Fabrication of Nitrogen Fluoride Co doped Carbon Dots and Their Application in Riboflavin Detection

张丽婷; 高杉; 马榕; 李宁波; 乔洁

doi:doi：10.3969/j.issn.1004-4957.2020.06.010

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Fabrication of Nitrogen Fluoride Co doped Carbon Dots and Their Application in Riboflavin Detection

更新时间：2023-04-18

- Fabrication of Nitrogen Fluoride Co doped Carbon Dots and Their Application in Riboflavin Detection
- Vol. 39, Issue 6, Pages: 762-768(2020)
- 作者机构：
  
  1. 山西医科大学基础医学院生物化学与分子生物学教研室
  2. 山西医科大学基础医学院化学教研室
- 作者简介：
- 基金信息：
- DOI：doi：10.3969/j.issn.1004-4957.2020.06.010
  CLC：
- Published：2020
- 稿件说明：
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张丽婷, 高杉, 马榕, 李宁波, 乔洁. Fabrication of Nitrogen Fluoride Co doped Carbon Dots and Their Application in Riboflavin Detection[J]. 2020, 39(6): 762-768.
DOI：

张丽婷, 高杉, 马榕, 李宁波, 乔洁. Fabrication of Nitrogen Fluoride Co doped Carbon Dots and Their Application in Riboflavin Detection[J]. 2020, 39(6): 762-768. DOI： doi：10.3969/j.issn.1004-4957.2020.06.010.

摘要

以柠檬酸、L-赖氨酸和氟化钠为原料，利用水热法制备了具有明亮蓝色荧光的氮氟共掺杂碳点(NFCDs)，通过透射电子显微镜(TEM)、傅立叶变换红外光谱(FT-IR)、紫外-可见吸收光谱(UV-Vis)和荧光光谱对合成的NFCDs进行表征，并基于核黄素与NFCDs之间的荧光共振能量转移构建了NFCDs荧光探针用于核黄素的检测。在优化实验条件下，该方法对核黄素的线性范围为 30~66.5 μmol/L，检出限为0.26 μmol/L，将其用于牛奶和奶粉中核黄素的测定，回收率为96.7%~103%。经MTT法测定NFCDs对HeLa细胞为低毒性，并成功用于细胞中核黄素的检测。

Abstract

Nitrogen fluoride co-doped carbon dots(NFCDs) were synthesized by hydrothermal method using citric acid

L-lysine and sodium fluoride as raw material.The structures of carbon dots were characterized by transmission electron microscopy(TEM)

Fourier transform infrared spectroscopy(FT-IR)

ultraviolet visible spectroscopy(UV-Vis) and fluorescence spectroscopy.Based on the fluorescence resonance energy transfer between riboflavin and NFCDs

a NFCDs fluorescent probe was constructed for the detection of riboflavin.Under the optimized experimental conditions

the detection linearity of this method for riboflavin ranged from 3.0 μmol/L to 66.5 μmol/L with a detection limit of 0.26 μmol/L

calculated by 3 times of the signal-to-noise ratio.The probe was successfully applied to the determination of riboflavin in milk and milk powder with recoveries of 96.7%-103%.In addition

the low toxicity of NFCDs on HeLa cells was determined by the MTT assay

and they were also applied to fluorescence imaging of cells

and the presence of riboflavin in cells was successfully confirmed by laser scanning confocal microscopy.

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