最新刊期
卷 33 ， 期 10 ， 2014
- 摘要：Immunoaffinity column（IAC） for T-2 toxin was prepared with anti-T-2 toxin monoclonal antibody 2G7 and CNBr activated Sepharose 4B.The experimental conditions for T-2 immunoaffinity column were optimized and the columns were applied in cereals and feed pretreatment.An IAC/LC-MS/MS method was established for the determination of T-2 toxin in cereals and feed.The calibration curve for T-2 toxin standard solution was linear in the range of 0.5-500.0 ng/g with correlation coefficient of 0.999 7.The limit of detection（LOD） and limit of quantitation（LOQ） was 0.05 ng/g and 0.17 ng/g，respectively.The average recoveries of T-2 toxin in rice，maize and feed samples at three spiked levels of 10，50，100 ng/g ranged from 92.9% to 109.7%，with relative standard deviations（RSDs） of 2.1%-8.3%.The method was applied in the detection of T-2 toxin in 36 real samples with RSDs less than 10.0%.关键词：T-2 toxin;immunoaffinity column;LC-MS/MS;quantitative analysis;cereals and feed
- 2327
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- 1108
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- 2
发布时间： - 摘要：A rapid method for the simultaneous determination of 18 weakly acidic pesticide residues including famoxadone in fruits and vegetables was developed using liquid chromatography-tandem mass spectrometry（LC-MS/MS）.The samples were extracted with acetonitrile containing 1% acetic acid，and salted out with anhydrous magnesium sulphate and anhydrous sodium acetate.The extracts were cleaned up by dispersive solid phase extraction using C18 as sorbents.The ionization efficiency of famoxadone during atmospheric pressure ionization was studied.The result showed that ammonium acetate provided can significantly improve the ionization efficiency of famoxadone under negative electrospray ionization mode.Chromatographic analysis was carried out on a CSH C18 column using 5 mmol/L ammonium acetate-acetonitrile as mobile phase by gradient elution.Pesticides were analyzed by negative electrospray ionization tandem mass spectrometry under multiple reaction monitoring mode.Quantification was achieved using matrix-matched standard calibrations as the external standard.Recoveries of 18 residues in 8 matrixes（broccoli，shallot，carrot，green bean，shiitake mushroom，apple，kiwifruit and orange） at fortification levels of 10,20,100 μg/kg ranged from 71.4% to 119.7% with relative standard deviations of 1.2%-10.0%.The limits of quantitation（S/N≥10） were in the range of 0.1-2.0 μg/kg.The method was simple，sensitive and environmental，and was suitable for monitoring 18 acidic pesticides residues in fruits and vegetables.关键词：QuEChERS;liquid chromatography-tandem mass spectrometry（LC-MS/MS）;famoxadone;acidic pesticide;fruit;vegetable
- 2227
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- 1067
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- 21
发布时间： - 摘要：A method was developed for the determination 16 perfluorinated compounds(PFCs) in egg by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) combined with dispersive solid phase extraction.The samples were extracted with acetonitrile in an ultrasonic bath at 40 ℃.The extract was concentrated with N2, purified with graphitized carbon black（GCB）,and analysed by HPLC-MS/MS.The calibration curves of 16 PFCs were linear in the concentration range of 0.50 -10.0 μg/L with correlation coefficients(r) of 0.996 2-0.999 9.The limits of detection for 16 PFCs were between 0.001 μg/kg and 0.093 μg/kg,and the limits of quantitation were between 0.003 μg/kg and 0.310 μg/kg.The mean recoveries of 16 PFCs at spiked levels of 0.5,1.0,2.0 μg/kg were in the range of 79%-125% with relative standard deviations(RSDs,n=6) of 0.26%-14%.The method was suitable for the identification and quantification of 16 perfluorinated compounds in egg due to its advantages of simplicity,accuracy and high sensitivity.关键词：perfluorinated;egg;high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS);dispersive solid phase extraction;GCB
- 2298
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- 3
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- 17
发布时间： - 摘要：The ACE inhibitory activities of four food-derived tripeptides，including IRP，IKP，GRP and IRA，were verified by some experiments.However，their interaction modes with ACE and molecular mechanism remain unclear.This research focused on resolving the above problems using flexible molecular docking method.The molecular docking results demonstrated that four peptides had similar action modes with ACE.Hydrogen bond，hydrophilic，hydrophobic and electrostatic interactions were together responsible for the conformational stability of the complexes formed by peptides and ACE，in which hydrogen bond was the most important interaction.Amino acid residues Lys511，His513，Tyr520，Tyr523 in ACE molecule were the most important binding sites combined with active peptides.N-terminal amino groups and C-terminal carboxyl of ACE inhibitory peptides were the key groups for the activities of tripeptides，especially the N-terminal amino groups.The above information will be helpful for the development of ACE inhibitory peptides with high activities.关键词：angiotensin I-converting enzyme;inhibitory tripeptides;molecular docking;molecular mechanism
- 2072
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- 1075
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- 5
发布时间： - 摘要：A normal high performance liquid chromatography(HPLC) with chiral stationary phase for detection of enantiomer and diastereomers in solifenacin succinate bulk drug was developed.The separation efficiencies of Daicel CHIRALPAK? AD-H column，Daicel CHIRALCEL? OJ-H column，Research ＆ Cretivity SCDP52546 column and Research ＆ Cretivity RC-OD52546 column for 4 optical isomers were investigated.Various UV detection wavelengths，column temperatures and mobile phases types were also investigated.The best chromatographic conditions were as follows:column:Daicel CHIRALPAK? AD-H column，mobile phase:n-haxane-ethanol-diethylamide（900∶100∶1），UV detection wavelength:220 nm，flow rate:1 mL/min，column temperature:30 ℃.The method had a good specificity（resolution（R）≥2.0） and a good precision（RSD≤2.0%，n=6）.The calibration curves showed good linearities over the ranges of 0.240 5-10.822 5 μg/mL for enantisomer，0.186 8-8.406 0 μg/mL for diastereoisomerⅠand 0.196 1-8.824 5 μg/mL for diastereoisomerⅡ with correction coefficients more than 0.999.The limits of detection（LODs） were in the range of 0.062 25-0.080 13 μg/mL and the limits of quantitation(LOQs) were 0.186 8-0.240 5 μg/mL.The average spiked recoveries were in the range of 94.4%-95.7% with relative standard deviations of 2.5%-3.1%.The method was simple，sensitive and valid，and could satisfy the requirements for detection of enatiomer and diastereoisomers in solifenacin succinate drug substance.关键词：solifenacin succinate;high performance liquid chromatography(HPLC);enantiomer;diastereoisomer
- 2672
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- 1271
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- 7
发布时间： - 摘要：A method for the simultaneous separation of seven kinds of drugs including tetracyclines（TCs） and penicillins（PENs） by micelle capillary electrophoresis（MEKC） was developed.Effects of some important factors such as composition，pH value and concentration of running buffer，surfactant concentration，separation voltage and separation temperature were investigated，and the factors were optimized by the L16（45） orthogonal test.The optimal conditions were as follows：the running buffer：40 mmol/L potassium dihydrogen phosphate-20 mmol/L borax solution（pH 7.9），SDS concentration：65 mmol/L,separation voltage：28 kV，separation temperature：28 ℃，UV detection wavelength：350 nm and 200 nm．Seven kinds of drugs were baseline separated within 25 min.The calibration curves showed good linearities in the range of 1.56-50 mg/L，with correlation coefficients（r2） of 0.997 9-0.999 9．RSDs（n=6） were in the range of 4.1%-7.3% for peak area and 0.33%-0.67% for migration time.The average recoveries of seven kinds drugs at three spiked concentration levels of 2.0，5.0，10.0 mg/kg ranged from 83.6% to 93.3%，with RSDs（n=6） of 4.7%-7.6%．The method was rapid，simple，accurate and high sensitive,and was successfully applied in the determination of seven kinds of drugs in the ponds and lakes of Hefei and its surrounding areas.关键词：micelle capillary electrophoresis(MEKC);simultaneous separation;tetracyclines(TCs);penicillins(PENs)
- 2007
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- 963
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- 1
发布时间： - 摘要：Head space solid-phase micro-extraction（HS-SPME） coupled with gas chromatography-mass spectrometric（GC-MS） method was developed for the analysis of Wuxi baikhovi tea.Effects of SPME fiber type，extraction temperature，preheating time，adsorption time，particle size of tea and mass of tea on kinds and amounts of aroma compounds in baikhovi tea were studied.The optimum conditions were as follows:2.0 g Wuxi baikhovi tea was used in 70 mesh，SPME fiber:carboxen（CAR）/divinylbenzene（DVB）/polydimethylsiloxane（PDMS） fiber，extraction temperature:80 ℃，preheating time:5 min，adsorption time:40 min.After the extraction，the fiber was inserted into the injector port of GC-MS system and the extract was desorbed at 250 ℃ for 3 min.Total of 53 volatile compounds were identified in Wuxi baikhovi tea.Among them,cis-3-hexen-1-ol and its esters，linalool and its oxides，phenethyl alcohol，geraniol，nerolidol，farnesene，cis-jasmone，ionone，indole and dimethyl sulfide are more important to the total green，floral and fruity aroma of Wuxi baikhovi tea.关键词：HS-SPME;GC-MS;Wuxi baikhovi tea;volatile components
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- 10
发布时间： - 摘要：In this paper，poly（L-glutamic acid ethyl ester） was synthesized.After poly（L-glutamic acid ethyl ester） was dissolved in chloroform，a chiral stationary phase based on the poly（L-glutamic acid ethyl ester） coated onto silica gels was successfully prepared.The chiral stationary phase was used for the separations of 4-racemates(furoin,omeprazole,naproxen,tetramizole-hydrochloride) and 9-isomers(o,m,p-iodoaniline,o,m,p-chloroaniline,o,m,p-bromoaniline,o,m,p-nitroaniline,o,m,p-toluidine,o,m,p-chlorophenol,o,m,p-dinitrobenzene,o,m,p-benzenediol,o,m,p-aminophenol) with HPLC using different ratios of hexane-isopropanol and hexane-ethanol as mobile phases.The experimental results showed that poly（L-glutamic acid ethyl ester） possesses a good ability for separating enantiomers and isomers.关键词：poly L-glutamic acid ethyl ester;chiral stationary phase;positional isomers;high performance liquid chromatography(HPLC)
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- 1070
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- 3
发布时间： - 摘要：A rapid method was established for determination of five fluoroquinolones antibiotic residues in milk.Sample solution was injected after protein precipitating with 0.2% trichloroacetic acid-acetonitrile(90∶10) .Turboflow column was used as SPE column to enrich sample through large volume injection and cleanup with left pump of dual gradient LC.Strong solvent stored in 100 micro-liter loop was used to transfer the analyte which was diluted with right pump of DGLC in online mode.Then peak shape and resolution were improved by peak refocusing effect.The limits of detection(LODs) were between 0.01 μg/L and 0.13 μg/L by fluorescence detection，which were far below the maximum residue levels for milk set by the EU regulations. The linearity of this method was good in the certain concentration range.The mean recoveries were in the range of 75.9%- 95.7%.The results indicated that this method is simple，rapid and sensitive，and is suitable for the determination of five fluoroquinolones antibiotic residues in milk.关键词：turbulent flow chromatography;online SPE;liquid chromatography;fluoroquinolones;milk;online dilution;veterinary residue
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- 1001
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- 5
发布时间： - 摘要：A method of ion-exchange chromatography with direct conductivity detection to determine three homologous quaternary ammonium cations(tetramethyl-ammonium cation,tetraethyl-ammonium cation and tetrapropyl-ammonium cation) was developed Three homologous quaternary ammonium cations were separated on a sulfonic acid base cation-exchange column using ethylenediamine-citric acid-acetonitrile as eluent.Effects of eluent types,eluent concentrations and column temperatures on retention and separation of three cations were discussed.The separations of the cations were improved by changing concentration of ethylenediamine and acetonitrile in eluents.With the increase of acetonitrile concentration,the retention time of tetrapropyl ammonium cation was obviously reduced,and the chromatogrophic peaks of the cations were improved.The retentions of homologous quaternary ammonium cations accord with carbon number rule.The suitable eluents for analysis of tetramethyl-ammonium cation and tetraethyl-ammonium,tetrapropyl ammonium cations were 0.02 mmol/L ethylenediamine-0.12 mmol/L citric acid(pH 4.0) and 0.2 mmol/L ethylenediamine-0.4 mmol/L citric acid-1% acetonitrile(pH 4.0),respectively.The flow rate and column temperature were 1.0 mL/min and 40 ℃,respectively.The detection limits(S/N=3) for three cations were 0.015,0.22,1.88 mg/L,respectively.Relative standard deviations(n=5) for peak areas were less than 2.3%.The method was successfully applied in the determination of quaternary ammonium ionic liquids synthesized in chemical laboratory and surfacetant tetramethyl-ammonium hydroxide,with recoveries of 99%-104%.The method is simple,accurate,reliable and practical.关键词：quaternary ammonium cations;ion-exchange chromatography;conductivity detection;ionic liquids
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- 980
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- 7
发布时间： - 摘要：A high performance liquid chromatographic（HPLC）method was established for the simultaneous separation and determination of α-bromocinnamaldehyde（α-BCA），thiabendazole（TBI），triclosan（TCC） and triclocarban（TCS） in textiles.The samples were dissolved with ethyl acetate and extracted by ultrasonic extraction.The extract was concentrated and filtrated，then the separation of analytes was preformed on a symmetry C18（4.6 mm i.d.×250 mm，5 μm） column，with methanol-tetrahydrofuran-20 mmol/L NH4Ac as mobile phase（60∶20∶20） using isocratic elution at a flow rate of 0.8 mL/min.The photodiode array detector was set at 230，260，300 nm.The results indicated that four substances were completely separated in 15 min.The calibration curves showed good linearities in the ranges of 0.2-100 mg/L for α-BCA and TBI，0.1-100 mg/L for TCC，and 0.3-100 mg/L for TCS，with correlation coefficients of 0.998 8-0.999 9.The recoveries of four substances were in the range of 80.5%-104.9%，with RSDs below 7.2%.The quantitation limits for α-BCA，TBI，TCC，TCS（LOQ，S/N≥10） in three kinds of textiles were in the ranges of 0.2-0.5,0.2-0.4,0.1-0.2,0.3-0.6 mg/kg，respectively.This method was simple，rapid and sensitive，and was applied in the determination of organic antibacterial and antifungal agents in textiles with satisfactory results.关键词：textiles;α-bromocinnamaldehyde;thiabendazole;triclosan;triclocarban;high performance liquid chromatography（HPLC）
- 2045
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- 991
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- 1
发布时间： - 摘要：The effect of sensitivity enhancement of time-of-flight mass spectrometer(TOF MS) was studied with home made ion enrichment pulse power.Ion enrichment pulse power includes pulse signal generating module,amplification and isolation module,switching driving module,high voltage circuit module and load impedance matching module,outputing one medium voltage pulse signal and two-way symmetric high voltage pulse signals.In this work,TOF MS with electrospray ionization(ESI) was combined with laser desorption ionization(LDI) to debug ion enrichment pulse power.As a conclusion,the detection sensitivity of the instrument could be increased by 6-24 times for different samples when applying ion enrichment pulse power.关键词：sensitivity;ion enrichment pulse power;time-of-flight mass spectrometer(TOF MS)
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- 1066
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发布时间： - 摘要：In this work,NMR spectral characteristics of doxepin compounds were investigated with various experimental conditions,taking 11-（3-dimethylaminopropylidene）-6,11-dihydro-dibenzo ［b,e］oxepine-2- carbaldehyde（DP） as the testing sample.The conformation exchange of DP was confirmed.It was proved that the NMR spectral characteristics would depend on the experimental conditions,and the temperature would greatly affect the characteristics of the signals.Under the room temperature,the phenomena of signal broadening and missing were observed.As a result,the NMR signal assignment could not be accomplished.These phenomena could be improved with increase of temperature.The abnormal phenomena were effectively inhibited at 80 ℃.Finally,the complete assignment was presented and configuration was confirmed.关键词：doxepin compounds;analysis for spectra;NMR;conformation exchange
- 1989
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- 1052
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- 0
发布时间： - 摘要：To study the residues of seven kinds of indicator polychlorinated biphenyls in aquatic products，a method was established for the determination of trace polychlorinated biphenyls in aquatic products by double depuration coupled with gas chromatography(GC).With the mixture of acetone-n-hexane(1∶4) as extract，the samples were extracted,purified by adding concentrated sulfuric acid followed by clean up with silica gel in dispersive solid phase extraction protocol，and then analyzed by gas chromatography.Under the optimized chromatographic conditions，the analysis was carried out on an HP-5 quartz capillary column(30 m×0.32 mm×0.25 μm) at a flow rate of 0.80 mL/min，and the sample volume was 1.00 μL.Monitored with an electron-capture detector，all the target analytes were separated by “temperature-programming ”.Good linearities were obtained in the range of 10-500 μg/kg for seven indicators of polychlorinated biphenyls(PCBs) with correlation coefficients greater than 0.99.For different matrices，the limits of detection(S/N=3) and limits of quantitation(S/N=10) were in the ranges of 0.72-4.1 μg/kg and 2.4-13.7 μg/kg，respectively.At three spiked levels of 10，20,100 μg/kg，the average recoveries of seven indicators of polychlorinated biphenyls(PCBS) from butterfish，shrimp and mussel samples ranged from 76.5% to 104.8% with RSDs of 1.6%-10.4%.The method was simple，rapid and accurate，and could be used for the routine analysis of indicator polychlorinated biphenyls(PCBs) in aquatic products.关键词：double depuration;gas chromatography(GC);polychlorinated biphenyls(PCBs);residues;aquatic products
- 2094
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- 1069
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- 5
发布时间： - 摘要：A high performance liquid chromatography tandem mass spectrometric（HPLC-MS/MS） method was developed for the determination of amantadine residues in pepper and pepper products.After extracted with 1% trichloroacetic acid solution-methanol（1∶1） and purified by an Oasis-MCX SPE cartridge，the samples were sepatated on an Atlantis T3 C18 column（3.0 μm，2.1 mm× 150 mm）by gradient elution.The electrospray was operated in positive mode and the samples were monitored under the multiple reaction monitoring（MRM） mode.The calibration curves showed good linearity in the range of 1.0-50 μg/kg.The quantitation limit was 1.0 μg/kg for fresh pepper，and 5.0 μg/kg for dried pepper and chili products.The recoveries of amantadine in fresh pepper at spiked levels of 1.0，2.0，5.0 μg/kg were 72.2%-98.2%，with relative standard deviations（RSDs，n=6） of 1.2%-4.6%，and recoveries in dry pepper and chili products at spiked levels at 5.0，10.0，25.0 μg/kg were 64.2%-81.6%，with RSDs（n=6） of 1.3%-6.3%.With advantages of simple operation，high sensitivity，good reproducibility，the method could be used for the detection of amantadine residues in the pepper and pepper products.关键词：high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）;amantadine;pepper;pepper products
- 2521
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- 1054
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- 0
发布时间： - 摘要：The rapid quantification method of human serum glucose was established by using the Fourier transform infrared spectroscopy（FTIR） and attenuated total reflection（ATR）.The subtracted spectrum between glucose solution and water was calculated which was 1 200-900 cm-1 and named fingerprint region.Both partial least squares（PLS） models of whole spectrum（4 000-600 cm-1） and fingerprint spectrum were built.The predicted result of fingerprint region was better than that of the whole spectral.Three PLS models for all samples，low concentration samples and high concentration samples were built.Based on this,compound decision of three models was carried on.Finally，the independent test set was performed for model verification.The results showed that the prediction bias of the combination model for human serum glucose was obviously better than that of the whole spectral or the fingerprint region，and for the samples whose value were nearby threshold，the difference of prediction errors for low concentration model and high concentration model was small.RMSEP and RP were 0.732 mmol/L and 0.948，respectively，by the concentration section model.关键词：Fourier transform infrared spectroscopy（FTIR）;attenuated total reflection（ATR）;serum glucose;partial least squares（PLS）;combination model
- 2137
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- 1026
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- 0
发布时间： - 摘要：A simple and low-cost composite film was prepared,based on which a sensitive detection method of lead ions(Pb2+) in aqueous solutions with naked eye was developed.Polyamide-6(PA-6) thin layer chromatographic membrane was selected to adsorb bovine serum albumin(BSA) modified gold nanoparticle(AuNPs),and a stable BSA-AuNPs/PA-6 nanocomposite film with a uniform red color was prepared.The adsorption time of BSA-AuNPs to PA-6 membrane,concentrations of Na2S2O3 and 2-ME as well as reaction temperature and time in the 2-ME-Na2S2O3-AuNPs/Pb2+ leaching system were investigated,which demonstrated obvious effects on the detection of Pb2+.Under the optimal conditions(BSA-AuNPs and PA-6 incubate together for 30 min,0.05 mol/L Na2S2O3,0.25 mol/L 2-ME,nanocomposite film and leaching agents incubate together at 80 ℃ for 20 min),the BSA-AuNPs/PA-6 nanocomposite film allowed for the sensitive detection of Pb2+ with naked eye with a sensitivity as low as 2.0×10-8 mol/L.The method showed a high selectivity,and was applied in the naked eye detection of Pb2+ in tap water with a detection limit of 5.0×10-8 mol/L.关键词：gold nanoparticle;nanocomposite film;lead ions;naked eye detection
- 2150
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- 1105
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- 1
发布时间： - 摘要：Chinese materia medica Tong-Guang-San（Marsdenia tenacissima） is used for treatment of cancer.Developing a bioactive fraction from this plant with optimized components as antitumor agent or chemosensitizer is of great importance.This paper reports results of methodological research for quality control of extract of total glycosides（ETG），a chemosensitive fraction screened out.In our study，ETG at the concentration of 10 μg/mL did not affect the growth of human leukemia cell line K562，human cervical carcinoma cell line HeLa，human hepatoma cell line HepG2 and human oral epidermoid carcinoma cell line KB-3-1（Cell viability＞98%），but when 10 μg/mL ETG was used in combination with paclitaxel，the cytoxicity of paclitaxel against above tumor cells was significantly increased.By employing a Phenomenex Luna C18 column as stationary phase，methanol-0.1% HAc as mobile phase for gradient elution，and 6 ester derivatives of tenacigenin B（1-6） as reference markers，an HPLC method was used for the qualitative analysis of ETG，with well separation of 25 components and identification of the 6 structurally characteristic markers.The summed peak area for 1-6 accounted for 43.39% of the total peak area.The quantitative analysis of reference 1 was also completed，with relative standard deviations（RSD） for peak area，repeatability of measured concentration and stability of sample within 0.16%-0.55%，linear range of 0.86-6.45 μg，and recoveries of 100.4%±1.2%.The method was applied in the determination of total glycosides of Marsdenia tenacissima with reference 1 amount of 0.74%.关键词：Marsdenia tenacissima;total glycosides;tenacigenin B ester derivative;HPLC;chemosensitivity
- 2035
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- 1016
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- 1
发布时间： - 摘要：A liquid chromatography coupled with electrospray ionization tandem mass spectrometric（LC-MS/MS） method was developed for the determination of phenazine-1-carboxylic acid in ginseng.The ginseng samples were soaked with water，extracted with acetonitrile and cleaned up by dispersive solid phase extraction，separated by chromatography，then detected by MS/MS under multiple reaction monitoring（MRM） mode via positive electrospray ionization.The ion-pair of 225.1/179.1 was used for quantitative analysis，and the ion-pair of 225.1/125.1 was selected for qualitative analysis.The external standard method was used for the quantification analysis.The limit of quantitation（LOQ） of phenazine-1-carboxylic acid was 0.01 mg/kg.The standard calibration curve was linear in the range of 0.5-10 μg/L，with correlation coefficient more than 0.99.The recoveries of phenazine-1-carboxylic acid in ginseng were in the range of 82%-100% at three spiked levels of 0.01，0.02 and 0.10 mg/kg，with relative standard deviations（RSDs） not more than 6.8%.The result showed that the method was simple，rapid and highly sensitive,and could be applied in the determination of phenazine-1-carboxylic acid in ginseng.关键词：liquid chromatography-tandem mass spectrometry（LC-MS/MS）;phenazine-1-carboxylic acid;ginseng
- 2079
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- 907
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- 1
发布时间： -
Flourescence Enhancement of CT2R by Silver Nanoparticles and Its Application in Determination of BSA
摘要：Under acidic conditions，the flourescence of CT2R could be enhanced with silver nanoparticles because of its surface enhancement effect.This enhancement effect would be decreased in the presence of BSA.The decreased fluorescence intensity is proportional to the concentration of BSA.According to this principle，a simple and new method for BSA determination was developed.The influences of some experimental factors，including pH value，concentration of CT2R，concentration of silver nanoparticles，addition order of reagents on the determination of BSA were investigated.Under the optimal conditions，the linear range for BSA detection is 0.02-1.00 mg/L，with a detection limit of 0.002 6 mg/L.The method was used in the detection of BSA in synthetic samples with satisfactory results.关键词：chromotropic acid 2R;silver nanoparticles;fluorescence spectroscopy;surface enhancement effect- 2095
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- 932
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