Microcystin（MC）

a type of small molecule polypeptide biotoxin produced by algal blooms

is high toxic to humans and animals

and has been classified as a potential human carcinogen（Group 2B）.Algal blooms break out more and more frequently and tempestuously due to the large discharge of worldwide industrial and domestic wastewater and the climate warming worldwide

which caused the massive accumulation of MCs in water-bodies and soil

threatening the ecological environment and affecting the quality and safety of agricultural products.The immunoassay based on “antibody-antigen” recognition principle is a hot spot research in the rapid determination of biotoxins.The antibody preparation technology is developing in leaps and bounds in the late years

and now has been developed from traditional polyclonal antibody and monoclonal antibody to novel genetic engineering artificial antibody.The internal and external factors of microcystin production

and major hazards toward ecosystem

humans and animals are expounded systematically in the present paper

and the latest research progress of novel artificial antibody preparation technologies and their applications in MC-LR determination are combed comprehensively.Finally

combined with our latest research results

the development trend

existing problems and solutions of novel artificial antibodies in MC-LR determination are further discussed.