Development of a Disposable Paper-based Plate for Identification of Microorganism by MALDI-TOF MS

曾真; 喻佳俊; 刘平; 黄正旭; 高伟; 李梅; 周振; 李磊

doi:doi：10.3969/j.issn.1004-4957.2019.06.004

您当前的位置：

首页 >

文章列表页 >

Development of a Disposable Paper-based Plate for Identification of Microorganism by MALDI-TOF MS

- Development of a Disposable Paper-based Plate for Identification of Microorganism by MALDI-TOF MS
- Vol. 38, Issue 6, Pages: 655-660(2019)
- 作者机构：
  
  1. 暨南大学质谱仪器与大气环境研究所
  2. 广州禾信康源医疗科技有限公司
- 作者简介：
- 基金信息：
- DOI：doi：10.3969/j.issn.1004-4957.2019.06.004
  CLC：
- Published：2019
- 稿件说明：
移动端阅览
曾真, 喻佳俊, 刘平, 黄正旭, 高伟, 李梅, 周振, 李磊. Development of a Disposable Paper-based Plate for Identification of Microorganism by MALDI-TOF MS[J]. 2019, 38(6): 655-660.
DOI：

曾真, 喻佳俊, 刘平, 黄正旭, 高伟, 李梅, 周振, 李磊. Development of a Disposable Paper-based Plate for Identification of Microorganism by MALDI-TOF MS[J]. 2019, 38(6): 655-660. DOI： doi：10.3969/j.issn.1004-4957.2019.06.004.

摘要

针对基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)目前使用的不锈钢靶板或一次性可抛弃塑料靶板存在的清洗流程复杂，易有残留从而产生交叉污染或需要回收、污染环境等问题，开发了一种可一次性使用的纸基靶板。该靶板成本低，使用后方便处理，不会对环境造成污染。并利用标准品从分辨率、灵敏度、重现性、微生物鉴定4个方面对纸基靶板进行了测试，结果表明开发的纸基靶板在分辨率、灵敏度等指标方面与钢靶板基本一致，样品检测重现性较好，具有应用于临床微生物检测的潜力。

Abstract

A disposable paper-based plate was developed for the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer(MALDI-TOF MS) as the existing stainless steel plate or disposable plastic plate requires a complicated cleaning process

which is easy to generate residues causing cross-contamination.Besides

it is not environmentally-friendly when these plates are recycled.Being low-cost and easy to handle after employment

the plate makes no pollution to the environment.The paper-based plate was tested in four aspects

i.e.resolution

sensitivity

reproducibility and microorganism identification.Results showed that the paper-based plate is basically consistent with the steel plate in terms of resolution and sensitivity

and it has a potential in the clinical identification of microorganism with good reproducibility.

关键词

Keywords

references

Views

193

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Automatic Detection of Microplastics Based on Deep Learning Object Detection Combined with Micro-Raman Spectroscopy

Comparison of Performance and Applicability Analysis of μ-FTIR and Py-GC-MS for the Detection of Microplastics in Airborne Dust

Spatial-Temporal Distribution and Ecological Risk Assessment of Marine Microplastics in Macao

Lipid Interference in Pyrolysis-Gas Chromatography-Mass Spectrometry Detection of Microplastics：Problem Identification，Validation Approaches，and Mitigation Strategies

Non-targeted Screening Analysis of Biodegradable Plastics Components Based on Chromatography-Mass Spectrometry

Related Author

No data

Related Institution

No data

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
Total Visits：942211

⁰