Fabrication of PSMA@Ag Composite Microbeads and Their Application in Small Molecular Analysis Based on MALDI-MS Platform

谢斌; 李万万

doi:doi：10.3969/j.issn.1004-4957.2020.03.001

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Fabrication of PSMA@Ag Composite Microbeads and Their Application in Small Molecular Analysis Based on MALDI-MS Platform

更新时间：2023-05-25

- Fabrication of PSMA@Ag Composite Microbeads and Their Application in Small Molecular Analysis Based on MALDI-MS Platform
- Vol. 39, Issue 3, Pages: 295-300(2020)
- 作者机构：
  
  上海交通大学材料科学与工程学院
- 作者简介：
- 基金信息：
- DOI：doi：10.3969/j.issn.1004-4957.2020.03.001
  CLC：
- Published：2020
- 稿件说明：
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谢斌, 李万万. Fabrication of PSMA@Ag Composite Microbeads and Their Application in Small Molecular Analysis Based on MALDI-MS Platform[J]. 2020, 39(3): 295-300.
DOI：

谢斌, 李万万. Fabrication of PSMA@Ag Composite Microbeads and Their Application in Small Molecular Analysis Based on MALDI-MS Platform[J]. 2020, 39(3): 295-300. DOI： doi：10.3969/j.issn.1004-4957.2020.03.001.

摘要

基质辅助激光解吸电离质谱(MALDI-MS)作为一种有力的分析手段，在生物分子分析中有着广泛的应用，但很难应用于分子量小于500的待测物的分析。该文利用聚多巴胺修饰还原法制备了核壳结构的聚苯乙烯-马来酸酐共聚物@银纳米壳层(PSMA@Ag)复合微球。采用傅立叶红外光谱法验证了聚多巴胺(PDA)的成功修饰。结合扫描电子显微镜(SEM)和紫外-可见光谱(UV-Vis)分析结果，发现Ag纳米壳层成功地包覆在PSMA微球的表面。将制备的PSMA@Ag复合微球作为辅助基质直接应用于MALDI-MS，成功地从0.5 μL待测物样品中检测到2 pmol脯氨酸(Mw=115)和1 pmol丝氨酸(Mw=105)。研究结果证明PSMA@Ag微球对MALDI的离子化过程有促进作用，为解决MALDI-MS在分析小分子待测物时背景噪声过大，信号无法分辨的问题提供了一个有效途径。

Abstract

As a powerful analysis approach

matrix assisted laser desorption/ionization mass spectrometry(MALDI-MS) has attracted considerable attention in biomolecular analysis.However

this approach could hardly be used in the detection for samples with molecular mass lower than 500.In this work

poly (styrene-co-maleic anhydride) microbead@silver nanoshell(PSMA@Ag) composite microbeads with core-shell structure were synthesized using a polydopamine modification method.FT-IR results demonstrated the successful coating of polydopamine(PDA).SEM images along with UV-Vis results indicated that Ag nanoparticles were uniformly modified onto the surface of PSMA microbeads.The prepared PSMA@Ag microbeads were directly applied in matrix assisted laser desorption/ionization mass spectrometry

successfully detecting 2 pmol proline (Mw=115) and 1 pmol serine(Mw=105) in 0.5 μL sample

respectively.This work verified the positive effect of PSMA@Ag microbeads in MALDI-MS laser desorption/ionization process

and provided a promising route for MALDI analysis of the biomolecules with low molecular mass.

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