Identification of Chemical Components in White Tea and Pu’er Raw Tea by Ultra-high Performance Liquid Chromatography－Quadrupole－Orbitrap High-resolution Mass Spectrometry

LIU Yi-jing; LÜ Yue-guang; HU Qi; WANG Guang-min; MA Tao; WANG Peng-long; MA Qiang; LEI Hai-min

doi:10.19969/j.fxcsxb.23020101

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Identification of Chemical Components in White Tea and Pu’er Raw Tea by Ultra-high Performance Liquid Chromatography－Quadrupole－Orbitrap High-resolution Mass Spectrometry

Scientific Papers | 更新时间：2023-05-15

- Identification of Chemical Components in White Tea and Pu’er Raw Tea by Ultra-high Performance Liquid Chromatography－Quadrupole－Orbitrap High-resolution Mass Spectrometry
- Journal of Instrumental Analysis Vol. 42, Issue 5, Pages: 586-595(2023)
- 作者机构：
  
  1.北京中医药大学中药学院，北京 102488
  2.中国检验检疫科学研究院，北京 100176
  3.大连理工大学化工学院药学系，辽宁大连 116024
  4.双柏益康商贸有限公司，云南楚雄 675107
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.23020101
  CLC：
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刘艺静,吕悦广,胡琪等.超高效液相色谱－四极杆－静电场轨道阱高分辨质谱法鉴定白茶和普洱生茶中的化学成分[J].分析测试学报,2023,42(05):586-595.

LIU Yi-jing,LÜ Yue-guang,HU Qi,et al.Identification of Chemical Components in White Tea and Pu’er Raw Tea by Ultra-high Performance Liquid Chromatography－Quadrupole－Orbitrap High-resolution Mass Spectrometry[J].Journal of Instrumental Analysis,2023,42(05):586-595.
刘艺静,吕悦广,胡琪等.超高效液相色谱－四极杆－静电场轨道阱高分辨质谱法鉴定白茶和普洱生茶中的化学成分[J].分析测试学报,2023,42(05):586-595. DOI： 10.19969/j.fxcsxb.23020101.

LIU Yi-jing,LÜ Yue-guang,HU Qi,et al.Identification of Chemical Components in White Tea and Pu’er Raw Tea by Ultra-high Performance Liquid Chromatography－Quadrupole－Orbitrap High-resolution Mass Spectrometry[J].Journal of Instrumental Analysis,2023,42(05):586-595. DOI： 10.19969/j.fxcsxb.23020101.

摘要

基于超高效液相色谱－四极杆－静电场轨道阱高分辨质谱（UHPLC－Q－Orbitrap－HRMS）技术，建立了白茶和普洱生茶中化学成分的分析方法。白茶和普洱生茶提取液经Waters ACQUITY UPLC BEH C,18,柱（150 mm × 2.1 mm，1.7 μm）分离，进入四极杆－静电场轨道阱高分辨质谱，在电喷雾正、负电离模式下同时进行全扫描以及数据依赖型二级质谱扫描并采集数据信息。通过一级质谱分析和二级碎片的精确质量数信息及在线数据库匹配，在白茶和普洱生茶样本中共鉴定出64种活性成分、32种共有活性成分。在白茶和普洱生茶样本中各单独鉴定出16种活性成分，主要为氨基酸类、有机酸类、黄酮类、香豆素类、生物碱类以及核苷类等六大类别化合物。采用归一化法确定已检出的64种活性成分的相对含量。该方法可系统分析茶叶中的有效成分，为进一步研究白茶和普洱生茶的保健机理提供了科学依据，也可为茶叶质量安全监管提供技术支撑。

Abstract

A rapid analytical method for detection of chemical components in white tea and Pu’er raw tea was established based on ultra-high performance liquid chromatography－quadrupole－Orbitrap high-resolution mass spectrometry（UHPLC－Q－Orbitrap－HRMS） in this paper．The tea extracts were separated on a Waters ACQUITY UPLC BEH C,18, column（150 mm × 2.1 mm，1.7 μm），and then analyzed by Q－Orbitrap－HRMS．Full scan/dd－MS,2, was used in both positive and negative ionization modes for data acquisition．Based on the accurate mass information of precursor and fragment ions and online database matching，a total of 64 active ingredients and 32 common active ingredients were identified in the white tea and Pu’er raw tea samples，in which 16 active ingredients were identified only in white tea samples，while another 16 active ingredients solely occurred in Pu’er raw tea samples．They were mainly classified into six categories，including amino acids，organic acids，flavonoids，coumarins，alkaloids and nucleosides．The normalization method was used to determine the relative contents of the 64 active ingredients．This method could be used to analyze the active components in tea，providing a scientific basis for further research on the health mechanism of white tea and Pu’er raw tea，and also providing a technical support for the supervision of tea quality and safety.

关键词

超高效液相色谱高分辨质谱白茶普洱生茶

Keywords

ultra-high performance liquid chromatographyhigh-resolution mass spectrometrywhite teaPu’er raw tea

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