Synthesis of Porous Porphyrin Compound and Its Application in Photoelectric Immunosensor

王敏; 孟爽; 谷梦巧; 迟宽能; 马玉婵; 邓燕; 李德蕾; 张茜; 胡蓉; 杨云慧

doi:doi：10.3969/j.issn.1004-4957.2019.03.004

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Synthesis of Porous Porphyrin Compound and Its Application in Photoelectric Immunosensor

- Synthesis of Porous Porphyrin Compound and Its Application in Photoelectric Immunosensor
- Vol. 38, Issue 3, Pages: 283-288(2019)
- 作者机构：
  
  云南师范大学化学化工学院
- 作者简介：
- 基金信息：
- DOI：doi：10.3969/j.issn.1004-4957.2019.03.004
  CLC：
- Published：2019
- 稿件说明：
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王敏, 孟爽, 谷梦巧, 迟宽能, 马玉婵, 邓燕, 李德蕾, 张茜, 胡蓉, 杨云慧. Synthesis of Porous Porphyrin Compound and Its Application in Photoelectric Immunosensor[J]. 2019, 38(3): 283-288.
DOI：

王敏, 孟爽, 谷梦巧, 迟宽能, 马玉婵, 邓燕, 李德蕾, 张茜, 胡蓉, 杨云慧. Synthesis of Porous Porphyrin Compound and Its Application in Photoelectric Immunosensor[J]. 2019, 38(3): 283-288. DOI： doi：10.3969/j.issn.1004-4957.2019.03.004.

摘要

构建了以卟啉多孔化合物-金纳米颗粒（Au NPs@PAF）为固定基质的无标记型C-反应蛋白（CRP）光电免疫传感器。在最优条件下，采用计时电流法实现了对CRP的定量检测。该传感器在CRP质量浓度0.05 ~60 ng/mL范围内与光电流有较好的线性关系，检出限为0.017 ng/mL，相关系数为0.994 6，平均回收率为102%，具有良好的选择性，为C-反应蛋白的检测提供了一种灵敏的方法。

Abstract

In this paper

a label free C-reactive protein（CRP）photoelectricimmunosensor was constructed using porous porphyrin compound-Au nanoparticles（Au NPs@PAF）as an immobilized substrate.Under the optimal conditions

the CRP was quantitatively detected by chronograph current method.There was a good linear relationship for the sensor versus photocurrent in the range of 0.05-60 ng/mL

with a linear correlation coefficient of 0.994 6.The limit of detection was 0.017 ng/mL

and the average recovery was 102%.This sensor provided a sensitive method for the detection of C-reactive protein with good selectivity.

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