Detection of Citric Acid in Urine Using a Hydroxypyridine Functionalized Spiropyran Probe

霍志铭; 何汶懿; 王婕; 夏凌; 肖小华; 李攻科

doi:doi：10.3969/j.issn.1004-4957.2020.05.005

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Detection of Citric Acid in Urine Using a Hydroxypyridine Functionalized Spiropyran Probe

更新时间：2023-04-18

- Detection of Citric Acid in Urine Using a Hydroxypyridine Functionalized Spiropyran Probe
- Vol. 39, Issue 5, Pages: 590-596(2020)
- 作者机构：
  
  中山大学化学学院
- 作者简介：
- 基金信息：
- DOI：doi：10.3969/j.issn.1004-4957.2020.05.005
  CLC：
- Published：2020
- 稿件说明：
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霍志铭, 何汶懿, 王婕, 夏凌, 肖小华, 李攻科. Detection of Citric Acid in Urine Using a Hydroxypyridine Functionalized Spiropyran Probe[J]. 2020, 39(5): 590-596.
DOI：

霍志铭, 何汶懿, 王婕, 夏凌, 肖小华, 李攻科. Detection of Citric Acid in Urine Using a Hydroxypyridine Functionalized Spiropyran Probe[J]. 2020, 39(5): 590-596. DOI： doi：10.3969/j.issn.1004-4957.2020.05.005.

摘要

柠檬酸是多种疾病潜在的生物标记物，因此发展对柠檬酸灵敏、专一的快速检测方法势在必行。该文设计合成了一种羟基吡啶功能化水溶性螺吡喃探针，通过在螺吡喃骨架上引入羟基吡啶基团，利用螺吡喃螺碳及羟基的协同作用增强了探针对柠檬酸的结合能力。结果表明，该探针对柠檬酸有选择性响应，并伴随着明显的颜色变化及荧光猝灭。据此建立了尿样中柠檬酸的荧光检测方法，方法线性范围为20~120 μmol/L，检出限为1.0 μmol/L。人工尿液中柠檬酸的回收率为85.7%~92.2%，相对标准偏差为3.0%~9.2%。该方法具有操作简便、准确及选择性好的优点，在尿样柠檬酸检测中具有良好的应用潜力。

Abstract

To develop a rapid and highly selective method for detection of citric acid is a must as citric acid is an important marker for a variety of diseases.A water soluble hydroxypyridine functionalized spiropyran probe was designed and synthesized by introducing hydroxypyridine group into spiropyran skeleton

as to improve its binding ability to citric acid through the synergy effect between spiropyran carbon and hydroxyl group.Results showed that the probe responded to citric acid with high selectivity

accompanying with significant color change and fluorescence quenching.Based on this

a rapid and sensitive fluorescence method for detection of citric acid was established.The probe was applied in the detection of citric acid in urine sample

with a linear range of 20-120 μmol/L and a detection limit of 1.0 μmol/L.Moreover

the probe was used for the detection of citric acid in 2.5% artificial urine.The recoveries ranged from 85.7% to 92.2%

with relation standard deviations of 3.0%-9.2%.The method is facile

accurate and selective

and is potential in the determination of citric acid in urine.

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