超分子溶剂分散液液微萃取/超高效液相色谱-串联质谱法测定鱼血中13种硝基咪唑类药物残留

王春; 顾传坤; 马强; 王长海

doi:doi：10.3969/j.issn.1004-4957.2019.03.001

您当前的位置：

首页 >

文章列表页 >

超分子溶剂分散液液微萃取/超高效液相色谱-串联质谱法测定鱼血中13种硝基咪唑类药物残留

- 超分子溶剂分散液液微萃取/超高效液相色谱-串联质谱法测定鱼血中13种硝基咪唑类药物残留
- Determination of Thirteen Nitroimidazole Residues in Fish Blood by Supramolecular Solvent based Dispersive Liquid-Liquid Microextraction/Ultra performance Liquid Chromatography-Tandem Mass Spectrometry
- 分析测试学报 2019年38卷第3期页码：263-269
- 作者机构：
  
  1. 南京农业大学资源与环境科学学院
  2. 中国检验检疫科学研究院
- 作者简介：
- 基金信息：
  
  国家重点研发计划（2016YFF0203702）
- DOI：doi：10.3969/j.issn.1004-4957.2019.03.001
  中图分类号：
- 纸质出版：2019
- 稿件说明：
移动端阅览
王春, 顾传坤, 马强, 王长海. 超分子溶剂分散液液微萃取/超高效液相色谱-串联质谱法测定鱼血中13种硝基咪唑类药物残留[J]. 分析测试学报, 2019,38(3):263-269.

王春, 顾传坤, 马强, 王长海. Determination of Thirteen Nitroimidazole Residues in Fish Blood by Supramolecular Solvent based Dispersive Liquid-Liquid Microextraction/Ultra performance Liquid Chromatography-Tandem Mass Spectrometry[J]. 2019, 38(3): 263-269.
王春, 顾传坤, 马强, 王长海. 超分子溶剂分散液液微萃取/超高效液相色谱-串联质谱法测定鱼血中13种硝基咪唑类药物残留[J]. 分析测试学报, 2019,38(3):263-269. DOI： doi：10.3969/j.issn.1004-4957.2019.03.001.

王春, 顾传坤, 马强, 王长海. Determination of Thirteen Nitroimidazole Residues in Fish Blood by Supramolecular Solvent based Dispersive Liquid-Liquid Microextraction/Ultra performance Liquid Chromatography-Tandem Mass Spectrometry[J]. 2019, 38(3): 263-269. DOI： doi：10.3969/j.issn.1004-4957.2019.03.001.

摘要

建立了一种基于超分子溶剂分散液液微萃取技术测定鱼血中13种硝基咪唑类药物的超高效液相色谱-串联质谱方法。样品用正辛醇、四氢呋喃和水形成的超分子溶剂萃取，以乙腈-水为流动相进行梯度洗脱，采用Waters ACQUITY UPLC BEH C18（2.1 mm ×50 mm×1.7 μm）色谱柱分离。在电喷雾正离子模式下，采用多反应监测模式进行定性及定量分析。结果表明，13种硝基咪唑类药物在各自范围内线性关系良好，相关系数（r2）大于0.998，检出限为0.05~0.2 μg/L，定量下限为0.1~0.5 μg/L。在低、中、高3个加标水平下

13种硝基咪唑类药物的平均回收率为88.4%~105%，相对标准偏差（n=6）为4.3%~11%。该方法简便快速、准确可靠，可用于鱼血中硝基咪唑类药物残留的分析检测。

Abstract

A novel method was developed for the determination of thirteen nitroimidazoles in fish blood by ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS） with supramolecular solvent based dispersive liquid-liquid microextraction.The target compounds in fish blood were extracted with a supramolecular solvent consisted of octanol

tetrahydrofuran and water.The analytes were separated on a Waters ACQUITY UPLC BEH C18 column（2.1 mm×50 mm×1.7 μm） by gradient elution with acetonitrile-water as mobile phases.The qualitative and quantitative analyses were conducted by electrospray ionization mass spectrometry in positive ion mode under multiple reaction monitoring（MRM） mode.Experimental results demonstrated that good linearities for the analytes were observed in their respective concentration ranges with correlation coefficients（r2） larger than 0.998.The limits of detection（LOD） and the limits of quantitation（LOQ） were in the ranges of 0.05-0.2 μg/L and 0.1-0.5 μg/L

respectively.Recoveries for 13 nitroimidazoles at three spiked levels ranged from 88.4% to 105% with relative standard deviations（n=6） of 4.3%-11%.The method was simple

rapid

accurate and reliable

and could be used for the analysis of nitroimidazole residues in fish blood samples.

关键词

Keywords

references

浏览量

257

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

QuEChERS/超高效液相色谱-串联质谱法测定软胶囊型中成药中11种兴奋剂含量

超高效液相色谱-串联质谱法测定硅胶腕带中17种双酚类化合物

尿液中12种苯二氮卓类药物的聚二甲基硅氧烷海绵辅助分散液液微萃取/GC-MS法检测

可生物降解地膜中紫外吸收剂在土壤-玉米系统中的释放、迁移与累积特征

微塑料热裂解-气相色谱-质谱检测中的脂质干扰问题识别、验证方法与应对策略