A single cell inductively coupled plasma mass spectrometry(SC-ICP-MS) was adopted to determine the intrinsic metal elements such as chromium(Cr),manganese(Mn),iron(Fe),copper(Cu) and zinc(Zn) in an individual cell in this article.Meanwhile,the reaction gas flow rate and the quadrupole rejection parameter q(RPq) of the analytes in dynamic reaction cell(DRC) mode were optimized for accurate determination.Besides,the effects of sample flow rate,cell density and dwell time on the detection of SC-ICP-MS were investigated.The accuracy for the quantitative results of SC-ICP-MS was validated by using the results of ICP-MS determination after cell resuspension,cell lysis by ultrasonic probe and cell digestion with concentrated nitric acid.Experimental results showed that the accuracy of quantitative Zn and Cu in single cell determined by SC-ICP-MS could be evaluated by the results of ICP-MS determination after cell lysis by ultrasound. The determined Fe and Cr in single cells could be verified by the results of cell acid digestion ICP-MS. In the absence of cell reference materials, a multi-angle validation for the quantitative results of SC-ICP-MS is necessary. Studies have shown that SC-ICP-MS could be better used for the analysis of related elements in single cell.