Study on Detection of DNA by Laser Raman Spectrometry

李振庆; 何志祥; 尚华; 张大伟; 山口佳则

doi:doi：10.3969/j.issn.1004-4957.2019.02.018

您当前的位置：

首页 >

文章列表页 >

Study on Detection of DNA by Laser Raman Spectrometry

更新时间：2023-04-18

- Study on Detection of DNA by Laser Raman Spectrometry
- Vol. 38, Issue 2, Pages: 235-239(2019)
- 作者机构：
  
  1. 上海理工大学光电信息与计算机工程学院
  2. 教育部医用光学仪器与设备重点实验室
  3. 开封市儿童医院
  4. 大阪大学先进光子研究中心
- 作者简介：
- 基金信息：
- DOI：doi：10.3969/j.issn.1004-4957.2019.02.018
  CLC：
- Published：2019
- 稿件说明：
移动端阅览
李振庆, 何志祥, 尚华, 张大伟, 山口佳则. Study on Detection of DNA by Laser Raman Spectrometry[J]. 2019, 38(2): 235-239.
DOI：

李振庆, 何志祥, 尚华, 张大伟, 山口佳则. Study on Detection of DNA by Laser Raman Spectrometry[J]. 2019, 38(2): 235-239. DOI： doi：10.3969/j.issn.1004-4957.2019.02.018.

摘要

以激光拉曼光谱仪采集腺嘌呤(A)、鸟嘌呤(G)、胞嘧啶(C)、胸腺嘧啶(T)的拉曼光谱图，分析了各碱基谱峰归属，并依据归属结果检测混合碱基组成。结果显示：4种碱基的特征峰集中在175~1 800 cm-1。相同积分时间和激光功率条件下，该波段内嘌呤碱基的激光拉曼光谱峰高于嘧啶碱基的谱峰。腺嘌呤、鸟嘌呤、胞嘧啶、胸腺嘧啶分别在723、648、791、1 368 cm-1处的谱峰最强，利用这些特征峰能在2 min内鉴别出混合碱基中的各碱基。

Abstract

A method of laser Raman spectrometry was developed for the detection of DNA bases.The Raman spectra of adenine(A)

guanine(G)

cytosine(C) and thymine(T) were collected with laser Raman spectrometer in this work.The characteristic peaks in the spectra for each DNA base were analyzed

which were used to determine the components of DNA base mixtures.Results demonstrated that the characteristic peaks mainly ranged from 175 cm-1 to 1 800 cm-1.The peaks of A and G were higher than those of C and T when the integration time and laser power were certain.The strongest Raman signals for A

and T are at 723

648

791 and 1 368 cm-1

respectively.Each DNA base could be determined by using the characteristic peaks from the mixed DNA bases within 2 min.

关键词

Keywords

references

Views

304

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Optimization of Nile Red Staining Method and Application of HSV Color Model in Microplastic Detection and Quantification

A Novel Toxicity Analysis Method for Oxidative Damage Based on Normalized Physical Characteristic Exposure to Microplastics

Effect of Typical Polysaccharide and Glycolipid Extracellular Polymeric Substances on Adsorption Behavior of Arsenic onto Microplastics

Spatial-Temporal Distribution and Ecological Risk Assessment of Marine Microplastics in Macao

Non-targeted Screening Analysis of Biodegradable Plastics Components Based on Chromatography-Mass Spectrometry

Related Author

No data

Related Institution

No data

Address：100 Xianlie Middle Road, Guangzhou Postal code：510070
Tel：020-87684776,37656606 Fax：020-87684776 Email：fxcsxb@china.com
Technical support is provided by Beijing Founder electronics co., LTD 粤ICP备12017312号-3 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
Total Visits：942211

⁰