of diluted dried blood spot deposited on the tip,covering more than 20 fundamental metabolism pathways including the energy metabolism,amino acids metabolism,nucleotides metabolism,and lipids metabolism,etc.To give a proof of concept illustration,the C57BL/6 mice experiencing the hepatectomy were selected as the model objects.The individual differences in physiological recovery were precisely characterized by capturing hundreds of metabolites changes with a period of 48 h.Finally,a panel of metabolites mainly in the Krebs cycle,arginine metabolism,glycerolipids metabolism,and glycerophosphorylcholine metabolism could give a prognostic reference for the functional recovery of the mice from the hepatectomy.