最新刊期
卷 37 ， 期 11 ， 2018
- 摘要：A liquid chromatography-mass spectrometric method was established for the identification of the species origin of collagen based on peptide markers.The sample was determined by nano electrospray ionization quadrupole time of flight mass spectrometry in positive mode to obtain peptide mass spectrometry information after protein extraction，reduction，alkylation，tryptic digestion and followed by the separation on an Eksigent C18 column（75 μm×150 mm，3 μm） by gradient elution with water-acetonitrile（98∶2） containing 01%formic acid and acetonitrile-water（98∶2） containing 01%formic acid as mobile phases.Potential peptide markers were screened by data analysis with ProteinPilotTM and blast.Then the digested sample was separated on an Eclipse Plus C18 column（21 mm×100 mm，18 μm） by gradient elution with acetonitrile-water containing 01%formic acid as mobile phase.The specificity of peptide markers was further confirmed by electrospray ionization quadrupole/linear ion trap tandem mass spectrometry in positive mode under multiple reaction monitoring information dependent acquisition enhanced product ion（MRM-IDA-EPI） scanning mode.Finally，three pig collagen peptide markers，four bovine collagen peptide markers and one sheep collagen peptide marker were screened and identified.These peptide markers have a good heat resistance，and could provide a specific，accurate and reliable method for the identification on species origin of collagen.关键词：peptide marker;collagen;liquid chromatography-mass spectrometry;species origin
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发布时间： - 摘要：Based on the experiments of artificial cultivation of Suaeda salsas in coastal wetland of Qinhuangdao,the enrichment characteristics of metal elements with Suaeda salsas were studied through analyzing the contents and changes of different metal elements in wetland soil.The results showed that the contents of Fe,Mn,Cr,Pb and Zn in sediments were relatively high,with their average contents of 8 210.94,110.04,8.78,8.25,10.95 mg/kg,respectively,while the content of Cd was the lowest,with an average value of 0.022 mg/kg.The variation degree for the same metal in the sediment of the wetland was relatively small,and the distribution was more uniform.The contents of heavy metals in Suaeda salsas were different according to different sampling sites,but there was a positive correlation for them with the distribution characteristics of metals in the sediments from each station.The contents of Fe,Mn,Zn and Cu were relatively high,and the contents of Fe,Mn,Ni,Cu,As,Cd and Pb in the roots of Suaeda salsas were higher than those in stems and leaves.The content of Cr was the highest in the stems,and those of Zn,Mo were the highest in the leaves.The roots,stems and leaves of Suaeda salsas had the best enrichment effect on Cd,followed by Mo and Cu,and Suaeda salsas had a poorer enrichment ability to Fe,Mn,Zn,As and Pb,which showed that there were differences for Suaeda salsa in the enrichment and removal rates of different metal elements in the sediment.Study of translocation factors showed that Mo,Zn,Cd and Mn were transferred from the roots to the leaves,while Fe,Ni,Pb,As and Cu were immobilized at the roots of Suaeda salsas.This study could provide a theoretical basis for the remediation of heavy metal pollution in wetlands with Suaeda salsas.关键词：Suaeda salsas;heavy metal;wetland restoration;bio-concentration
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发布时间： - 摘要：A comprehensive analytical method based on ultra fast liquid chromatography tandem triple quadrupole mass spectrometry（UFLC-QTRAP-MS/MS） was developed for the simultaneous determination of 17 ginsenosides in different stored samples of Ginseng Radixet Rhizoma Rubra.Under the optimized chromatographic conditions，good separation for seventeen target compounds were obtained on a SynergiTM Hydro-RP 100 A column（100 mm×2.0 mm，2.5 μm） using 0.1%aqueous formic acid-acetonitrile as mobile phase at a flow rate of 0.4 mL/min.The target compounds were analyzed under multiple reaction monitoring（MRM） mode with an ESI source operating in negative ion mode，hierarchical cluster analysis（HCA） and principal component analysis（PCA） were used for data processing.The results showed that there existed good linearities for the 17 components in the certain concentration ranges，with their correlation coefficients larger than 0.999 0.The precision，repeatability and stability of the method were all satisfactory.The spiked recoveries for the compounds were between 96.4%and 103%，with relative standard deviations less than 5%.The results showed that the optimum storage conditions for Ginseng Radixet Rhizoma Rubra were as follows：storing it with a carton at -20 ℃，protected from light.The proposed method is useful for assessment and control on the quality of Ginseng Radix et Rhizoma Rubra，and provides a scientific basis for choosing the most suitable storage condition of Ginseng Radix et Rhizoma Rubra.关键词：Ginseng Radix et Rhizoma Rubra;ultra fast liquid chromatography tandem triple quadrupole mass spectrometry（UFLC-QTRAP-MS/MS）;ginsenosides;storage conditions;principal component analysis（PCA）;hierarchical cluster analysis（HCA）
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发布时间： - 摘要：An analytical method was established for the simultaneous screening and determination of 112 prohibited drugs,including quinolones,glucocorticoids,nitroimidazoles,tetracyclines,macrolides,sulfonamides,cephems and androgens in cosmetics by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The sample was extracted with 0.2% formic acid-acetonitrile,and then purified by QuEChERS method.The extracts were separated on an Agilent ZorbaxEclipsePlus C18(3.0 mm×150 mm,1.8 μm) column with 0.2% formic acid-acetonitrile solution containing 0.2% formic acid as mobile phase by gradient elution,and analyzed by LC-MS/MS under dynamic multiple reaction monitoring(DMRM) mode.Results showed that the limits of quantitation(LOQ,S/N=10) for 112 target compounds in cosmetics were in the range of 2.0-10.0 μg/kg.The mean recoveries for 112 prohibited drugs were in the range of 82.0%-108% with their RSDs of 2.0%-7.9%.The method has the advantages of simplicity,rapidness and high efficiency,and could be applied in the screening and confirmation on 112 prohibited drugs in cosmetics in routine analysis.
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发布时间： - 摘要：A reliable,efficient and simple method was developed for the determination of N glycans in glycoproteins based on the enzymatic labeling and the acetone precipitated glycopeptides enrichment.Taking sialylglycopeptide(SGP) for instance,a 5 fold volume of acetone was confirmed to be appropriate for the selective precipitation of SGP by comparing the enriching effects of adding volumes of acetone on SGP.To confirm the selectivity of acetone precipitation for N-glycopeptides,tryptic digest from bovine ribonuclease B(RNase B) was treated with acetone,following by chemoenzymatic labeling and a transglycosylation reaction utilizing Endo-M N175Q and PDPZ-Boc-Asn-GlcNAc as acceptor,and the PDPZ Boc Asn GlcNAc N-glycans were obtained.The chromatographic separation was conducted on a YMC C18 column using water containing 10 mmol/L HCOONH4-acetonitrile as mobile phase by gradient elution.The analysis was performed by ESI-MS in positive mode.Five high mannose types of N-glycans in RNase B were detected.Results showed that N-glycosylated peptides could be enriched in the precipitate by adding acetone,and the chemoenzymatic labeling was performed in the presence of the glycopeptides and PDPZ-Boc-Asn-GlcNAc as enzymic catalytic substrates.The method was successful in acquiring the comprehensive MS data for five complex types of glycans in fetuin.Therefore,it is a powerful tool for the determination of N-glycans in glycoproteins.
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发布时间： - 摘要：In order to evaluate the safety of dimethomorph and cyazofamid in cucumber and soil，a high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS） was developed for the simultaneous detection of dimethomorph，cyazofamid and its metabolite，4-chloro-5-p-tolylimidazole-2-carbonitrile（CCIM） in cucumber and soil.Samples were extracted with acetonitrile，cleaned up with PSA and C18，and then analyzed by HPLC-MS/MS in multiple reaction monitoring（MRM） mode with the matrix matched calibrations method.There existed good linearities for All analytes in the range of 0.01- 0.5 mg/L with their correlation coefficients（r） more than 0.994 0.At three spiked levels of 0.05，0.1 and 0.5 mg/kg，the recoveries for dimethomorph，cyazofamid and CCIM in cucumber and soil were in the range of 78%-105%with relative standard deviations（RSDs） of 1.3%-14.8%.The limit of quantitation was 0.05 mg/kg.Residues and dissipation of dimethomorph and cyazofamid in cucumber and soil were investigated.The half lives of dimethomorph in cucumber and soil were 1.2-2.1 d and 3.0-9.6 d，respectively，and the half lives of cyazofamid and CCIM in cucumber and soil were 0.9-2.3 d and 1.8-6.2 d，respectively.The method is simple，rapid，sensitive and accurate，and could meet the requirements for detection of dimethomorph，cyazofamid and CCIM residues in cucumber and soil.关键词：dimethomorph;cyazofamid;metabolite;cucumber;soil;high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）
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发布时间： - 摘要：A high resolution hybrid linear ion trap time of flight mass spectrometer（LIT-TOF MS） was constructed，which is based on the time of flight mass spectrometry，linear ion trap，atmospheric pressure ionization source and other core technologies.The performance of the instrument was characterized with an electric spraying ion source.The resolution of LIT-TOF MS was over 12 000（Reserpine，m/z 609），the mass range was 33-1 922 amu，and the sensitivity was 1 μg/L.It was also demonstrated that MS3 could be readily performed in the LIT.The qualitative and quantitative analyses on amino acids were carried out by the LIT-TOF MS，and results showed that it could be used in the real time，on line and high throughput determination of contents and variations of amino acids in biological complex samples.Besides，the instrument，combined with a variety of atmospheric pressure ionization techniques，could be applicable for determination in fields of medicine，environment and foods，etc.关键词：electrospray ion source;linear ion trap;time of flight mass spectrometer;hybrid mass spectrometer
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发布时间： - 摘要：A method was established for the simultaneous determination of carnosine and anserine in animal derived food extracts by ultrahigh performance liquid chromatography-electrospray ionization triple quadrupole tandem mass spectrometry.The separation on the analytes was performed by an ultrahigh performance liquid separation system with an Inertsil Amide HP(2.1 mm×100 mm,3 μm) column using 0.1% acetic acid and acetonitrile as mobile phases by gradient elution,and the external standard method of peak area was used for quantitation.There existed good linear relationships for the peak areas of carnosine and anserine in the concentration range of 1.95-500 μg/L,with their correlation coefficients(r) larger than 0998.The detection limits for carnosine and anserine were 0.12 μg/L and 0.47 μg/L,respectively.The low concentration oligopeptide raw material was used as the substrate,and the quality control of 2,20,and 200 μg/L was spiked.The recoveries for carnosine and anserine at three spiked levels of 2,20 and 200 μg/L were in the ranges of 993%-109% and 100%-113%,with their relative standard deviations(RSD,n=6) of 0.98%-1.1% and 1.0%-1.1%,respectively.With the advantages of simple pretreatment,rapidness,good reproducibility and high precision,the method is applicable for the simultaneous detection of carnosine and anserine in animal derived food extracts.关键词：ultra high performance liquid chromatography-tandem mass spectrometry(UHPLC-ESI-MS/MS);carnosine;anserine;animal derived food extract
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发布时间： - 摘要：A noval method was established for the detection of four alternaria mycotoxins,iealternariol,alternariol monomethyl ether,tenuazonic acid and tentoxin in jujube by ultrahigh performance liquid chromatography-tandem mass spectrometry.The sample was extracted with 2.0% formic acid-acetonitrile by sonication and auxiliarily salted out with 0.2 g NaCl.Four alternaria mycotoxins in jujube were separated on a BEH C18 column in 6 min,and dectected in multiple reaction monitoring mode(MRM).The calibration curves for 4 alternaria mycotoxins showed good linearities in the ranges of 1.0-100 μg/L for alternariol and 10-1 000 μg/L for the other three toxins,with their correlation coefficients larger than 0.990 and the detection limits of 0.5-12.4 μg/kg.The spiked recoveries for 4 toxins in blank matrix were between 92.8% and 116% with the relative standard deviations of 0.5%-11.2%,which could meet the requirements for detection of alternaria mycotoxin in jujube.Moreover,the permitted daily exposures(PDE) for adults against 4 types of toxins were obtained to be 19.30-25.65 μg/d based on the calculation of the toxicological software theory.关键词：ultrahigh performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS);alternaria mycotoxins;jujube;risk assessment
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发布时间： - 摘要：A method of liquid chromatography mass spectrometry（LC-MS） was used to analyze the chemical weapon related compounds in the aqueous decontamination effluent samples of the 42nd Official Organization for the Prohibition Chemical Weapons（OPCW） Proficiency Test.Firstly，the blank sample，control sample and simulated sample were distinguished by HPLC-Q-TOF MS from the three aqueous decontamination effluent samples，then three chemical weapon related compounds were preliminarily found out in simulated sample.Finally，2-hydroxyethyl vinyl sulfide，divinyl sulfone and 1,2-bis（2-hydroxyethylthio）ethane in the simulated sample were identified simultaneously by UPLC-QQQ MS.The buffer agents，fragmentor voltages and fragmentation pathway were discussed adequately.The results obtained were in accordance with the list of spiking chemicals provided by the laboratory of OPCW.The method could provide a reference for the analysis on the decontamination products of sulfur mustard.关键词：liquid chromatography-mass spectrometry（LC-MS）;chemical weapon related compounds;aqueous decontamination effluent
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发布时间： - 摘要：A head space/gas chromatography-mass spectrometric(HS/GC-MS) method was established for the determination of 5-ethylidene-2-norbornene migration in food packaging materials.Water,4% aqueous acetic acid solution,50% aqueous ethanol solution and olive oil were chosen as food stimulant.Samples were soaked with the food stimulant,followed by injection after gas-liquid equilibrium and separation with a DB-5 MS capillary(30 m×0.25 mm×0.25 μm) column.The target was determined in selective ion mode and quantified by external standard method.The pretreatment methods(solvent extraction-direct injection and gas-liquid balance head space injection),headspace balance temperature,and headspace balance time were optimized.Under the optimal conditions,there was a good linearity for 5 ethylidene 2-norbornene between its peak areas and concentrations in the range of 0.1-10 mg/L with correlation coefficients(r) more than 0.999.The spiked recoveries for 5-ethylidene-2-norbornene in fruits at three spiked levels of 0.1,1.0,10 mg/L ranged from 92.0% to 108%,with relative standard deviations(RSDs,n=5) of 0.9%-4.0%.The limits of detection(LOD) of this method were 0.01 mg/kg,and the limits of quantitation(LOQ) were 0.03 mg/kg.With the advantages of accuracy,reliability and high sensitivity,this method was suitable for the determination of 5 ethylidene 2-norbornene migration in food packaging materials.关键词：5-ethylidene-2-norbornene(ENB);food simulant;food packaging materials;head space/gas chromatography-mass spectrometry(HS/GC-MS)
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发布时间： - 摘要：A method was developed for the simultaneous determination of 11 phenoxy acid herbicides in infant cereal food by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The samples were extracted with acetonitrile containing 2% formic acid,then purified with N propyl ethylenediamine(PSA) and anhydrous magnesium sulfate(MgSO4).The target compounds were separated on a ZORBAX Eclipse XDB-C18 column(4.6 mm×50 mm,1.8 μm),and detected by HPLC-MS/MS with electrospray ionization(ESI) in negative ion mode under multiple reaction monitoring(MRM) mode,and quantified by the external standard method.The calibration curves for 11 phenoxy acid herbicides were linear in the range of 0.5-200 μg/L with their correlation coefficients(r2) larger than 0999.The average recoveries at 3 spiked levels were in the range of 77.4%-999%,with relative standard deviations(RSDs,n=7) of 1.5%-8.0%.The limits of detection(S/N=3) and quantitation(S/N=10) of the method were 0.1-2.4 μg/kg and 0.4-8.1 μg/kg,respectively.Owing to the advantages of simple operation,high accuracy and sensitivity,this method is suitable for the rapid and simultaneous detection of 11 phenoxy acid herbicides in infant cereal food.关键词：high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS);phenoxy acid herbicides;QuEChERS;infant cereal food
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发布时间： - 摘要：An analytical method was developed for the simultaneous detection of flupyradifurone and its three metabolites ie.difluoroacetic acid（DFA），6-chloronicotinic acid（6-CNA） and difluoroethylaminofuranone（DFEA） in citruses by ultrahigh performance liquid chromatography tandem mass spectrometry（UPLC-MS/MS）.Citrus samples were extracted with acetonitrile，and cleaned up by dispersive solid phase extraction（PSA）.The analytes were detected in multiple reaction monitoring（MRM） mode under alternate positive/negative electrospray ionization（ESI+/ESI- ） mode，and quantified by the external standard method.The results showed that there were good linearities for flupyradifurone and its metabolites in their respective concentration ranges with correlation coefficients of 0.997 9-0.999 6.The limits of detection were 0.001 mg/L.The average recoveries for the analytes in the whole citrus fruit and fruit pulp at spiked levels of 0.05，0.3 and 2 mg/kg ranged from 71.9%to 106%with relative standard deviations（RSDs） of 0.8%-9.1%.The average recoveries of the analytes in citrus fruit peel at spiked levels of 0.1，0.3 and 3 mg/kg ranged from 77.9%to 100%with relative standard deviations（RSDs） of 1.2%-6.5%.The limits of quantitation（LOQs） for flupyradifurone and its metabolites in the whole citrus fruit，fruit pulp and peel were 0.05，0.05 and 0.1 mg/kg，respectively.The method is simple，stable，rapid and sensitive，and is suitable for the determination of flupyradifurone and its metabolites in citrus samples.关键词：pesticide residue;flupyradifurone;metabolites;citrus;ultrahigh performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）
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发布时间： - 摘要：A novel gas chromatography-mass spectrometric(GC-MS) method coupled with purge and trap was developed for the simultaneous determination of nine neutral volatile polyfluorinated iodine alkanes(PFIs) in water samples.Several P&T parameters such as type of traps,purge time,purge temperature,desorbing time and ionic strength were optimized.Under the optimal conditons,the calibration curves for the nine PFIs showed good linear relationships(r2≥0.996) in the concentration range of 0.1-100 μg·L-1.The limits of detection(LOD) ranged from 5.6 ng·L-1 to 15 ng·L-1,and the limits of quantitation were from 17 ng·L-1 to 36 ng·L-1.The spiked recoveries for the analytes in water samples were in the range of 75.7%-118% with relative standard deviations(RSDs,n=7) of 2.2%-83%.The method was applied in the detection of volatile PFIs in water samples from the vicinity of a fluorochemical manufacturing plant,and nine PFIs were detected,with concentrations of 21.3-469 ng·L-1.关键词：polyfluorinated iodine alkanes;purge and trap;gas chromatography-mass spectrometry(GC-MS);water
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发布时间： - 摘要：A simple,sensitive and reliable analytical method was developed for the simultaneous determination of 16 cephalosporins in formulated feeds by ultra high performance liquid chromatography-positive electrospray ionization tandem mass spectrometry.Samples were extracted with acetonitrile-water(40∶60) solution,and directly diluted using 0.1% formic acid solution after centrifugation,then analyzed by LC-MS/MS.The samples were separated on an Acquity UPLC BEH C18 column with a mixture of 0.1% formic acid solution and methanol as mobile phase by gradient elution,and detected in multiple reaction monitoring mode.The matrix matched external standard method was applied for the quantitative analysis.There are good linear relationships for sixteen cephalosporins in the certain concentration ranges with their correlation coefficients more than 0.999.The limits of detection for sixteen cephalosporins were in the range of 1.5-15 μg/kg,and the limits of quantitation were 5.0-50 μg/kg.The recoveries for the analytes in feeds at spiked levels of 10-2 500 μg/kg were in the range of 88.4%-93.6%,with relative standard deviations of 0.8%-4.7%.The method is suitable for the determination of sixteen cephalosporins in formulated feeds due to its simplicity and reliability.关键词：formulated feeds;cephalosporins;liquid chromatography with tandem mass spectrometry;concentration
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发布时间： - 摘要：A method based on liquid chromatography-tandem mass spectrometry(LC-MS/MS) with solid supported liquid-liquid extraction was developed for the determination of cyclosporin A in human whole blood.The whole blood sample was loaded on the solid supported liquid-liquid extraction column after protein precipitation,and then eluted with methyl tert butyl ether.The extract was injected into a Shim pack XR-ODS(75 mm×3.0 mm i.d.,2.2 μm) column for separation,followed by detection of mass spectrometry in multiple reaction monitoring mode via an electrospray ionization interface.The quantitation was achieved using cyclosporin D as the internal standard.There was a good linearity for the method toward cyclosporin A in the range of 1.5-500 μg/L with a correlation coefficient(r) of 0.998.The limits of detection and quantitation were down to 0.5 μg/L and 1.5 μg/L,respectively.The average recoveries for the analyte at three spiked levels ranged from 78.6% to 83.5% with the intra day and inter day relative standard deviations of 3.1%-5.6% and 4.5%-8.3%,respectively.This method is suitable for the determination of cyclosporin A in whole blood sample due to its advantages of simplicity,rapidness,accuracy and reliability.关键词：cyclosporin A;whole blood;solid supported liquid-liquid extraction;liquid chromatography-tandem mass spectrometry(LC-MS/MS)
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发布时间： - 摘要：An ultrahigh performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS)method was established for the determination of flufenacet in rats urine and feces.The urine and feces samples were extracted with acetonitrile.Chromatographic separation of the target compound was performed on a C18 column(50 mm×46 mm,5 μm) using ultrapure water containing 01% formic acid and acetonitrile as mobile phases at a flow rate of 06 mL·min-1.The detection of flufenacet was carried out by MS with electrospray ionization in positive ionization under multiple reaction monitoring mode.There were good linear relationships for flufenacet in rats’ urine and feces over the concentration ranges of 010-100 mg/L and 025-500 mg/L,with their correlation coefficients(r) larger than 099.The limits of quantitation for flufenacet in urine and feces were 010 and 025 mg/L,respectively.The intra RSDs and inter RSDs for the QC sampes were not more than 99%.The stability for flufenacet in urine ranged from 937% to 108%,and the recoveries and matrix effects were in the ranges of 970%-988% and 988%-107%,respectively.All the results met the requirements for bioanalytical method validation.The excretion kinetics in rats was also investigated after oral administration of flufenacet at a dosage of 400 mg/kg by body weight.The total accumulated excretion rate for flufenacet in urine and feces was 1262% in 144 h,in which the excretion percentages for urine and feces were 112% and 1013%,respectively.Results indicated that flufenacet was mainly excreted through feces.This method has the advantages of high sensitivity,selectivity and accuracy,and could be applied in the analysis of flufenacet in rats urine and feces.关键词：flufenacet;ultrahigh performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS);rats;excretion
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发布时间： - 摘要：Matrix assisted laser desorption/ionization time of flight mass spectrometry（MALDI-TOF MS） is a new kind of soft ionization bio mass spectrometry，which has been rapidly developed in the past decade.It plays an important role in the analysis of small molecule compounds.The selection of matrix has always been the focus of researchers′ attention.At present，a variety of new MALDI-TOF MS matrixes have been developed，which could be divided into two major categories：nanomaterials and new organic compounds.The improvement of matrixes properties makes MALDI-TOF MS work with higher accuracy and sensitivity，smaller background noise，and cleaner spectra.Nevertheless，many reports are still at the research stage，and the traditional organic matrixes still could not be replaced at this stage.In this paper，the research progress of the new matrixes is sorted out，the advantages，characteristics and applicable objects of different types of matrixes are summarized.and the research future of matrixes for MALDI-TOF MS is prospected.关键词：matrix assisted laser desorption/ionization time of flight mass spectrometry（MALDI-TOF MS）;small molecule compounds;novel matrixes;review
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发布时间： - 摘要：Lipids play an essential role in cells and tissues as they are basic molecules responsible for the functions including structure supporting,energy storage and signal transduction.Although traditional analytical methods in lipidomics could provide a relative high selective and sensitive identification for lipids,the diversity and similarity in lipid structures still pose a great challenge toward the global analysis of lipids in biological samples.Ion mobility spectrometry（IMS） could separate the ions in buffer gas or electric field based on their mobility characteristics.The combination of IMS and traditional analysis approaches for lipids could not only attribute to the separation of sophisticated lipids,but also identify the lipid isomers,and thus improving the selectivity and resolution of lipid analysis.In this paper,various kinds of ion mobility mass spectrometry and their applications in lipids analysis were summarized,and their development prospects in future were expected as well.关键词：lipids;ion mobility spectrometry（IMS）;ion mobility mass spectrometry;isomer
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发布时间： - 摘要：Glycerophospholipids are the main components of all biological cell membranes,which play a very important physiological role in many life activities,including serving as an extracellular mediator in induction of cellular proliferation,involving in the development of nervous and vascular systems,intracellular signaling,and suppression of apoptosis.It is a great challenge for the identification and quantitative analysis of phospholipids due to the variety of glycerophospholipid species,their occurrence at low concentrations in biological samples,matrix effects and the lack of easily ionized groups in their chemical structures.Effective approach has been employed to improve their ionization efficiency,chromatographic resolution,and significantly increase the sensitivity of mass spectrometry(MS) detection by modifying the chemical structures of glycerophospholipids through derivatization technique.The integration of MS with derivatization has been widely used in proteomics,glyconics,metabolite analysis and many other applications.In recent years,this strategy has been gradually applied in lipidomics.An overview on derivatization in MS-based strategy for lipidomic analysis over the last decade is presented in this review,and glycerophospholipids which are relatively important lipids in biological systems are mainly focused.关键词：derivatization;glycerophospholipid;mass spectrometry(MS);lipidomics
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