二维薄层色谱-表面增强拉曼散射联用技术用于饮料及水果中糖精钠的快速分析

康燕; 孙琳; 吴婷; 杜一平

doi:doi：10.3969/j.issn.1004-4957.2020.02.012

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二维薄层色谱-表面增强拉曼散射联用技术用于饮料及水果中糖精钠的快速分析

更新时间：2023-05-25

- 二维薄层色谱-表面增强拉曼散射联用技术用于饮料及水果中糖精钠的快速分析
- Rapid Analysis of Saccharin Sodium in Beverage and Fruit Based on Two dimensional Thin Layer Chromatography Coupled with Surface Enhanced Raman Scattering
- 分析测试学报 2020年39卷第2期页码：246-251
- 作者机构：
  
  华东理工大学化学与分子工程学院分析测试中心
- 作者简介：
- 基金信息：
  
  上海市科技创新行动计划(17142202600)
- DOI：doi：10.3969/j.issn.1004-4957.2020.02.012
  中图分类号：
- 纸质出版：2020
- 稿件说明：
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康燕, 孙琳, 吴婷, 杜一平. 二维薄层色谱-表面增强拉曼散射联用技术用于饮料及水果中糖精钠的快速分析[J]. 分析测试学报, 2020,39(2):246-251.

康燕, 孙琳, 吴婷, 杜一平. Rapid Analysis of Saccharin Sodium in Beverage and Fruit Based on Two dimensional Thin Layer Chromatography Coupled with Surface Enhanced Raman Scattering[J]. 2020, 39(2): 246-251.
康燕, 孙琳, 吴婷, 杜一平. 二维薄层色谱-表面增强拉曼散射联用技术用于饮料及水果中糖精钠的快速分析[J]. 分析测试学报, 2020,39(2):246-251. DOI： doi：10.3969/j.issn.1004-4957.2020.02.012.

康燕, 孙琳, 吴婷, 杜一平. Rapid Analysis of Saccharin Sodium in Beverage and Fruit Based on Two dimensional Thin Layer Chromatography Coupled with Surface Enhanced Raman Scattering[J]. 2020, 39(2): 246-251. DOI： doi：10.3969/j.issn.1004-4957.2020.02.012.

摘要

采用高稳定、高灵敏的纳米金/多孔二氧化硅复合材料作为SERS基底，构建了一种二维薄层色谱-表面增强拉曼散射(TLC-SERS)联用方法，该技术结合了薄层色谱(TLC)的快速分离功能和SERS定性定量分析的优点，提高了分离能力。采用对巯基苯胺作为探针分子研究了基底的均匀性和批-批重复性。Mapping扫描实验表明，24个测量点峰强度的相对标准偏差(RSD)为5.9%，说明该基底具有良好的点-点重复性。9个批次基底信号值的RSD为75%。将该基底用于糖精钠的检测，线性范围为0.1~200 mg/L

检出限为0.06 mg/L。用于实际样品测定，检测出饮料中糖精钠为141 mg/L，水果中为18 mg/L，10 min内能完成分析测试。该方法灵敏度高，特异性强，前处理方式简单，实现了饮料及水果中糖精钠的快速检测。

Abstract

A two dimensional thin layer chromatography-surface enhanced Raman scattering(TLC-SERS) method was constructed with highly stable and sensitive nanogold/porous silica composites as SERS substrate.This technique improved the separation capability by combining the rapid separation function of TLC and the qualitative and quantitative analysis function of SERS.The homogeneity and batch reproducibility of the substrate were studied using a p-aminothiophenol as probe.Mapping experiment showed that the substrate had a good point-point repeatability

with a relative standard deviation(RSD) of 5.9% for the peak intensities at 24 measuring points.Moreover

the RSD for 9 batches of substrates was 75%.The linear range of the substrate for the detection of saccharin sodium was 0.1-200 mg/L with the detection limit of 0.06 mg/L.The substrate was applied in the detection of saccharin sodium in actual samples

with the target concentrations in beverage and fruit of 141 mg/L and 18 mg/L

respectively.The whole analytical process could be completed within 10 min.With the advantages of high sensitivity

strong specificity and simple pretreatment

the method could realize the rapid determination of saccharin sodium in drinks and fruits.

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