基于超表面等离子共振生物传感器亲和力评价平台的开发

李裕鸿; 周翰霖; 刘钢; 黄丽萍

doi:null

您当前的位置：

首页 >

文章列表页 >

基于超表面等离子共振生物传感器亲和力评价平台的开发

更新时间：2025-07-02

- 基于超表面等离子共振生物传感器亲和力评价平台的开发
- Development of a meta surface plasmon resonance biosensor platform for affinity evaluation
- 分析测试学报 2025年
- 作者机构：
  
  1.华中科技大学生命科学与技术学院，湖北武汉 430074
  2.南京师范大学食品与制药工程学院，江苏南京 210000
  3.牡丹江师范学院生命科学与技术学院，黑龙江牡丹江 157011
  4.量准（武汉）生命科技有限公司，湖北武汉 430070
- 作者简介：
  
  黄丽萍，博士，副教授，研究方向：生物传感检测技术与器件的研发，E-mail: lphuang@njnu.edu.cn
- 基金信息：
  
  国家自然科学基金(32471472);广州国家实验室专项项目(GZNL2023A03006)
- DOI：
  中图分类号：
- 稿件说明：
移动端阅览
李裕鸿, 周翰霖, 刘钢, 黄丽萍. 基于超表面等离子共振生物传感器亲和力评价平台的开发[J/OL]. 分析测试学报, 2025.

LI Yuhong, ZHOU Hanlin, LIU Logan Gang, HUANG Liping. Development of a meta surface plasmon resonance biosensor platform for affinity evaluation[J/OL]. Journal of instrumental analysis, 2025.
李裕鸿, 周翰霖, 刘钢, 黄丽萍. 基于超表面等离子共振生物传感器亲和力评价平台的开发[J/OL]. 分析测试学报, 2025. DOI：

LI Yuhong, ZHOU Hanlin, LIU Logan Gang, HUANG Liping. Development of a meta surface plasmon resonance biosensor platform for affinity evaluation[J/OL]. Journal of instrumental analysis, 2025. DOI：

摘要

本文基于超表面等离子共振（Meta-surface plasmon resonance

MetaSPR）生物传感器构建了一种新的亲和力检测评价方法，利用高灵敏的MetaSPR芯片，同时构建高精度微流控流路式和高通量微孔板无管路式亲和力检测平台，弥补了现有亲和力检测方法的不足。研究中，制备了具有纳米孔阵列结构的MetaSPR芯片，并通过扫描电子显微镜和酶标仪对其物理结构与光学特性进行表征。通过采用不同折射率的甘油溶液对SPR芯片进行测试，结果显示该芯片对折射率变化具有极高的灵敏度，响应信号与折射率变化之间呈现出良好的相关关系（R

=0.995）。利用WeSPR系列检测仪，无需复杂光学元件，对多种分子对进行亲和力检测，成功获得了结合速率常数(

)、解离速率常数(

)和解离平衡常数(

)等动力学参数，且与之前报道的数据一致。结果表明，本研究构建的两种检测平台均能够高灵敏、实时、无标记地监测分子间的结合与解离过程，具有优异的准确性、稳定性与普适性，为生物分子相互作用研究提供了一种高效、灵敏、便携且低成本的技术手段，在生命科学、药物研发与筛选等领域具有广阔的应用前景。

Abstract

In this study

a new affinity detection and evaluation method is constructed based on the Meta-surface plasmon resonance (MetaSPR) biosensor

which utilizes a highly sensitive MetaSPR chip and simultaneously constructs a high-precision microfluidic flow-through and high-throughput microtiter plate pipeless affinity detection platform

which makes up for the shortcomings of the existing affinity detection methods.methods.In the study

MetaSPR chips with nanopore array structure were prepared and characterized by scanning electron microscop

e and enzyme marker for their physical structure and optical properties.The SPR chip was tested by using glycerol solutions with different refractive indexes

and the results showed that the chip was extremely sensitive to changes in refractive index

and a good correlation was presented between the response signal and the change in refractive index (R2=0.995).The kinetic parameters such as binding rate constant (

)

dissociation rate constant (

) and dissociation equilibrium constant (

) were successfully obtained using the WeSPR series of detector for affinity detection of a variety of molecular pairs without the need for complex optical components and were in agreement with the previously reported data.The results show that both assay platforms constructed in this study are capable of monitoring the intermolecular binding and dissociation processes in a highly sensitive

real-time and label-free manner

with excellent accuracy

stability and universality

providing an efficient

sensitive

portable and low-cost technological means for the study of biomolecular interactions

which is of broad application prospects in the fields of life sciences

drug discovery and screening.

关键词

Keywords

references

Oostindie S C , Lazar G A , Schuurman J , Parren P W H I . Avidity in antibody effector functions and biotherapeutic drug design [J ] . Nature Reviews Drug Discovery , 2022 , 21 ( 10 ): 715 - 735 .

Lupu L-M , Wiegand P , Holdschick D , Mihoc D , Maeser S , Rawer S , Völklein F , Malek E , Barka F , Knauer S , Uth C , Hennermann J , Kleinekofort W , Hahn A , Barka G , Przybylski M . Identification and affinity determination of protein-antibody and protein-aptamer epitopes by biosensor-mass spectrometry combination [J ] . International Journal of Molecular Sciences , 2021 , 22 ( 23 ): 12832 .

Xu S , Shen L , Zhang M , Jiang C , Zhang X , Xu Y , Liu J , Liu X . Surface-based multimodal protein–ligand binding affinity prediction [J ] . Bioinformatics , 2024 , 40 ( 7 ): btae413 .

Zhu W , Wang Y , Lv L , Wang H , Shi W , Liu Z , Zhou M , Zhu J , Lu H . Universal chimeric fcγ receptor t cells with appropriate affinity for igg1 antibody exhibit optimal antitumor efficacy [J ] . Acta Pharmaceutica Sinica B , 2023 , 13 ( 5 ): 2071 - 2085 .

Tabatabaei M S , Ahmed M . Enzyme-linked immunosorbent assay (elisa) [J ] . Methods Mol Biol , 2022 , 2508 : 115 - 134 .

Capelli D , Scognamiglio V , Montanari R . Surface plasmon resonance technology: Recent advances, applications and experimental cases [J ] . TrAC Trends in Analytical Chemistry , 2023 , 163 : 117079 .

Zhou J , Wang Y , Zhang G-J . State-of-the-art strategies of surface plasmon resonance biosensors in clinical analysis: A comprehensive review [J ] . Coordination Chemistry Reviews , 2024 , 520 : 216149 .

Yang , Y , Dai , Z , Zou , X . Y . Application of surface plasmon resonance technology in protein-protein interaction studies [J ] . Journal of Instrumental Analysis .

杨彦 , 戴宗 , 邹小勇 . 表面等离子体共振技术在蛋白质-蛋白质相互作用研究中的应用 [J ] . 分析测试学报) , 2009 , ( 11 ): 1344 - 1350 .

Ashrafi T M S , Mohanty G . Surface plasmon resonance sensors: A critical review of recent advances, market analysis, and future directions [J ] . Plasmonics , 2025 , 10.1007/s11468-024-02740-4( http://dx.doi.org/10.1007/s11468-024-02740-4(

Petryayeva E , Krull U J . Localized surface plasmon resonance: Nanostructures, bioassays and biosensing—a review [J ] . Analytica Chimica Acta , 2011 , 706 ( 1 ): 8 - 24 .

McOyi M P , Mpofu K T , Sekhwama M , Mthunzi-Kufa P . Developments in localized surface plasmon resonance [J ] . Plasmonics , 2024 , 10.1007/s11468-024-02620-x( http://dx.doi.org/10.1007/s11468-024-02620-x(

Gupta B D , Kant R . [invited] recent advances in surface plasmon resonance based fiber optic chemical and biosensors utilizing bulk and nanostructures [J ] . Optics & Laser Technology , 2018 , 101 : 144 - 161 .

Fan H , Li R , Chen Y , Da Q , Xiong C , Zhang Y , Qin Z , Liu G L , Huang L . Sample-to-answer point-of-care testing platform for quantitative detection of small molecules in blood using a smartphone-and microfluidic-based nanoplasmonic biosensor [J ] . Chemical Engineering Journal , 2024 , 501 : 157495 .

Wijaya E , Lenaerts C , Maricot S , Hastanin J , Habraken S , Vilcot J P , Boukherroub R , Szunerits S . Surface plasmon resonance-based biosensors: From the development of different spr structures to novel surface functionalization strategies [J ] . Current Opinion in Solid State & Materials Science , 2011 , 15 ( 5 ): 208 - 224 .

Chen Y , Fan H , Li R , Zhang H , Zhou R , Liu G L , Sun C , Huang L . Free electron density gradients enhanced biosensor for ultrasensitive and accurate affinity assessment of the immunotherapy drugs [J ] . Advanced Science , 2024 , 11 ( 46 ): 2404559 .

Yao T. , Li T. , Qin Y. , Wang J. , Zhao Z. , Gu X. , & She Y. Recent Advances in Application of Surface Plasmon Resonance Sensors Based on Molecular Imprinted Polymers in Food Safety Detection [J ] 姚婷, 李腾飞, 秦玉昌, 王静, 赵祯, 谷旭, 佘永新. 分子印迹表面等离子共振传感器在食品安全检测中的最新研究进展 [J ] . 分析测试学报), 2015 , 34 ( 2 ): 237 - 244 .

Fan H , Li R , Chen Y , Zhang H , Zeng S , Ji W , Hu W , Yin S , Li Y , Liu G L , Huang L . Flexible nanoplasmonic sensor for multiplexed and rapid quantitative food safety analysis with a thousand-times sensitivity improvement [J ] . Biosensors and Bioelectronics , 2024 , 248 : 115974 .

Zhou T , Ji W , Fan H , Zhang L , Wan X , Fan Z , Liu G L , Peng Q , Huang L . A metasurface plasmonic analysis platform combined with gold nanoparticles for ultrasensitive quantitative detection of small molecules [J ] . Biosensors , 2023 , 13 ( 7 ): 681 .

Chen M , Xie Y , Li M . Molecular-sieving label-free surface-enhanced raman spectroscopy for sensitive detection of trace small-molecule biomarkers in clinical samples [J ] . Nano Letters , 2024 , 24 ( 37 ): 11520 - 11528 .

Li R , Fan H , Zhou H , Chen Y , Yu Q , Hu W , Liu G L , Huang L . Nanozyme-catalyzed metasurface plasmon sensor-based portable ultrasensitive optical quantification platform for cancer biomarker screening [J ] . Advanced Science , 2023 , 10 ( 24 ): 2301658 .

Li R , Fan H , Chen Y , Yin S , Liu G L , Li Y , Huang L . Mxene−graphene oxide heterostructured films for enhanced metasurface plasmonic biosensing in continuous glucose monitoring [J ] . Advanced Science , 2025 , 12 ( 4 ): 2410376 .

Zhao Y , Li R , Liu Z , Zhou H , Yang J , Zhang S , Huang L , Liu G L , Zhang Q , Jin M . Rapid, multispecies detection of sars-cov-2 antibodies via a meta-surface plasmon resonance biosensor [J ] . Transboundary and Emerging Diseases , 2024 , 2024 ( 1 ): 9350822 .

Fan H , Huang L , Li R , Chen M , Huang J , Xu H , Hu W , Liu G L . A nanoplasmonic portable molecular interaction platform for high‐throughput drug screening [J ] . Advanced Functional Materials , 2022 , 32 .

Wekalao J , Patel S K , Balamurugan A M , Al-Zahrani F A . Graphene-enabled multiresonator metasurfaces for ultrasensitive surface plasmon resonance detection of waterborne bacteria across multiple frequencies with machine learning optimization [J ] . Plasmonics , 2024 , 10.1007/s11468-024-02645-2( http://dx.doi.org/10.1007/s11468-024-02645-2(

Zhang W , Dang T , Li Y , Liang J , Xu H , Liu G L , Hu W . Digital plasmonic immunosorbent assay for dynamic imaging detection of protein binding [J ] . Sensors and Actuators B: Chemical , 2021 , 348 : 130711 .

Dang T , Hu W , Zhang W , Song Z , Wang Y , Chen M , Xu H , Liu G L . Protein binding kinetics quantification via coupled plasmonic-photonic resonance nanosensors in generic microplate reader [J ] . Biosensors and Bioelectronics , 2019 , 142 : 111494 .

Song L , Wang Z , Zhou D , Nand A , Li S , Guo B , Wang Y , Cheng Z , Zhou W , Zheng Z , Zhu J . Waveguide coupled surface plasmon resonance imaging measurement and high-throughput analysis of bio-interaction [J ] . Sensors and Actuators B: Chemical , 2013 , 181 : 652 - 660 .

Li R , Zhou Y , Chen Y , Zhou L , Yang Y , Xiao J , Liu G L , Wang J , Huang L , Li Y . High-electron-mobility mxene-enhanced metasurface biosensors integrated with microfluidics for real-time multifunctional monitoring [J ] .

Huang L , Ding L , Zhou J , Chen S , Chen F , Zhao C , Xu J , Hu W , Ji J , Xu H . One-step rapid quantification of sars-cov-2 virus particles via low-cost nanoplasmonic sensors in generic microplate reader and point-of-care device [J ] . Biosensors & bioelectronics , 2021 , 171 : 112685 .

Kang S , Yang M , He S , Wang Y , Chen X , Chen Y-Q , Hong Z , Liu J , Jiang G , Chen Q , Zhou Z , Zhou Z , Huang Z , Huang X , He H , Zheng W , Liao H-X , Xiao F , Shan H , Chen S . A sars-cov-2 antibody curbs viral nucleocapsid protein-induced complement hyperactivation [J ] . Nature Communications , 2021 , 12 ( 1 ): 2697 .

Wang Z , Hu N , Zhou Y , Shi N , Shen B , Luo L , Feng J . Structure-guided affinity maturation of a novel human antibody targeting the sars-cov-2 nucleocapsid protein [J ] . Scientific Reports , 2022 , 12 ( 1 ): 8469 .

Ye Y-m , Cho Y S , Lee S Y , Park J-w , Choi J-H , Kim S-H , Lee S Y , Shim Y , Song E , Sim S , Pak K C , Cho H-J , Kim S K , Jang M H , Park H-s . Safety, tolerability, pharmacokinetics, and pharmacodynamics of yh35324, a novel long-acting high-affinity igetrap-fc fusion protein, in patients with chronic spontaneous urticaria refractory to h1 antihistamines [J ] . Journal of Allergy and Clinical Immunology , 2025 , 155 ( 2 ): AB212 .

Hu M , Wei S , Zhou W , Wang P . Research progress on neonatal fc receptor and its application [J ] .

Zhejiang Da Xue Xue Bao Yi Xue Ban(胡茫莎 , 韦树丽 , 周武源 , 王苹莉 . 新生儿Fc受体基础研究和临床应用进展 [J ] . 浙江大学学报(医学版)) , 2021 , 50 ( 4 ): 537 - 544 .

Mandrup O A , Ong S C , Lykkemark S , Dinesen A , Rudnik-Jansen I , Dagnæs-Hansen N F , Andersen J T , Alvarez-Vallina L , Howard K A . Programmable half-life and anti-tumour effects of bispecific t-cell engager-albumin fusions with tuned fcrn affinity [J ] . Commun Biol , 2021 , 4 ( 1 ): 310 .

Zhou J , Johnson J E , Ghetie V , Ober R J , Sally Ward E . Generation of mutated variants of the human form of the mhc class i-related receptor, fcrn, with increased affinity for mouse immunoglobulin g [J ] . Journal of Molecular Biology , 2003 , 332 ( 4 ): 901 - 913 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

基于凝胶电泳芯片的DNA定量分析方法的研究

人血清白蛋白与氨基酸对映异构体的差异性识别研究

微流控芯片技术-斑马鱼模型在药物筛选研究中的进展

药物筛选新技术及其应用进展