A high performance liquid chromatography(HPLC) using fused-core silica particles column was developed for the rapid determination 16 local anesthetic components

including procaine

tetracaine

methyl isoeugenol

chloroprocaine hydrochloride

lidocaine hydrochloride

benzocaine

ropivacaine mesylate

butacaine sulphate

procainamide

prilocaine

bupivacaine

benoxinate hydrochloride

eugenol

pramoxine hydrochloride

cinchocaine and methyl eugenol.The samples were ultrasonically extracted with 0.1%hydrochloride-methanol

and separated on an Agilent Poroshell 120 EC-C18(50 mm×4.6 mm×2.7 μm) at 35 ℃ by gradient elution

with dipotassium hydrogen phosphate solution(pH 3.0)-methanol-acetonitrile as mobile phase at a flow rate of 1.5 mL/min.The detection wavelengths for diode array detector were set at 210 nm and 280 nm.Quantification analysis was performed by the external standard method.Results indicated that the calibration curves for the 16 compounds showed good linearity in a certain range of mass concentration with their correlation coefficients not less than 0.999 7.The limits of detection(LOD

S/N=3) and limits of quantitation(LOQ

S/N=10) for ropivacaine mesylate were 3.1 μg/g and 10.2 μg/g

respectively.The LODs and LOQs for other 15 compounds were 1.0 μg/g and 3.3 μg/g

respectively.The average recoveries at three spiked levels ranged from 93.3%to 103%

with relative standard deviations(RSD) of 0.08%-1.2%.The method was rapid and accurate

and could be used for the rapid screening of local anesthetic components in adult products

indicating its extensive application prospect.