Preparation of Magnetic Nanomolecularly Imprinted Polymers and Their Application in Investigation of Trace PQQ-DA Products in Organisms

毛师师; 周杏琴; 钦晓峰; 徐希杰; 谢敏浩

doi:doi：10.3969/j.issn.1004-4957.2019.04.018

您当前的位置：

首页 >

文章列表页 >

Preparation of Magnetic Nanomolecularly Imprinted Polymers and Their Application in Investigation of Trace PQQ-DA Products in Organisms

- Preparation of Magnetic Nanomolecularly Imprinted Polymers and Their Application in Investigation of Trace PQQ-DA Products in Organisms
- Vol. 38, Issue 4, Pages: 482-487(2019)
- 作者机构：
  
  1. 江苏省原子医学研究所
  2. 卫生部核医学重点实验室
  3. 江苏省分子核医学重点实验室
- 作者简介：
- 基金信息：
- DOI：doi：10.3969/j.issn.1004-4957.2019.04.018
  CLC：
- Published：2019
- 稿件说明：
移动端阅览
毛师师, 周杏琴, 钦晓峰, 徐希杰, 谢敏浩. Preparation of Magnetic Nanomolecularly Imprinted Polymers and Their Application in Investigation of Trace PQQ-DA Products in Organisms[J]. 2019, 38(4): 482-487.
DOI：

毛师师, 周杏琴, 钦晓峰, 徐希杰, 谢敏浩. Preparation of Magnetic Nanomolecularly Imprinted Polymers and Their Application in Investigation of Trace PQQ-DA Products in Organisms[J]. 2019, 38(4): 482-487. DOI： doi：10.3969/j.issn.1004-4957.2019.04.018.

摘要

以磁性Fe3O4为内核，对磁性纳米粒子表面进行包裹和修饰，制备了对吡咯并喹啉醌-多巴胺（PQQ-DA）具有高识别性、高选择性的磁性纳米分子印迹材料，对复杂基质的脑组织中低浓度化合物的分离、净化和富集具有很大优势。并结合超高效液相色谱-质谱（UPLC-MS）测定了生物体内PQQ-DA的含量。结果显示，PQQ-DA在0.02~0.1 mg/mL范围内呈良好的线性关系，相关系数（r2）为0.996 6，检出限为0.2×10-11 mg/mL，加标回收率为88.7%~95.5%，相对标准偏差为2.6%~3.2%。该方法灵敏度高、重复性好，可用于生物体内PQQ-DA痕量产物的检测。

Abstract

A pyrroloquinoquinone（PQQ）-dopamine（DA）magnetic nanoparticle imprinted polymers were prepared by the combination of PQQ-DA and magnetic nano Fe3O4 as the core wrapped with SiO2

which possess high recognition and high selectivity toward PQQ-DA and have great advantages in the separation

purification and enrichment of low concentration compounds in complex matrix of brain tissue after molecular imprinting modification.The magnetic nanoparticles were applied in the determination of trace PQQ-DA in brain tissue samples by ultrahigh performance liquid chromatography-tandem mass spectrometry（UPLC-MS）.Results showed that

under the optimized conditions

there was a good linear relationship for PQQ-DA in the range of 0.02-0.1 mg/mL with a correlation coefficient（r2）of 0.996 6

and the detection limit was 0.2×10-11 mg/mL.The recoveries for brain tissue samples were in the range of 887%-955%

with relative standard deviations（n=6）of 2.6%-3.2%.The method could be used in the detection of trace PQQ-DA in vivo with high sensitivity and good repeatability.

关键词

Keywords

references

Views

224

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Determination of 9 Alkylphenols and Alkylphenol Ethoxylates in Down by High Performance Liquid Chromatography-Tandem Mass Spectrometry Combined with Solid Phase Extraction

Simultaneous Determination of SpiramycinⅠand Its Metabolite Residues in Livestock and Poultry Tissues and Milk by HPLC-MS/MS

Application of Molecularly Imprinted Covalent Organic Frameworks in Environmental Sample Pretreatment

Release，Migration and Accumulation Characteristics of UV Absorbents in Biodegradable Mulch Film in the Soil-Maize System

Effect of Typical Polysaccharide and Glycolipid Extracellular Polymeric Substances on Adsorption Behavior of Arsenic onto Microplastics

Related Author

LING Ting

LI Hui-ling

ZHANG Na

PAN Li-ping

SUN Shi-yuan

LIU Jia-li

YAN Wei

ZHU Ping-ping

Related Institution

Jiaxing Institute for Food，Drug and Product Quality Control

National Quality Inspection and Testing Center of Garment Accessories

Food Inspection and Quarantine Technology Center of Shenzhen Customs

School of Food and Drug，Shenzhen Polytechnic University

Hangzhou Xinxuan Network Technology Co.，Ltd

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
Total Visits：942211

⁰