Rapid and Visual Inspection of Four Organophosphorus Pesticides in Medicinal Materials Using Nanogold Based Nucleic Acid Aptamers

刘媛媛; 陈媛媛; 张红艳; 尤金坤; 魏小红; 余宇燕

doi:doi：10.3969/j.issn.1004-4957.2019.12.018

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Rapid and Visual Inspection of Four Organophosphorus Pesticides in Medicinal Materials Using Nanogold Based Nucleic Acid Aptamers

- Rapid and Visual Inspection of Four Organophosphorus Pesticides in Medicinal Materials Using Nanogold Based Nucleic Acid Aptamers
- Vol. 38, Issue 12, Pages: 1511-1515(2019)
- 作者机构：
  
  福建中医药大学药学院
- 作者简介：
- 基金信息：
- DOI：doi：10.3969/j.issn.1004-4957.2019.12.018
  CLC：
- Published：2019
- 稿件说明：
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刘媛媛, 陈媛媛, 张红艳, 尤金坤, 魏小红, 余宇燕. Rapid and Visual Inspection of Four Organophosphorus Pesticides in Medicinal Materials Using Nanogold Based Nucleic Acid Aptamers[J]. 2019, 38(12): 1511-1515.
DOI：

刘媛媛, 陈媛媛, 张红艳, 尤金坤, 魏小红, 余宇燕. Rapid and Visual Inspection of Four Organophosphorus Pesticides in Medicinal Materials Using Nanogold Based Nucleic Acid Aptamers[J]. 2019, 38(12): 1511-1515. DOI： doi：10.3969/j.issn.1004-4957.2019.12.018.

摘要

建立了一种基于金纳米粒子-核酸适配体的可视化纳米生物传感器检测中药材中有机磷的方法。选用可与有机磷农药特异性结合的广谱适配体作为识别元件，以纳米金为信号转导元件，在纳米金粒子表面修饰有机磷适配体，构建出能够检测甲拌磷、氧化乐果、丙溴磷、水胺硫磷4种有机磷农药的适配体传感器，并将其用于中药材中４种有机磷农药的检测。结果显示，当4种有机磷残留总量超过1 000 μg/L时，溶液体系由红色变为蓝色，可达到肉眼快速检测的效果。该方法操作简单、特异性好、检测结果直观，适用于中药材中4种有机磷农药的现场快速检测。

Abstract

A method of gold nanoparticle based nucleic acid aptamers was developed for the visual inspection of organic phosphorus in Chinese herbal medicines.The nucleic acid aptamers were fabricated by using a broad spectrum aptamer specifically binding to specific organophosphorus pesticide as recognition element and nano gold particles as signal transduction element

immobilizing the former on the surface of the latter to construct four organic substances.Therefore

an organophosphorus aptamer was constructed for the multi residue detection of organophosphorus in Chinese herbal medicines.The solution system changes from red to blue as the residual amount of four organic phosphorus exceeds 1 000 μg/L

which could achieve the effect of rapid detection with the naked eye.With simple operation

good specificity and intuitive detection

the method is suitable for on site rapid detection of four organophosphorus pesticides in Chinese herbal medicines.

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