Advances on Application of Rolling Circle Amplification-mediated Nucleic Acid Signal Amplification Strategy in Biosensors

刘巨; 梁涛波; 许恒毅

doi:doi：10.3969/j.issn.1004-4957.2020.12.019

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Advances on Application of Rolling Circle Amplification-mediated Nucleic Acid Signal Amplification Strategy in Biosensors

更新时间：2023-04-18

- Advances on Application of Rolling Circle Amplification-mediated Nucleic Acid Signal Amplification Strategy in Biosensors
- Vol. 39, Issue 12, Pages: 1556-1560(2020)
- 作者机构：
  
  南昌大学食品科学与技术国家重点实验室
- 作者简介：
- 基金信息：
- DOI：doi：10.3969/j.issn.1004-4957.2020.12.019
  CLC：
- Published：2020
- 稿件说明：
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刘巨, 梁涛波, 许恒毅. Advances on Application of Rolling Circle Amplification-mediated Nucleic Acid Signal Amplification Strategy in Biosensors[J]. 2020, 39(12): 1556-1560.
DOI：

刘巨, 梁涛波, 许恒毅. Advances on Application of Rolling Circle Amplification-mediated Nucleic Acid Signal Amplification Strategy in Biosensors[J]. 2020, 39(12): 1556-1560. DOI： doi：10.3969/j.issn.1004-4957.2020.12.019.

摘要

滚环扩增(RCA)是一种等温核酸扩增技术，因其具有扩增效率高、保真度高等特点，在生物传感器领域得到了广泛的应用。该文介绍了RCA技术的基本原理、RCA环状模板环化方式和RCA的扩增类型，并对基于RCA技术的荧光、比色以及电化学生物传感器进行了介绍，旨在为RCA技术在生物传感器领域的进一步发展和应用提供参考。

Abstract

Rolling circle amplification(RCA) is an isothermal nucleic acid amplification technique.Due to its high efficiency and high fidelity

this technique has been widely used in biosensors.This review introduces the principle of RCA technique

the common cyclization types of RCA template and the common types of RCA

as well as the fluorescent

colorimetric and electrochemical biosensors based on RCA.The aim of this review is to provide references for the further development and application of the RCA technique in biosensors.

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