A QuEChERS/high performance liquid chromatography-tandem mass spectrometric(HPLC-MS/MS)method was developed for the determination of 61 kinds of hormone residues in soil.Samples were extracted with acetonitrile

and then purified using QuEChERS method.Analysis on the targets was performed by electrospray ionization mass spectrometry in positive or negative mode

multiple reaction monitoring(MRM) mode was used for collection

and matrix matched standard curve method was selected for quantitation.Results showed that 61 analytes showed good linearities in their respective ranges

with correlation coefficients(r2) between 0.991 8 and 0.999 8.The limits of detection(LOD

S/N≥3) were 0.01-2.3 μg/kg while the limits of quantitation(LOQ

S/N≥10) were 0.03-7.5 μg/kg.Recoveries for the target analytes at spiked levels of 10

50 and 200 μg/kg ranged from 62.6% to 102%

with relative standard deviations(n=6) of 1.0%-11%.The method is simple

rapid

replicable and high stable

and could meet the requirements for determination of 61 hormone residues in soil.