Determination of 19 Triazole Fungicides in Chrysanthemum morifolium by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry Combined with Selective Accelerated Solvent Extraction
A selective accelerated solvent extraction method combined with ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was developed for the simultaneous determination of 19 triazole fungicides in Chrysanthemum morifolium.The sample was extracted with acetonitrile
and the mixed dispersive sorbents(PSA
GCB and C18) were dispersed in the extract to reduce the effects of interfering components during the extraction procedure.After concentration
the extract was separated on a BEH C18 column using 2 mmol/L ammonium formate containing 0.1% formic acid and methanol as mobile phases
and detected under multi-reaction monitoring mode in electrospray positive ion mode.Matrix matched method was used for quantitative analysis.There were good linear relationships for 19 triazole fungicides in the range of 1.0-100.0 μg/L with their correlation coefficients(r2) not less than 0.99.The limits of detection and quantitation were 0.6-3.0 μg/kg and 2.1-10.0 μg/kg
respectively.Under the optimal conditions
the average recoveries for those compounds at three spiked levels of 10
50 and 100 μg/kg ranged from 70.3% to 95.3%
with relative standard deviations(RSD) of 0.60%-12%.This method is simple
reproducible and sensitive
and is applicable for the simultaneous determination of triazole fungicides in Chrysanthemum morifolium samples.