最新刊期
卷 32 ， 期 10 ， 2013
- 摘要：An HPLC-ESI-iFunnel-MS/MS method was established for the simultaneous determination of 20 forbidden veterinary drugs in swine urine.The urine matrix was directly purified by HLB solid phase extraction cartridges and blown dry with nitrogen after eluted with methanol，then redissolved with acetonitrile-water（1∶9,by volume） to 1 mL.The target compound was separated on an Eclipse Plus C18 column and detected by MS/MS under electrospray ionization in positive ion mode.The iFunnel technology was used to focus ions and multiple reaction monitoring（MRM） was used to monitor ions.Standard curve method was used for quantitative analysis.Good linearities of 20 forbidden veterinary drugs were observed in the range of 0.1-50 μg/L with correlation coefficients higher than 0.99.The average recoveries at three spiked concentration levels ranged from 81.5%to 104.8%，with relative standard deviations（RSDs） of 3.0%-16.0%.The limits of detection（LOD） were in the range of 0.05-0.2 μg/L and the limits of quantitation（LOQ） were 0.1-0.5 μg/L.The established method was used for the determination of 20 forbidden veterinary drugs in real urine samples collected from a farm and laboratory，and none of the 20 forbidden veterinary drugs was detected.With the advantages of rapidness，sensitivity and high throughput，this method could meet the requirements for residue limits detection at home and abroad.关键词：iFunnel;urine;veterinary drug;solid phase extraction（SPE）
- 2056
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- 1078
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- 1
发布时间： - 摘要：A sample cleanup procedure for the simultaneous determination of PCDD/Fs,PBDD/Fs,PCBs and PBDEs in soil was developed.Firstly,four series of halogenated organic contaminants were separated into chlorinated(PCBs and PCDD/Fs) and brominated(PBDEs and PBDD/Fs) groups,with multi-layer gel column containing silver nitrate silica.Florisil column was subsequently used to separate PCBs from PCDD/Fs and PBDEs from PBDD/Fs,respectively.The results indicated that the four series contaminants could be completely separated.Recoveries and relative standard deviations(RSDs) of standard-spiked samples were as follows:PCDD/Fs:61%-83%,1.4%-14.2%;PBDD/Fs:71%-110%,2.8%-14.9%;PCBs:68%-92%,3.6%-15.2%;PBDEs:81%-87%,10.9%-17.4%.This method was applied in the simultaneous detection of four series of compounds in soil samples,and the results were no obvious difference with that of the reported method(the deviation of two methods was not more than 12.5% )，which implys that the developed method has good accuracy and reliability.关键词：PCDD/Fs;PBDD/Fs;PCBs;PBDEs;soil
- 2642
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- 1169
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- 1
发布时间： - 摘要：Hydrophilic graphene nanosheets were rapidly synthesized by reacting graphene oxide slice layer with poly（sodium 4-styrene sulfonate)（PSS) and simultaneously reduced with hydrazine hydrate under hydrothermal conditions.High resolving transmission electron microscopy（HRTEM) analysis indicated that the PSS modified transparent graphene nanosheets are entangled with each other and resemble rippled silk.The direct electrochemistry of hemoglobin（Hb) immobilized on the HAp and hydrophilic graphene（HG) composite film was studied and an amperometric biosensor of hydrogen peroxide was developed based on the direct electron transfer of Hb.Hb entrapped in HAp-HG film displayed a pair of well-defined redox peaks at about -0.295 V（vs-SCE) in 0.1 mol/L pH 7.0 phosphate buffer solution，which corresponded to the heme FeⅢ/FeⅡ redox couples.The resulting electrode showed an excellent electrocatalytic performance to the reduction of H2O2 with a fast amperometric response of about 5 s and could be used as a H2O2 biosenor with a lower detection limit of 0.58 μmol/L.关键词：hemoglobin;graphene;hydroxyapatite;hydrogen peroxide
- 1839
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- 1024
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- 1
发布时间： - 摘要：A new method for the purification of shrimp allergenic protein Pen a 1 was developed.The epitope peptide（aa 247 to 284） of the Pen a 1 was synthesized by standard Fmoc and conjugated to KLH and BSA to get the artificial immune antigen（peptide-KLH） and the coating antigen（peptide-BSA），respectively.New Zealand white rabbits were immunized with immunogen peptide-KLH to produce antisera and the antisera were purified by bitterness-ammonium sulfat and HiTrap rProtein A FF.The pure antiserum was coupled to CNBr-Activated Sepharose 4B.The titer of purified antibody was 2.05×106 by indirect ELISA and the IC50 was 0.21 mg/L by indirect competitive ELISA method.There was no cross-reactivity between the antibody and the proteins from shrimp except for Pen a 1 by indirect competitive ELISA method.The coupling efficiency of the antibody with CNBr-Activated Sepharose 4B was 90.76% and the column capacity for 1 mL immunosorbents was 2.84 mg Pen a 1.The recoveries of the immunoaffinity column were in the range of 89.6%-93.6%.The service life of the immunoaffinity column was 4 cycles.The SDS-PAGE and Western Blot results displayed that the purified Pen a 1 had high immunogenicity.关键词：Pen a 1;polyepitope antibody;immunoaffinity column;ELISA
- 1883
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- 984
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- 2
发布时间： - 摘要：An accelerated solvent extraction（ASE）/gas chromatography-tandem mass spectrometric（ASE/GC-MS/MS） method combined with solid phase extraction（SPE） was developed for the simultaneous determination of 71 pesticides residues in marine sediments．The best extraction temperature and static time were 100 ℃ and 5 min in 1 cycle，respectively.Under the conditions，the sample was extracted with a mixture of hexane-acetone（1∶1）,cleaned up on a Carbon SPE cartridge and determined by MS/MS under multiple reaction monitoring mode（MRM）.The calibration curves showed good linearities（r2＞0.99） in the range of 1.0 -500 μg?L-1 for all pesticides，and the limits of detection（LOD,3σ） ranged from 0.29 μg?kg-1 to 108.43 μg?kg-1.The recoveries of the pesticides at a spiked level of 100 μg?kg-1 were in the range of 51.3%-104.6%with RSDs（n=6） of 0.1%-13.5%.关键词：ASE;GC-MS/MS;marine sediments;pesticides
- 1881
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- 1013
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- 7
发布时间： - 摘要：Well-dispersed citrate-capped silver nanoparticles were synthesized in aqueous solution by reducing silver(Ⅰ) nitrate with sodium borohydride.As a nucleophilic agent，iodide ion could be easily adsorbed on the surface of silver nanoparticles through donating its electron pair on the unoccupied orbital of silver nanoparticles.Due to the low solubility constant(Ksp=8.52×10-17) of AgI，the addition of silver(Ⅰ) ion could induce the aggregation and color change of silver nanoparticles by forming AgI on the surface of iodide ion adsorbed silver nanoparticles.Based on this phenomenon，a highly sensitive colorimetric detection of silver(Ⅰ) ion was developed.Silver(Ⅰ) ion could be detected at 2.0 nmol/L by naked-eye.The linear range was 1.5-1 000.0 nmol/L，and the detection limit was 0.8 nmol/L when silver(Ⅰ) ion was detected by absorption spectrometric method.The common metal ions could not induce color change and absorption variation of iodide ion adsorbed silver nanoparticles even at the concentration of 2.0 μmol/L，indicating acceptable selectivity of the method to silver(Ⅰ) ion.High resolution transmission electron microscopy，dynamic light scattering and reason light scattering were used to investigate the detection mechanism of the system.All these characterization techniques confirmed the aggregation of silver nanoparticles when iodide ion and silver(Ⅰ) ion were added due to the loss of the negative charges of silver nanoparticles after the formation of neutral AgI.The developed method was used to detect silver(Ⅰ) ion in tap water and lake water by the standard addition methods with satisfactory results.关键词：silver nanoparticles;silver(Ⅰ) ion;colorimetry;absorption spectrum
- 2140
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- 1038
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- 1
发布时间： - 摘要：A novel electrochemical sensor for pyrazosulfuron-ethyl（PSE）detection based on molecularly imprinted polymer membrane was prepared by electropolymerization of o-aminophenol（oAP）at gold electrode in the presence of template molecule.The modification procedure was characterized via cyclic voltammetry and electrochemical impedance spectroscopy.And its recognition performance was evaluated by differential pulse voltammmograms（DPV）.Under the optimized conditions，the response current of K3Fe（CN）6 probe on the proposed sensor decreased linearly with PSE concentration in the range of 2-150 μmol/L with a detection limit of 0.65 μmol/L（r2=0.995）.With high selectivity and stability，the imprinted sensor was applied in the determination of PSE in environmental water samples with recoveries of 97.6%-104.4%.关键词：pyrazosulfuron-ethyl;molecular imprinting;electrochemical sensor;electropolymerization film
- 3358
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发布时间： - 摘要：Interaction of clenbuterol hydrochloride(CL) with human immunoglobulin G(HIgG) was studied using fluorescence quenching spectroscopy，three-dimensional fluorescence spectroscopy，UV-vis absorption spectroscopy and circular dichroism(CD) spectroscopy under simulative physiological conditions for the first time.The binding parameters and the thermodynamic parameters for the reaction of CL with HIgG at different temperatures were calculated according to Scatchard equation and Van-t Hoff equation，respectively.Experimental results of the fluorescence quenching spectra showed that the fluorescence intensity of HIgG was quenched by the gradual addition of CL.The binding constants of CL with HIgG at 298，304,310 K were calculated to be 2.95×104，2.35×104,1.82×104 L/mol，respectively，meaning that the quenching mechanism was a static quenching.Meanwhile，the corresponding numbers of binding sites(n) were calculated to be 0.84，0.87 and 0.94，respectively.The thermodynamic parameters of the reaction，namely standard enthalpy ΔH0 and entropy ΔS0，were calculated to be-30.47 kJ?mol-1 and-16.58 J?K?mol-1，respectively，suggesting that there were several forces in the binding of CL with HIgG,and the hydrogen bond and Vander Waals force were the predominant intermolecular forces in stabilizing the CL-HIgG complex.Experimental results obtained from the three-dimensional fluorescence spectroscopy and UV-vis absorption spectroscopy confirmed that the secondary structure of HIgG was altered in the presence of CL in aqueous solution.Furthermore，a decrease of the β-structure compositions of HIgG was observed by CD spectroscopy when the molar concentration ratio of HIgG to CL was 1∶4.关键词：clenbuterol hydrochloride;human immunoglobulin G;spectroscopy;interaction
- 2340
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- 1105
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- 2
发布时间： - 摘要：An ultra performance liquid chromatography-mass spectrometric（UPLC-MS）method was developed for the simultaneous determination of purine nucleotides（e.g.ATP，ADP，AMP，NAD+ and NADP+） in mammalian cell.The HPLC separation was carried out on a KinetexTM HILIC column（50 mm×4.6 mm,2.1 μm）.The experimental parameters,including-extraction method,compositions of mobile phase and MS parameters were optimized.The results demonstrated that the standard curves were linear in the range of 1.0-100 μmol/L,with correlation coefficients above 0.99.The average recoveries were in the range of 95.7%-101.1%with RSDs of 1.2%-6.1%.The method is sensitive and repeatable,and could be applied in the analysis of intracellular energy metabolism.关键词：UPLC-MS;cellular;ATP;ADP;AMP;NAD+;NADP+
- 2325
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- 1250
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- 3
发布时间： - 摘要：An analytic system of two stages cold trap enrichment and GC/FID for the determination of trace phosphine in environment was developed,using flame ionization detector （FID）.The influences of cold trap temperature,carrier gas flow,column oven temperature and detector temperature on the enrichment and determination for phosphine were investigated.At the same time,the mechanism of phosphine enrichment was also discussed.In addition,the system was used to measure mixture bound phosphine（MBP） in the typical paddy fields in Guangzhou.As a result,the best operation condition was defined as follows： cold trap temperature： -90 ℃,carrier gas flow：1.5 mL/min,column oven temperature： 90 ℃,and detector temperature： 220 ℃.The main mechanism for the cold trap enrichment was the enrichment capillary′s absorption to phosphine,belonging to gas-solid absorption.In the whole cycle of rice growth,the average concentration of mixture bound phosphine （MBP） was 120.59 ng/kg,with an average relative standard deviation（RSD） of 8.5%.With the detection limit of 0.041 7 pg and RSD of 4.3%,this system had a similar function for detecting phosphine to the traditional method with nitrogen phosphorus detector（NPD）.Owing to the simple constitution,easy operation and moderate price of FID,this system makes the analysis of trace phosphine easier,thus could be applied in lad generally.关键词：phosphine;GC/FID;enrichment mechanism
- 1865
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- 1059
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- 0
发布时间： - 摘要：A method was established for the simultaneous determination of seven sulfonylurea hypoglycemic agents（e.g.glipizide，tolbutamide，tolazamide，gliclazide，glibenclamide，glimepiride and glurenor) in health care products by solid phase extraction/high performance liquid chromatography（SPE/HPLC).Various samples were extracted with methanol by ultrasound，the extract was cleaned up and concentrated on an LC-C18 SPE column.The chromatographic separation was performed on an Agilent Zorbax SB-C18 column（250 mm×4.6 mm，5 μm) using acetonitrile（A)-0.02% phosphoric acid solution（B) as mobile phase by gradient elution at a flow rate of 1.0 mL/min，a column temperature of 30 ℃ and an ultraviolet（UV) detection wavelength of 228 nm.The seven sulfonylurea hypoglycemic agents showed good linear relationships in the range of 0.2-20 μg/mL with correlation coefficients larger than 0.999 9.The limits of detection（S/N≥3) were 0.1 mg/kg for seven target compounds.The spiked recoveries of seven target compounds in five different health care products ranged from 81% to 115% with relative standard deviations less than 9%.关键词：solid phase extraction（SPE);high performance liquid chromatography（HPLC);health care products;sulfonylurea hypoglycemic agents
- 1876
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- 1101
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- 7
发布时间： - 摘要：An effective energy transfer could occur between CdS@ZnS QDs and rhodamine B（RhB)in the presence of Tween 40 in pH 7.6 PBS buffer solution,leading to significant fiurensence qunching of CdS@ZnS QDs（donor) and increase of RhB（acceptor).As adding oxytetracycline,the fluorescence intensity of FRET system decreased because the electrostatic interaction between RhB and oxytetracycline occurred.A good linear relationship was observed between fluorescence quenched intensities and concentrations of oxytetracycline in the range of 4×10-8-60×10-8 mol/L,with a correlation coefficient（r) of 0.999 0 and a detection limit of 1.13×10-9 mol/L.Based on this,a new determination method for oxytetracycline was developed.With a good stability and high selectivity,the method was applied in the determination of proteins in milk samples with recoveries of 97.5%-103.7%and relative standard deviations（n=6) not more than 2.0%.关键词：quantum dots;CdS@ZnS;FRET;oxytetracycline
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- 6
发布时间： - 摘要：A liquid chromatography-electrospray ionization-ion trap mass spectrometric（LC-ESI-ITMS） method was develop for the simultaneous analysis of three common analeptics and their in-vitro metabolites.Analeptics were incubated individually with mouse liver microsome S9 fraction firstly，and then the incubation samples were pooled together and analyzed by LC-ESI-ITMS in full-scan mode.Analeptics and their metabolites were identified from the determination of molecular ions.The metabolites detection were confirmed from tandem mass spectrometric（MSn） analysis.With the advanteges of rapidness，high accuracy，good selectivity and sensitivity，the method was suitable for the analysis of three common analeptics and their in-vitro metabolites.关键词：toxicological analysis;liquid chromatography-electrospray ion trap mass spectrometry（LC-ESI-ITMS）;mouse liver S9 fractions;analeptic;in-vitro metabolism
- 1989
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- 992
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- 3
发布时间： - 摘要：Aroma is one of the main factors of rice quality and is important for the evaluation of nutritional value and quality.In order to explore an objective method to evaluate the quality of rice,this paper provided a new possible measure to distinguish the rice of different storage length of time and different original places by gas chromatography（GC) based on head space solid phase micro extraction（HS-SPME) technique and to analyze the volatile components of rice samples with different storage time and original places using principal component analysis（PCA) and partial least squares discriminant analysis（PLS-DA).Results showed that rice samples stored for different time periods were classified correctly in the PCA and PLS plot,cross using the leave one out cross validation（LOO),the prediction accuracy of the samples stored for different time periods was 96%and the relative standard error（RSE) was 8.2%in the model of PLS.Samples from four different original places clustered into four different groups in the PCA plot.The PLS-DA method was applied in the determination of the rice of different original places,which was verified to be very effective and reliable,also the accuracy of this model reached to 100%.It could be safely concluded that this study has provided a reliable and effective method for discriminating the storage time period and original places of rice based on HS-SPME-GC-FID technique combined with PCA and PLS-DA.关键词：rice;solid phase micro extraction（SPME);GC;PCA;PLS-DA
- 2039
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- 972
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- 8
发布时间： - 摘要：A reversed phase high performance liquid chromatographic（RP-HPLC） method was developed for the separation,quanlitative and quantitative analysis of 4-hydroxy butyric acid（GHB）,γ-butyrolactone（GBL） and 1,4-succinic acid in the catalytically oxidative products of tetrahydrofuran（THF）,which was oxydized with molecular oxygen catalyzed by cobalt（Ⅱ） complex.After centrifuged and evaporated,the reaction mixtures were separated on an Inertsil ODS-3 C18 column（250 mm×4.6 mm,5 μm） by isocratic elution with 0.01%formic acid-methanol as mobile phase at a flow rate of 0.80 mL /min.The detection wavelength was set at 220 nm.The identification of three substances were achieved by the retention times and the PAD spectra.Quantification analysis was performed by the external standard method under the optimal conditions.The calibration curves of three compounds were linear in the range of 10.0-1 000 mg/L with correlation coefficients not less than 0.999 8.The detection limits（LODs） were in the range of 0.019-0.052 mg and the quantitation limits（LOQs） were 0.064-0.173 mg.The average recoveries at three spiked levels ranged from 95%to 107%with relative standard deviations（RSDs） of 0.09%-4.7%.The method was applied in the real sample,and the results indicated that the method was simple,sensitive and accurate,and was suitable for the analysis of GHB,GBL and 1,4-succinic in the oxidative products of THF and the evaluation of catalysts.关键词：cobalt（Ⅱ） complex;tetrahydrofuran;reversed phase high performance liquid chroma-tography;γ-butyrolactone
- 2513
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- 1068
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- 1
发布时间： - 摘要：A new method was developed for the detection of ethephon residue in fruits and vegetables by QuEChERS/high performance liquid chromatography-tandem mass spectrometry.The samples were extracted with water，and cleaned up with PSA and GCB.As the chromatography and MS conditions were optimized，the analysis of the target compound was performed under electrospray positive ion source and multiple reaction monitoring mode（MRM），and the quantitation was carried out by the external standard method with the blank matrix.The calibration curve was linear in the range of 0.5-50 mg/L with a correlation coefficient not less than 0.995.The recoveries of ethephon in cucumber，tomato，pepper，apple and banana matrices at three spiked levels of 2.5，5.0，10.0 mg/kg ranged from 80.5% to 92.7%，and the relative standard deviations were in the range of 3.1%-8.3%.The method is simple，fast and safety，and is suitable for the confirmation and quantification of ethephon residue in fruits and vegetables．关键词：QuEChERS;ethephon;HPLC-MS/MS
- 2061
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- 1062
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- 5
发布时间： - 摘要：A new grapheme oxide/thiacalixarene composites material was synthesized.The characterization of this material was performed by SEM，FT-IR，Element analysis and thermal analysis.A new method for the determinaon of trace thallium was developed by graphite furnace atomic absorption spectrometry（GFAAS） with the grapheme oxide/thiacalixarene composites material as adsorbent.The optimal experimental conditions（e.g.pH value of solution，temperature，elution condition） and the adsorbing principle for the determination were studied.It was found that thallium could be adsorbed on grapheme oxide/thiacalixarene composites material at pH 8.0 and temperature of （23±1）℃，and the adsorption capacity was 73.1 mg/g.The determination of trace thallium was performed by GFAAS after it was eluted with 5 mL 0.5 mol/L HCl-1.0 mol/L thiurea.The linear range of thallium ranged from 0.012 μg/L to 15 μg/L.The detection limit（3σ）was 0.008 g/L.The RSD（n=7） for 0.50 μg/L Tl3+ standard solution was 2.3%.The spiked recoveries of thallium were in the range of 93.6%-104.1%.The method was applied in the determination of trace thallium in environmental samples with satisfactory results.关键词：grapheme oxide/thiacalixarene composites material;separation/preconcentration;graphite furnace atomic absorption;thallium
- 2126
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- 1138
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- 8
发布时间： - 摘要：Surface-enhanced Raman scattering（SERS) presents a significant enhanced effect.The surface of Ag electrode was pretreated by electrochemical method to obtain a SERS active substrate.The concentration of salicylic acid（SA) absorbed on this rough Ag electrode could be determined by the corresponding SERS spectra.Potassium thiocyanate was selected as the internal standard and the relative intensity was formed by aromatic ring breathing（1 036 cm-1) of SA and characteristic peak（2 120 cm-1) of SCN-.A good linear relationship was obtained between the relative intensity and concentration of SA in the range of 4×10-6-3×10-4 mol/L，with a correlation coefficient of 0.993 1.The detection limit was 2.04×10-6 mol/L and the recoveries were in the range of 85.4%-114.6%.The interference experiments were also considered.With the advantages of convenience，no interference of internal substance，green and harmlessness，the method could be extended to the determination of other special substances.关键词：surface-enhanced Raman scattering;internal standard method;salicylic acid;silver electrode
- 2171
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- 1090
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- 4
发布时间： - 摘要：A gas chromatography-mass spectrometric（GC-MS） method was developed for the simultaneous determination of polybrominated biphenyl（PBBs） and polybrominated biphenyl ethers（PBDEs） in pressure-sensitive adhesive and its products.The sample was dissolved with ethyl acetate，and then precipited with methanol to remove polymer.The extract was centrifugally separated for 5 min at a speed of 3 000 r/min，and analyzed by GC-MS under the selected ion monitoring mode.The method showed good linearities in the range of 0.1-10 mg/L for PBBs and PBDEs,with correlation coefficients not less than 0.999.The spiked recoveries were in the range of 86.5%-99.7% for PBBs with relative standard deviations（RSDs） less than 7%，and 91.4%-95.8% for PBDEs with RSDs less than 5%.The method was simple，sensitive and repeatable，and could meet the requirements for the determination of PBBs and PBDEs in the pressure-sensitive adhesive and its products.关键词：polybrominated biphenyl（PBBs）;polybrominated biphenyl ethers（PBDEs);pressure-sensitive adhesive;gas chromatography-mass spectrometry（GC-MS）
- 2008
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- 1067
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- 6
发布时间： - 摘要：A new control system was designed for fast gas chromatography(FGC) in this paper.The controller is mainly composed of the column temperature control system，automatic sampling and pressure control system of the gas path.The range of temperature control is from 30 to 160 ℃，the heating rate approximates 3 ℃/s，the temperature control accuracy is about ± 0.5 ℃，and the carrier gas pressure control range is from 0 to 0.5 MPa.Applying the controller in homemade FGC，a standard sample composed of straight-chain n-alkanes(C1-C8) and toluene was tested by the FGC.The result indicated that the 9 species could be completely separated in 100 s.关键词：fast gas chromatography;temperature programming;control system
- 2506
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- 1082
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- 1
发布时间： - 摘要：The γ-Al2O3 was very suitable to be used as a catlalyst support and an adsorbent because of its vast surface.The γ-Al2O3with high surface area was mainly derived from its precursor boehmite （AlOOH）.The porous AlOOH was synthesized by sol-gel method using alumina nitrate and sodium hydroxide as main raw materials.The fasciculate porous alumina was obtained by heat with AlOOH treatment.And it was characterized by XRD,SEM and N2 adsorption-desorption technique.关键词：boehmite;XRD;SEM;N2 adsorption-desorption technique
- 1767
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- 1279
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- 0
发布时间： - 摘要：In order to analyze the change of concentration of main sulfur-contained compounds in different cooking ways,a headspace gas chromatographic method was established and validated for the determination of diallyl sulfide（DAS ）,diallyl disulfide（DADS）,diallyl trisulfide（DATS） in cooked oil.The detection was performed on a DM2WAX capillary column,with an equilibration temperature of 403 K,an equilibration time of 20 min and a rotation rate of 500 r/min.Good linear relationships between peak area and their contents were obtained in the investigated range,with correlation coefficients higher than 0.999 and detection limits of 0.001-0.025 mg/L.The average recoveries of DAS,DADS and DATS were in the range of 97.1%-103%.关键词：garlic;sulfur-contained compound;determination;headspace gas chromatography
- 2135
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- 1048
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- 0
发布时间： - 摘要：Superparamagnetic nanoparticles have been applied increasingly in biochemical analysis due to their super-paramagnetism,good monodispersity,strong magnetic saturation intensity and fast separation speed.They can be used not only as an ideal immune affinity carrier in magnetic separation,but also as a perfect magnetic signal and a signal amplification system in magnetic sensors.The up-dated applications of superparamagnetic nanoparticles in the fields of separation and purification,biosensor,immunodetection,molecular biology and microbiological assay were introduced in this review.The potential of the superparamagnetic nanoparticles in biochemical separation and analysis was also discussed.关键词：superparamagnetic nanoparticle;separation and purification;immunodetection;biosensor;molecular biology
- 2090
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- 1000
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- 4
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