最新刊期
卷 34 ， 期 3 ， 2015
- 摘要：Microfluidic technologies,arising from multi disciplinary intersection,has become an indispensable platform in natural science because of its characteristics of miniaturization,integration and high throughput.In this review,the recent applications of microfluidic technologies in life science research were outlined.The applications were divided into four categories according to the scale of the analyzed objects:biomacromolecule analysis,cell based analysis,organs on chips,and studies on small model organisms.And the development prospects of this microfluidic technologies were proposed.关键词：microfluidic chip;biomacromolecule;cell;organs;organism;review
- 2256
- |
- 546
- |
- 0
发布时间：2023-05-18 - 摘要：Paper-based microfluidics stand out as a new class of microfluidic technology,and present distinguishing features such as low cost,ease of use,portability,and low reagent consumption compared with the conventional microfluidic devices.In this paper,the development of μPADs was first introduced,and the common fabrication techniques were presented.Then the methods for quantitative analysis on μPADs were summarized including colorimetry,fluorescence,chemiluminescence,electrochemiluminescence and electrochemistry with their applications in clinical diagnostics,environmental monitoring as well as food quality control.Finally,the potential and future outlooks of μPADs were discussed.关键词：paper-based microfluidics;fabrication;detection;application;review
- 2936
- |
- 6
- |
- 9
发布时间：2023-05-18 - 摘要：The microfluidic platform combined with surface enhanced Raman scattering（SERS） spectrum analytical method has attracted great attention in the field of biochemical assay because its advantages such as simple sample preparation,nondestructive testing,better identification and applicability in water environment detection.The key points of establishing the analytical method based on the microfluidic SERS chip are SERS chip design and fabrication,which also are the core issues for improving the detection sensitivity and reproducibility.Based on the demonstration of the basic configuration and function of microfluidic SERS chip,the fabrication of SERS substrate in a microfluidic SERS chip and the SERS effects of the chip are mainly summarized in this paper.The SERS substrates fabricated by Micro-Electro-mechanical-System（MEMS） technology are of the characters such as nanometer particle size controlled,easy integration with microfluidic devices and limitation of the materials species.Meanwhile，the SERS substrates fabricated by physical or chemical methods，such as chemical deposition and self assembly,have the advantages of easy materials expansion,low cost and flexible combination with microchannels.The microfluidic SERS chip and the analytical methods display a great potential in the field of biochemical detection,especially in the bacteria monitoring.关键词：microfluidic SERS chip;SERS substrate;bacteria detection;review
- 2253
- |
- 577
- |
- 5
发布时间：2023-05-18 - 摘要：The specific functions of biomacromolecules such as proteins or nucleic acid highly depend on their three-dimensional structures.Folding kinetics investigation could reveal the mechanism of how the biomolecules transform from the free linear structure into the functional high-level structure,thus much attention has been paid to this scientific field recently.The biomolecular folding events often occur in the time scale of millisecond or even microsecond,which means that it is necessary to initiate the reaction before that time limit.Ultra-fast micromixer could initiate reactions in a short period of time and has been recognized as an attractive tool to analyze the kinetics of fast reactions.The research progresses of ultra-fast micromixers and their applications in the folding kinetics of biomacromolecules are summarized in this review.关键词：biomacromolecule;folding kinetics;microfluidics;micromixer
- 2269
- |
- 519
- |
- 0
发布时间：2023-05-18 - 摘要：Microfluidic chip electrophoresis could be an ideal candidate for rapid on site analysis associated with food safety and environmental pollutions due to its advantages of low sample/reagent consumption,rapid analysis,high separation performance and easy miniaturization.This review covers the typical applications of this technique in areas of food safety and environmental analysis,including detection of harmful residues and illegal additives in foods,and determination of toxic inorganic ions and organic pollutants in environmental samples.The factors that limit the applications of this technique in the real-world analytical practice are discussed,and the potential ways to solve these problems are proposed.关键词：microfluidics;electrophoresis;food safety;environmental analysis;review
- 2302
- |
- 488
- |
- 6
发布时间：2023-05-18 - 摘要：Sample pretreatment is one of the bottlenecks of microfluidic chip.In recent years,monolithic material has been developed as a new chromatographic packing in the field of chromatography.It has been employed in microfluidic chip because of its advantages of uniform structure,fast mass transfer,good permeability,and simple preparation process.This review summarizes recent developments of the application of monolithic column in microfluidic chip.80 references are cited.关键词：monolithic column;preconcentration;microfluidic chip;application;review
- 1967
- |
- 629
- |
- 1
发布时间：2023-05-18 - 摘要：Zebrafish,as an important model organism,offers more means for researching in human diseases.However,the conventional multi-well plates and standard liquid handling techniques procedures generally used in the zebrafish research are limited by the low throughput,low automatization,and imprecise delivery of external stimulation.Microfluidics platform,as an emerging technology is attractive to many researchers with marvelous advantages.In this review,the disease models of zebrafish were summarized,and the research and process of zebrafish or their embryos with the microfluidic platform were introduced in detail.Furthermore,the trends of future development were also discussed.关键词：microfluidic;zebrafish;drug screening;review
- 2441
- |
- 487
- |
- 1
发布时间：2023-05-18 - 摘要：Microfluidic chip is a kind of technology manipulating fluids in micrometer scale with advantages of flexible integration of variety of cell technology,reducing sample consumption and etc.Raman spectroscopy is an important spectroscopic technique used widely in the fields of chemical,physical and biological sciences,and many other disciplines.Moreover,based on the surface of silver or gold nanoparticles,the enhanced Raman（SERS） technique has very high sensitivity that could be easily used for monitoring environmental pollutants and biological analysis of samples.This paper mainly summarizes the research progress of microfluidic chip in the field of surface-enhanced Raman spectra,including nanoparticle synthesis,chip design and common sensor types,and introduces its applications in life sciences and environmental monitoring,which reveals its broad application prospects.关键词：microfluidic chip;lab on a chip;surface-enhanced Raman;environmental pollutent;bioanalytical analysis;review
- 3096
- |
- 546
- |
- 6
发布时间：2023-05-18 - 摘要：In this paper,an application of microchip electrophoresis with chemiluminescence detection（MCE-CL） in single human red blood cell analysis was described.Human red blood cells were assayed to determine the intracellular content of hemoglobin（Hb）.The injection of individual cells,in-line lysed,and MCE separation were integrated onto a simple cross microfluidic chip.CL detection was based on the catalytic effects of hemoglobin on the luminol-hydrogen peroxide reaction.The MCE-CL detection had a linear calibration curve over the range of 2.0-90 pg injected with a correlation coefficient of 0.998 9 and a detection limit（S/N=3） of 0.8 pg.The content of Hb in individual human red blood cell was found in the range of 14-68 pg（n=19）.Compared to other methods,the MCE-CL method is simple and sensitive,and could be an attractive alternative for single cell analysis.关键词：microchip electrophoresis;chemiluminescence detection;single cell analysis;human red blood cells;hemoglobin
- 2294
- |
- 564
- |
- 0
发布时间：2023-05-18 - 摘要：A new method was used for the rapid screening and identification of free radical scavengers in the extracts of Salvia miltiorrhiza Bunge and Salvia prattiiHemsl., and the determination of the content of the analytes in the samples based on HPLC-DAD-ESI/MSn-DPPH was developed.The three compounds were identified to be caffeic acid, salviaflaside and rosmarinic through comprehensive analysis of HPLC spectra, MS spectra and literature data.The extraction effects of the hot reflux,ultrasonic assisted extraction and accelerated solvent extraction(ASE) were compared and the chromatographic conditions were optimized.Under the optimized conditions,three compounds could be effectively separated.Good linear relationships were obtained for three compounds in the concentration range of 1.7-35.3 μg/mL,with correlation coefficients of 0.999 5-0.999 8.The detection limits were in the range of 0.05-1.85 μg/mL,and the quantitation limits were in the range of 0.18-6.16 μg/mL.The average recoveries of three compounds were between 96.6% and 97.2%,with relative standard deviations of 1.1%-1.3%.The method was applied in the determination of three compounds contents in Salvia miltiorrhiza Bunge and Salvia prattiiHemsl.,with caffeic acid:0.303,0.254 mg/g; salviaflaside:1.019,1.401 mg/g;rosmarinic:17.279,8.104 mg/g.The method is convenient,fast,accurate and reproducibile.Otherwise,it gives a powerful tool for the rapid screening and identification of free radical scavenging compounds in complex natural products.关键词：Salvia miltiorrhiza Bunge;Salvia prattiiHemsl.;antioxidant activity compounds;on-line HPLC-DAD-ESI/MSn-DPPH
- 2101
- |
- 512
- |
- 8
发布时间：2023-05-18 - 摘要：Microfluidic evaporative light scattering detector（μELSD） coupled to pressurized capillary electrochromatography（pCEC） was applied in the separation and detection of six active components,e.g.platycodin.D,peimisine,oleanolic acid,imperialine,peimine and peiminine in Chuanbei Pipa Syrups.The simultaneous analysis of six active components by pCEC-μELSD was performed on a C18 capillary column（EP-150-15-3-C18） with mobile phase A:acetonitrile and mobile phase B:10 mmol?L-1 formic acid-triethylamine aqueous solution（pH 11.0） by gradient elution.The optimal conditions were as follows:column flow rate:1.02 μL?min-1,applied voltage:+10 kV,nebulizing gas flow rate:0.4 L?min-1 and evaporative temperature:40 ℃.Six active components in Chuanbei Pipa Syrups were successfully separated in 10 min with the excellent linearity of four orders of magnitude.The limit of detection（LOD） could reach up to the level of pg,and the recoveries were between 97.9% and 103.0%.The precision,repeatability and stability indicated a good reproducibility for the determination.The results also demonstrated the feasibility and practicality of pCEC-μELSD platform.The method could be used for the determination of the active ingredients in Chuanbei Pipa Syrups.关键词：microfluidic evaporative light scattering detector（μELSD）;pressurized capillary electrochromatography（pCEC）;fritillaria alkaloids;platycodin D;oleanolic acid
- 1983
- |
- 472
- |
- 4
发布时间：2023-05-18 - 摘要：A new molecular imprinted electrochemical sensor was developed for the detection of 3,5-xylyl methylcarbamate(XMC) by combination of graphene and gold nanoparticles.The molecularly imprinted polymer(MIP) was synthesized on the surface of grapheme and gold nanoparticles modified glassy carbon electrode by using XMC as template molecular,methyl acrylic acid(MAA) as functional monomer,and ethylene glycol maleic rosinate acrylate(EGMRA) as cross-linker.The morphology of the sensing membrane was characterized by scanning electron microscopy(SEM).The performance of the sensor was investigated by cyclic voltammetry(CV),differential pulse voltammetry(DPV) and electrochemical impedance spectroscopy(EIS).A linear relationship between oxidation peak current and XMC concentration was obtained over the range of 1.0×10-7-2.0×10-5 mol?L-1 with a correlation coefficient of 0.997 9 and a detection limit(S/N=3) of 1.5×10-8 mol?L-1．The selectivity experiments showed that the imprinting factor(β) of XMC imprinted film was 294 and the selectivity factors(α) of XMC compared to interference were all larger than 1.Although a minor interference was generated by XMC for its structure similar to metolcarb,the selectivity factor also reached to 2.39.The results indicated that the imprinted polymer films exhibited an excellent selectivity for XMC.The kinetics of recognition suggested that the process of the imprinted polymer films adsorbing XMC is Langmuir model and its kinetic rate constant is 73.05 s.The imprinted sensor was successfully used to determine XMC in vegetable samples with recoveries of 95.4% -108.0% by using the standard addition method.关键词：3,5-xylyl methylcarbamate;graphene doping gold nanoparticles;ethylene glycol maleic rosinate acrylate;molecularly imprinted electrochemical sensor;selective recognition
- 2697
- |
- 623
- |
- 4
发布时间：2023-05-18 - 摘要：A method for the determination of glyphosate(PMG) and aminomethylphosphonic acid(AMPA) residues in tea was developed using dispersive solid-phase extraction(dSPE) combined with high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).The samples were ultrasonically extracted with 0.2% formic acid,and then enriched and cleaned up with Layered Double Hydroxides(Mg-Al-LDHs) absorbent.The analytes were derived with fluorenylmethylchloroformate(FMOC-Cl) in sodium carbonate solution for 2 h.The separation of the target compounds were performed on a Phenomenex Kinetex C18 column(50 mm×2.1 mm,2.6 μm) using acetonitrile-5 mmol/L ammonium acetate(containing 0.2% formic acid) as mobile phase by gradient elution.The identification and quantification of the drugs were carried out by positive electrospray ionization(ESI+) in multiple reaction monitoring(MRM) mode,the quantification analysis was performed by the isotopically labelled internal standard method.The calibration curves showed good linearities in the range of 10-1 000 ng/mL with correlation coefficients(r2) more than 0.999.The limits of detection(LOD) and the limits of quantitation(LOQ) were 0.015 mg/kg and 0.05 mg/kg,respectively.The average recoveries of PMG and AMPA in different matrices at spiked levels of 0.05,0.1 and 1.0 mg/kg were between 866% and 95.7%,with relative standard deviations(RSDs) of 5.1%- 12.2%.The developed method is simple,rapid and sensitive,and is suitable for the rapid determination of trace PMG and AMPA residues in tea.关键词：dispersive solid-phase extraction(dSPE);high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS);tea;glyphosate;aminomethylphosphonic acid
- 2624
- |
- 704
- |
- 8
发布时间：2023-05-18 - 摘要：A rapid and reliable analytical method,based on large-volume injection and gas chromatography-mass spectrometry(PTV/GC-MS) was developed for the determination of 12 benzotriazoles and benzothiazoles in aquatic matrixes.1.0 L water samples were acidified to pH 3.0 using 3 mol/L HCl.The samples were then passed through an Oasis HLB cartridge.The analytes were eluted with 6 mL methanol-acetonitrile(1∶1).The solution was evaporated to dryness under a gentle stream of nitrogen,and redissolved in acetone,internal standard and silylation reagent.The sealed vials were incubated at 38 ℃ for 1 h before instrument analysis.The separation of target compounds was achieved in a DB-5MS fused-silica capillary column.The analytes were detected in selected ion monitoring(SIM) that operated in electron impact(EI) mode.The calibration curves of 12 target compounds were linear in the range of 0.1-100 ng/L with correlation coefficients not less than 0.992.Spiked recoveries of the target analytes were in the ranges of 46%-116% for tap water,and 43%-117% for river water,with RSDs of 1.0%-16% and 1.0%-15%,respectively.The method detection limits for tap water and river water were in the ranges of 0.050-1.3 ng/L and 0.057-1.8 ng/L,respectively.The method was simple and sensitive,and was successfully employed in the detection of corrosion inhibitors in river water collected from the city section of the Pearl River.关键词：corrosion inhibitor;solid phase extraction;silylation;gas chromatography-mass spectrometry(GC-MS);river water
- 2246
- |
- 529
- |
- 0
发布时间：2023-05-18 - 摘要：A method was developed for the determination of amantadine hydrochloride，rimantadine hydrochloride，dexamethasone，tilmicosin and olaquindox metabolite residues in poultry tissues by high performance liquid chromatography coupled with tandem mass spectrometry.The samples were extracted with acetonitrile after hydrolyzed with 2 mol/L sodium hydroxide and adjusted to pH value with hydrochloric acid.The extract was purified with a C18 solid phase extraction(SPE) cartridge，and eluted with methanol(containing 0.1% formic acid) and ammonia methanol(containing 0.1% ammonia).The target compounds were separated with a Phenomenex Kinetex C18(100 mm×4.6 mm，2.6 μm) by gradient elution using 0.1% formic acid containing 5 mmol/L ammonium acetate-methanol as mobile phase，and detected by liquid chromatography-tandem mass spectrometry in ESI under positive ion mode.The calibration curves were linear in the range of 2-100 μg/L for five drugs，with correlation coefficients of 0.996 2-0.999 8.The spiked recoveries of five drugs in poultry tissues at spiked levels of 5-50 μg/kg were in the range of 73.7%-92.3% with relative standard deviations(RSD,n=5) of 3.9%-16.6%.The detection limits(S/N≥3) for five drugs were in the range of 0.2-3.0 μg/kg and the quantitation limits(S/N≥10)were 0.7-10 μg/kg，respectively.The method is rapid，simple，economical and practical，and conforms to regulations requirement.Therefore，the method could meet the demand for routine analysis.关键词：amantadine hydrochloride;rimantadine hydrochloride;dexamethasone;tilmicosin;olaquindox-metabolite;solid-phase extraction(SPE);high performance liquid chromatography-tandem mass spectrometry
- 2126
- |
- 681
- |
- 6
发布时间：2023-05-18 - 摘要：A new label-free method for the detection of Ag+ was developed.The G-rich DNA sequences with many C loop residues can be folded into quadruplex structures with thioflavin T（ThT） as inducer,but this quadruplex structure can be destroyed by Ag+ ions,thus a novel label-free biosensor was designed based on this principle.A detection limit as low as 60 nmol/L and a wide dynamatic range of 0.1-10 μmol/L were achieved in this assay.The method was applied in the detection of silver ions in samples from Xiangjiang water and tap water with satisfactory results.In view of these advantages,the developed method was expected to provide an intrinsically robust,highly sensitive and specific sensing platform for the analysis of Ag+ in complex system.关键词：G-quadruplex;silver ion(Ag+);thioflavin T（ThT）;sensor
- 2949
- |
- 457
- |
- 5
发布时间：2023-05-18 - 摘要：An acetylene black/poly amino benzene sulfonic acid modified electrode(AB/PABSA/GCE) was prepared using electrical aggregation and coating.The modified electrode was characterized by electrochemical impedance spectroscopy(EIS).The electrochemical behavior of vanillin(Van) on AB/PABSA/GCE was investigated by cyclic voltammetry(CV).The results showed that there was an obvious oxidation peak of Van on the modified electrode in pH 7.0 PBS buffer solution and the oxidation peak current was linear with the scanning rate in the range of 40-300 mV/s,which indicated that the electrode process of Van was irreversible and controlled by absorption.The electron number(n),proton number(m),effective area(A),diffusion coefficients(D) and the absorbance of reactant were calculated to be 2,2,0.065 7 cm2,1.557×10-3 cm2/s,2.249×10-8 mol/cm2 in the electrode reaction process.The different concentrations of Van were determined by chronoamperometry,and a good linear relationship between oxidation peak current and Van concentration was observed in the range of 5-460 μmol/L.The detection limit(S/N=3) was 2.09×10-7 mol/L.The method was successfully applied in the determination of Van in chocolate sample with recoveries of 93.0%-114.2%.关键词：acetylene black;amino benzene sulfonic acid;vanillin;electrical aggregation;chronoamperometry
- 2091
- |
- 444
- |
- 0
发布时间：2023-05-18 - 摘要：The purified performance of three different types of solid-phase extraction columns,including HLB column,C18 column and PEP column,was investigated in this study.And based on that,a solid phase extraction/high performance liquid chromatography was developed for the simultaneous determination of 8 antibiotic residues in dairy.The result indicated that the HLB column showed an excellent elution and purification effect,and it was applicable for the dairy matrix purification.Under the optimal conditions,the calibration curves of eight antibiotics were linear in the concentration range of 5.0-100.0 μg/L with correlation coefficients(r2) more than 0.999.The limits of detection(LOD) and limits of quantitation(LOQ) of 8 antibiotics were in the range of 0.09-0.84 μg/kg and 0.32-2.23 μg/kg, respectively.The recoveries of target compounds at spiked concentration levels of 5,10 and 30 μg/kg ranged from 73.2% to 93.3%,with relative standard deviations(n=5) of 1.4%-3.6%.The developed method was suitable for the analysis of antibiotic residues in the complex dairy matrix.关键词：high performance liquid chromatography(HPLC);solid phase extraction(SPE);dairy;antibiotics residues;purification
- 2041
- |
- 475
- |
- 7
发布时间：2023-05-18 - 摘要：Volatile phenols,the toxic organic compounds,are listed as the environmental priority controlled pollutants.The content level of them has become one of the important indicators for environmental pollution evaluation.This review briefly states the chemical characteristics and geoenvironmental sources of volatile phenols,summarises the relevant domestic and international standard methods,concludes the research progress on application of sample pretreatment technologies(e.g.solvent extraction,distillation,solid phase extraction,solid phase microextraction,purge and trap) and analytical methods(e.g.4-amino antipyrine spectrophotometry,ultraviolet and fluorescence spectrophotometry,bromine method,gas chromatography,liquid chromatography,phenol biosensor method) in the analysis of volatile phenols in geoenvironmental samples.The future development trend about the analysis of volatile phenols is also prospected. The review provides a reference for the further study on analytical methods of volatile phenols and their environmental applications.关键词：volatile phenols;geoenvironmental samples;pretreatment;analytical methods;review
- 2058
- |
- 645
- |
- 6
发布时间：2023-05-18
- Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
- Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
- Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
0