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About Journal

ISSN:1004-4957

CN:44-1318/TH

Administrator: Guangdong Academy of Sciences

Sponsors: Institute of Analysis,Guangdong Academy of Sciences (Guangzhou Center for Analytical and Testing of China) ; China Association for Instrumental Analysis

Editor-in-Chief: Academician Jiang Guibin

Publication Frequency: Monthly

Tel: 020-87684776

Address: No.100, Xianlie Zhong Road, Guangzhou

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Volume 45 期 8，2026 2026年第45卷第8期
      
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    更新时间：2026-09-04

    周铁安

      
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    刘倩

      
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  • Scientific Papers

    ZHANG Qing-qing, LIU Su-ying, DU Cui-cui, ZHANG Xiao-hua, CHEN Jin-hua

    DOI：10.12452/j.fxcsxb.26042501
    摘要：In this study，a dual-mode sensing platform combining photoelectrochemical（PEC） and quartz crystal microbalance（QCM） techniques was developed for highly sensitive assay of protein tyrosine phosphatase 1B（PTP1B） activity. The sensing platform employed a porous anatase/rutile TiO2 heterojunction loaded with phosphotungstic acid（A/R-TiO2@HPW） as the photocurrent-responsive material. The PTP1B-targeted phosphorylated-peptide was immobilized on the QCM gold-coated quartz crystal（Au electrode） through the specific binding between TiO2 and phosphate groups，and silicon dioxide（SiO2） nanospheres were introduced to enhance spatial hindrance，thereby suppressing the initial photocurrent. Upon PTP1B-catalyzed dephosphorylation of the peptide，both the peptide and SiO2 nanospheres were released from the Au electrode，leading to photocurrent recovery along with a significant change in the QCM resonant frequency，thus enabling sensitive PEC-QCM dual-mode detection of PTP1B activity. This strategy integrates PEC and QCM modes and effectively avoids false-positive interference through dual-mode self-calibration. Moreover，by replacing the substrate，the developed sensing platform can be extended into a universal detection platform for protein phosphatases. Given the critical role of PTP1B in insulin signaling and metabolic diseases，this study provides a potentially reliable tool for related pathological research，drug screening，and clinical diagnosis.  
    关键词：protein tyrosine phosphatase;enzyme activity;Photoelectrochemistry;quartz crystal microbalance;dual-mode detection   
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    更新时间：2026-08-29

    CHENG Rui, LIANG Dian-yi, XU Yi-ling, QIU Yu-tong, YIN Kun, YANG Chao-yong

    DOI：10.12452/j.fxcsxb.26040708
    摘要：Single-cell RNA sequencing（scRNA-seq） provides unprecedented resolution for delineating cellular heterogeneity. However，conventional scRNA-seq approaches are bottlenecked by high cell loss rate，multicellular contamination，and high-cost. To address these limitations，we developed Well-Multi-seq，which integrates size exclusion and quasi-static hydrodynamics capture microfluidic chip and cholesterol-modified oligonucleotide tag labeling strategy. This approach enables scRNA-seq with high cell capture efficiency，low contamination，and low-cost. The microfluidic chip overcomes the Poisson distribution limit，achieving high bead（94%） and cell（74%） occupancy and high cell capture rate（87%），thereby significantly improving cell utilization. Leveraging the stable insertion of cholesterol molecules into cell membranes，sample-barcoded oligonucleotide strands are anchored onto the cell surface，allowing one-tube multiplexed sequencing of multiple samples. This strategy not only drastically reduces reagent costs and simplifies experimental workflows but also endows the system with the ability to identify and remove doublet contamination from the library. In mixed human-mouse cell line sequencing，the sample labeling accuracy of this method reaches 92.60%（human） and 95.28%（mouse），demonstrating its superior labeling specificity. Owing to its multiple advantages，Well-Multi-seq is particularly suited for multiplexed analyses of rare cell types such as circulating tumor cells and microneedle biopsy samples，as well as dynamic processes including drug screening and embryonic development，and is poised to become a powerful tool for basic research and clinical diagnosis.  
    关键词：single-cell RNA-sequencing;microfluidic chip;sample multiplexing;cholesterol-modified tag   
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    YI Yi-yi, ZHU Teng-gao, YU Xin-hui, HONG Jie, CHEN Huan-wen

    DOI：10.12452/j.fxcsxb.26041603
    摘要：Traditional mass spectrometers are difficult to be applied directly to field or on-site analysis due to their large size，high power consumption，the need for a high vacuum environment and complex sample pretreatment. Herein，a lightweight，low-power，portable mass spectrometer has been developed，featuring the ability for on-site rapid detection. A small-sized continuous atmospheric pressure interface and a composite ion funnel structure were employed in this mass spectrometer，significantly enhancing the ion transmission efficiency and analytical stability. In terms of the mass-analysis，the sine-wave frequency scanning and quadrupole-enhanced dipole resonance excitation were combined，and a high-sensitivity detection within a wide range of quality has been achieved，while maintaining the resolution of unit mass（FWHM<1 Da）. In normal operation，the weight of the instrument is about 22 kg，with a volume of 44 cm×30 cm×40 cm，and it runs at under 330 W. In the analysis of Chinese medicine，the portable mass spectrometer has shown good application potential，combined with internal extraction electrospray ionization（iEESI） technology. It can realize the in-situ detection and fingerprint construction of volatile components of aromatic Chinese medicine，providing reliable technical support for on-site monitoring and traceability of the quality of traditional Chinese medicine. In summary，by expanding the types of ion sources and integrating the database system，portable mass spectrometers have great potential in the modernization research of traditional Chinese medicine.  
    关键词：portable mass spectrometer;traditional Chinese medicine;rapid detection;quality control;on-site analysis   
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    YANG Hua, SHAO Zi-qi, ZENG Xing-yu, WENG Ya-qing, LI Jia-hui, XIE Qing-ji

    DOI：10.12452/j.fxcsxb.26030301
    摘要：A poly（neutral red）（PNR）/SnO2/FTO photoanode with a PNR/SnO2 type-II heterojunction structure was prepared by cyclic voltammetry electropolymerization of neutral red（NR） on a fluorine-doped tin oxide（FTO） electrode that had been cast-coated with hydrothermally synthesized SnO2. An Apt/GLD/PNR/SnO2/FTO photoanode was prepared after the Schiff base reaction among aminated kanamycin（KAN） aptamer（Apt），glutaraldehyde（GLD），and amino group-bearing PNR，and the signal attenuation photoelectrochemistry analysis of KAN was then realized on the basis of the steric hindrance effect induced by the specific KAN-Apt binding. Under the optimal conditions，the decline value of anodic photocurrent was linearly related to the common logarithm of KAN concentration from 0.01 nmol/L to 750 nmol/L，with a limit of detection（S/N=3） of 3 pmol/L. Satisfactory recoveries were obtained for the analysis of KAN in milk samples.  
    关键词：Photoelectrochemistry;SnO2;poly（neutral red）;aptasensing;steric hindrance effect;kanamycin   
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    更新时间：2026-08-29
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