A high performance liquid chromatographic method was developed for the simultaneous determination of 11 benzimidazoles metabolites residues in fat.Samples were firstly dissolved with n hexane

then extracted with antioxidant and 0.1 mol/L hydrochloric acid-acetonitrile(1∶1

by volume)

followed by treatments with n hexane for defatting

antioxidant again and further clean-up on a MCX solid phase extraction(SPE) column.After concentration

the chromatographic separation was performed on an Atlantis T3 column by gradient elution with a mixed solution of methanol and 1%acetic acid at a flow rate of 1.0 mL/min.The detection wavelength was set at 292 nm

and the external standard method was used for quantitation.The calibration curves for 11 analytes were linear in the range of 25.0-1 000 μg/L with quantitation limits of 50 μg/kg.The recoveries at three spiked levels ranged from 82.0%to 109%with relative standard deviations(RSD) of 0.71%-9.9%.The developed method could meet the requirements for determination of 11 benzimidazoles metabolites residues in fat.