A high performance capillary electrophoresis（HPCE） method was established for the separation and quatitative determination of saikosaponin a and saikosaponin d in Bupleuri Radix by using sulfobutyl ether-β-cyclodextrin（SBE-β-CD）and β-cyclodextrin（β-CD） as dual cyclodextrin（CD） system.Experimental conditions,such as the concentration of running buffer,the types and concentration of CDs,separation voltage and sample medium,were investigated.The results indicated that saikosaponin a and saikosaponin d could be baseline separated on an uncoated fused sillica capillary column（60 cm×50 μm i.d.,effective length:53 cm） using 20 mmol/L tris（hydroxymethyl）aminomethane-20 mmol/L SBE-β-CD-25 mmol/Lβ-CD（pH 9.9） as running buffer.The separation voltage was set at 15 kV and the detection wavelength was 210 nm.Samples were injected into capillary by gravity for 10 s and p-nitrobenzoic acid was used as the internal standard.Under the optimal conditions,the calibration curves of saikosaponin a and saikosaponin d were linear in the concentration ranges of 60-300 mg/L（r=0.999 7） and 20-100 mg/L （r=0.999 5）,respectively.The average recoveries of saikosaponin a and saikosaponin d were 102% and 100%,respectively,with RSD of 1.2% and 2.3%.The HPCE method was convenient,rapid and well repeatable,and was suitable for the quantitative analysis of saikosaponin a and saikosaponin d in Bupleuri Radix.