Detection of Acetylcholinesterase Using a Turn-on Fluorescence Probe Based on Polydopamine Nanoparticles

杜方凯; 李梦汝; 莫远健; 谭学才; 黄乃阳; 黄安娜

doi:doi：10.3969/j.issn.1004-4957.2019.11.010

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Detection of Acetylcholinesterase Using a Turn-on Fluorescence Probe Based on Polydopamine Nanoparticles

- Detection of Acetylcholinesterase Using a Turn-on Fluorescence Probe Based on Polydopamine Nanoparticles
- Vol. 38, Issue 11, Pages: 1347-1352(2019)
- 作者机构：
  
  1. 广西民族大学化学化工学院
  2. 广西高校食品安全与药物分析化学重点实验室
  3. 广西林产化学与工程重点实验室
- 作者简介：
- 基金信息：
- DOI：doi：10.3969/j.issn.1004-4957.2019.11.010
  CLC：
- Published：2019
- 稿件说明：
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杜方凯, 李梦汝, 莫远健, 谭学才, 黄乃阳, 黄安娜. Detection of Acetylcholinesterase Using a Turn-on Fluorescence Probe Based on Polydopamine Nanoparticles[J]. 2019, 38(11): 1347-1352.
DOI：

杜方凯, 李梦汝, 莫远健, 谭学才, 黄乃阳, 黄安娜. Detection of Acetylcholinesterase Using a Turn-on Fluorescence Probe Based on Polydopamine Nanoparticles[J]. 2019, 38(11): 1347-1352. DOI： doi：10.3969/j.issn.1004-4957.2019.11.010.

摘要

以多巴胺盐酸盐为原料，在碱性条件下通过氧化反应制备聚多巴胺荧光纳米粒子(F-PDA)，再与二氧化锰(MnO2)纳米片进行复合，构建了用于检测乙酰胆碱酶(AChE)的F-PDA@MnO2复合物荧光探针。MnO2纳米片和F-PDA复合，体系的荧光被猝灭。在底物乙酰硫代胆碱(ATCh)存在下，加入AChE后，体系荧光恢复，恢复程度与AChE浓度在5.0~100 mU/mL范围内呈良好的线性关系，检出限为0.14 mU/mL(S/N=3)。该方法用于缓冲溶液中AChE的检测，加标回收率为89.5%~120%，相对标准偏差为1.6%~2.5%，且具有较高的选择性。可为基于F-PDA传感体系构建提供新的方法学模型。

Abstract

Polydopamine fluorescent nanoparticles(F-PDA) were prepared with dopamine hydrochloride by oxidation under alkaline conditions.The F-PDA@MnO2 complex was prepared by combining F-PDA with manganese dioxide(MnO2) nanosheets.Based on this

a fluorescent probe based on F-PDA@MnO2 complex was constructed for the detection of acetylcholinesterase(AChE) as the fluorescence of F-PDA could be quenched by adding MnO2 nanosheets.In the presence of acetylthiocholine iodide(ATCh)

the fluorescence of F-PDA was restored after adding AChE.Results showed that there was a good linear relationship for fluorescence recovery of the probe with AChE in the concentration range of 5-100 mU/mL

and the detection limit(S/N=3) was 014 mU/mL.The probe was applied in the detection of AChE in buffer solution with high selectivity.The recoveries were in the range of 89.5%-120%with the relative standard deviations(RSD) of 1.6%-2.5%.The research results provide a new methodological model for the construction of sensing system based on F-PDA.

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