Construction and Application of a Bimetallic Co-regulated G-triplex/Hemin Colorimetric Sensing Probe

ZHANG Shan; ZHANG Yan; GAO Yu-meng; HUANG Yao-yao; CAI Hui-shan; WEI Yuan; SU Xiao-dong; ZHOU Qian-yu

doi:10.19969/j.fxcsxb.22112103

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Construction and Application of a Bimetallic Co-regulated G-triplex/Hemin Colorimetric Sensing Probe

Scientific Papers | 更新时间：2023-06-15

- Construction and Application of a Bimetallic Co-regulated G-triplex/Hemin Colorimetric Sensing Probe
- Journal of Instrumental Analysis Vol. 42, Issue 6, Pages: 707-714(2023)
- 作者机构：
  
  重庆科技学院化学化工学院，重庆 401331
- 作者简介：
- 基金信息：
- DOI：10.19969/j.fxcsxb.22112103
  CLC：
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张珊,张燕,高渝萌等.双金属协同调控G-三链体/血红素比色传感探针的构筑及应用[J].分析测试学报,2023,42(06):707-714.

ZHANG Shan,ZHANG Yan,GAO Yu-meng,et al.Construction and Application of a Bimetallic Co-regulated G-triplex/Hemin Colorimetric Sensing Probe[J].Journal of Instrumental Analysis,2023,42(06):707-714.
张珊,张燕,高渝萌等.双金属协同调控G-三链体/血红素比色传感探针的构筑及应用[J].分析测试学报,2023,42(06):707-714. DOI： 10.19969/j.fxcsxb.22112103.

ZHANG Shan,ZHANG Yan,GAO Yu-meng,et al.Construction and Application of a Bimetallic Co-regulated G-triplex/Hemin Colorimetric Sensing Probe[J].Journal of Instrumental Analysis,2023,42(06):707-714. DOI： 10.19969/j.fxcsxb.22112103.

摘要

该文首次将一条富含G碱基的DNA序列（5′-CTGGGAGGGAGGGA-3′）与血红素结合，形成的G-三链体/血红素复合物具有类过氧化氢酶活性，能够催化H,2,O,2,的氧化反应，使反应底物2，2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）由无色变为绿色。通过对金属离子的筛选，发现K,+,和Sr,2+,同时存在能稳定G-三链体结构，基于此构筑了双金属协同调控的G-三链体/血红素比色传感探针，并将其用于H,2,O,2,的定量分析。在最佳实验条件下，溶液的吸光度与H,2,O,2,浓度在0.5 ~ 4.5 mmol/L范围内呈现良好的线性关系，检出限为0.07 mmol/L。将该方法用于牛奶中H,2,O,2,的检测，加标回收率为92.5% ~ 103%，相对标准偏差为1.2% ~ 5.4%。该探针具有碱基序列短、成本低廉、适用性强等诸多优势，为G-三链体功能化核酸探针的研究提供了新的思路。

Abstract

A bimetallic co-regulated G-triplex/hemin colorimetric sensing probe for the quantitative analysis of H,2,O,2, was constructed in this paper．Firstly，a G-rich DNA sequence（5′-CTGGGAGGGAGGGA-3′） was bound to hemin，forming a G-triplex/hemin complex with peroxidase-like activity that catalyzes the oxidation of H,2,O,2,，causing the reaction substrate to change from colorless to green，which was used as the output module of the sensor．Then by screening the metal ions，it was found that the G-triplex structure could be stabilized in the simultaneous presence of K,+, and Sr,2+,．Under the optimal experimental conditions，the absorbance of the solution showed a good linear relationship with H,2,O,2, in the concentration range of 0.5－4.5 mmol/L，and the limit of detection was 0.07 mmol/L．The method was used for the determination of H,2,O,2, in milk．The spiked recoveries ranged from 92.5% to 103%，with the relative standard deviations of 1.2%－5.4%．With the advantages of short base sequence，low cost and high applicability，the probe provides a new idea for the research of G-triplex functionalized nucleic acid probes.

关键词

G-三链体血红素钾离子锶离子比色探针协同调控

Keywords

G-triplexheminK+Sr2+colorimetric probeco-regulated

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Institute of Resources and Environment，Henan Polytechnic University，Jiaozuo

Hangzhou Institute of Medicine（HIM），Chinese Academy of Sciences，Hangzhou

Institute of Resources and Environment，Henan Polytechnic University

Hangzhou Institute of Medicine（HIM），Chinese Academy of Sciences

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